波谱形变定量理论结合核壳型颗粒用于生物样本中6-硫鸟嘌呤的检测

引用本文: 王颖琦, 陈瑶, 陈增萍. 波谱形变定量理论结合核壳型颗粒用于生物样本中6-硫鸟嘌呤的检测[J]. 分析化学, 2020, 48(2): 269-274. doi: 10.19756/j.issn.0253-3820.191400 shu

Citation: WANG Ying-Qi, CHEN Yao, CHEN Zeng-Ping. Quantification of 6-Thioguanine in Biological Samples by Spectral Shape Deformation Quantitative Theory Combined with Core-shell Nanoparticles[J]. Chinese Journal of Analytical Chemistry, 2020, 48(2): 269-274. doi: 10.19756/j.issn.0253-3820.191400 shu

波谱形变定量理论结合核壳型颗粒用于生物样本中6-硫鸟嘌呤的检测

1.
化学生物传感与计量学国家重点实验室, 湖南大学化学化工学院, 长沙 410082;
2.
湖南省生物医用纳米材料与器件重点实验室, 湖南工业大学生命科学和化学学院, 株洲 412008
基金项目:
本文系国家自然科学基金项目（Nos.21705044，21775038）和湖南省自然科学基金项目（No.2018JJ3114）资助

摘要: 制备了Au-core@4-巯基苯甲酸@Ag-shell核壳型纳米颗粒，结合波谱形变定量分析理论，采用表面增强拉曼光谱（SERS）定量分析了尿液和红细胞样本中6-硫鸟嘌呤（6-TG）。实验结果表明，核壳型纳米颗粒和波谱形变定量分析理论相结合可以有效消除SERS增强基底表面的"热点"数目及其分布等因素的变化对SERS信号的乘子效应影响，从而实现尿液和红细胞样本中6-TG的灵敏、准确定量分析。本方法对6-TG的检出限（3σ）和定量限（10σ）分别为1 nmol/L和3 nmol/L，加标回收率在95.6%~106.7%之间。相较于传统的6-TG分析方法（如高效液相色谱法），本方法具有简单、灵敏、快速等优点，为复杂体系中6-TG的常规定量分析提供了参考。

关键词:

English

Quantification of 6-Thioguanine in Biological Samples by Spectral Shape Deformation Quantitative Theory Combined with Core-shell Nanoparticles

1.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China;
2.
Hunan Key Lab of Biomedical Materials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412008, China
Received Date: 13 July 2019
Revised Date: 03 December 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21705044, 21775038) and the Natural Science Foundation of Hunan Province, China (No. 2018JJ3114).

Abstract: The core-shell nanoparticles (i.e., Au-core@4-mercaptobenzoic acid@Ag-shell) were combined with spectral shape deformation (SSD) quantitative model for quantification of 6-thioguanine (6-TG) in urine and red blood cell lysate samples. Experimental results showed that the combination of core-shell nanoparticles with SSD model could effectively remove the detrimental multiplicative effects on samples' surface enhanced Raman spectroscopy (SERS) signals caused by the variations of the number of "hot spots" and their distribution near/on the surfaces of SERS enhancing substrates, and hence realized the accurate quantitative determination of 6-TG in urine and red blood cell lysate samples with recovery of 95.6%-106.7%. The limit of detection (3σ) and limit of quantification (10σ) for 6-TG were estimated to be about 1 and 3 nmol/L, respectively. Compared with conventional methods for analysis of 6-TG such as high performance liquid chromatography (HPLC), the proposed method had many advantages such as simplicity, rapidity and high sensitivity, and had great potential in routine quantitative analysis of 6-TG in complex samples.

Key words:

Surface enhanced Raman spectroscopy
/ 6-Thioguanine
/ Core-shell nanoparticles
/ Spectral shape deformation quantitative theory

1. [1]
  Harms D O, Janka-Schaub G E. Leukemia,2000,14(12):2234-2239
2. [2]
  Hawwa A F, Millership J S, Collier P S, McElnay J C. J. Pharm. Biomed. Anal., 2009, 49(2):401-409
3. [3]
  Al-Ghobashy M A, Hassan S A, Abdelaziz D H, Elhosseiny N M, Sabry N A, Attia A S, El-Sayed M H. J. Chromatogr. B, 2016, 1038:88-94
4. [4]
  Unal D N, Eksin E, Erdem A. Anal. Lett., 2018, 51(1-2):265-278
5. [5]
  Zeng H, Li L, Ding Y, Zhuang Q. Talanta, 2018, 178:879-885
6. [6]
  Kneipp K, Wang Y, Kneipp H, Perelman L T, Itzkan I, Dasari R, Feld M S. Phys. Rev. Lett., 1997, 78(9):1667-1670
7. [7]
  Lorén A, Engelbrektsson J, Eliasson C, Josefson M, Abrahamsson J, Johansson M, Abrahamsson K. Anal. Chem., 2004, 76(24):7391-7395
8. [8]
  Chen Z P, Xiong S, Zuo Q, Shi C X. J. Chemometr., 2018, 32(11):e2913
9. [9]
  Chen Y, Chen Z P, Zuo Q, Shi C X, Yu R Q. Anal. Chim. Acta, 2015, 887:45-50
10. [10]
  Chen Y, Chen Z P, Jin J W, Yu R Q. Chemometr. Intell. Lab. Syst., 2015, 142:166-171
11. [11]
  Xia T H, Chen Z P, Chen Y, Jin J W, Yu R Q. Anal. Methods, 2014, 6(7):2363-2370
12. [12]
  Chen Y, Chen Z P, Long S Y, Yu R Q. Anal. Chem., 2014, 86(24):12236-12242
13. [13]
  Zhang X Q, Li S X, Chen Z P, Chen Y, Yu R Q. Chemometr. Intell. Lab. Syst., 2018, 177:47-54
14. [14]
  Frens G. Nat. Phys. Sci., 1973, 241(105):20-22
15. [15]
  Feng Y, Wang Y, Wang H, Chen T, Tay Y Y, Yao L, Yan Q, Li S, Chen H. Small, 2012, 8(2):246-251
16. [16]
  Ma W, Fang Y, Hao G, Wang W. Chin. J. Chem. Phys., 2010, 23(6):659-663
17. [17]
  Cordes M M, Walter J L. Spectrochim. Acta A, 1968, 24(9):1421-1435
18. [18]
  Giese B, McNaughton D. Biopolymers, 2003, 72(6):472-489
19. [19]
  Szeghalmi A V, Leopold L, Pinzaru S, Chis V, Silaghi-Dumitrescu I, Schmitt M, Popp J, Kiefer W. Biopolymers, 2005, 78(6):298-310
20. [20]
  Jin J W, Chen Z P, Li L M, Steponavicius R, Thennadil S N, Yang J. Anal. Chem., 2011,84(1):320-326
21. [21]
  Geladi P, Kowalski B R. Anal. Chim. Acta, 1986, 185:1-17
22. [22]
  WU Yu-Heng, HUANG Min, TANG Li-Fen, ZHANG Jian-Ping, CAI Kui-Hua. Chinese J. Pharm. Anal., 2002, 3:237-239 吴珏珩, 黄民, 汤丽芬, 张建萍, 蔡葵花.药物分析杂志, 2002, 3:237-239

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 11
HTML全文浏览量: 0

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

波谱形变定量理论结合核壳型颗粒用于生物样本中6-硫鸟嘌呤的检测

English

Quantification of 6-Thioguanine in Biological Samples by Spectral Shape Deformation Quantitative Theory Combined with Core-shell Nanoparticles

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处