Citation: HAN Ya-Chen, FENG Liu-Xing, LI Hong-Mei, XIONG Jin-Ping. Quantification of Human Serum Transferrin Based on High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry with Gold Nanoparticles Labeling[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(2): 206-214. doi: 10.19756/j.issn.0253-3820.191394 shu

Quantification of Human Serum Transferrin Based on High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry with Gold Nanoparticles Labeling

1.
College of Material Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;
2.
National Institute of Metrology, Beijing 100029, China

Received Date: 8 July 2019
Revised Date: 24 October 2019

Fund Project: This work was supported by the National Key Research and Development Program (No. 2017YFF0205402) and the National Natural Science Foundation of China (No.11475163).

A simple and sensitive method for quantification of transferrin (Tf) in human serum by high performance liquid chromatography (HPLC) coupled with inductively coupled plasma mass spectrometer (ICP-MS) with gold nanoparticles (AuNPs) labeling was established. Accurate quantification of standard Tf and Tf in human serum were achieved by measuring the concentration of AuNPs labeled on Tf. Tf could be incubated with most amount of AuNPs to form AuNPs-Tf in phosphate buffer (pH 6.8). Since gold atoms labeled to the Tf molecule could enhance the signals, accurate quantification of Tf in human serum could be achieved by gold signal measurement with ICP-MS after AuNPs-Tf was separated by HPLC. Good linear correlation coefficient (R²=0.9959) was obtained between Au peak area on AuNPs-Tf and concentration of Tf with a wide linear response over 3 orders of magnitude, and the limit of detection was 6 ng/mL. The developed method was successfully applied for detection of Tf in human serum certified reference material (ERM-DA470/IFCC) after being verified by standard Tf. Good agreement was achieved and the quantitative recovery was in the range of 95.2%-102.6%. Due to signal amplification of AuNPs and ultra-sensitive detection of ICP-MS, the sensitivity and accuracy were improved significantly compared to traditional enzyme-linked immunosorbent assay. Moreover, the measurement results could be directly traced to SI unit.
- High performance liquid chromatography-inductively coupled plasma-mass spectrometry,
- Gold nanoparticles,
- Transferrin
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1.
沈阳化工大学材料科学与工程学院沈阳 110142