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Citation: HUO Zhong-Zhong, FENG Liu-Xing, LI Hong-Mei, XIONG Jin-Ping. Absolute Quantification of Beta Amyloid Peptide Based on Sulfur Isotope Dilution Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(12): 1931-1937. doi: 10.19756/j.issn.0253-3820.191384 shu

Absolute Quantification of Beta Amyloid Peptide Based on Sulfur Isotope Dilution Mass Spectrometry

1.
College of Material Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;
2.
National Institute of Metrology, Beijing 100029, China

Received Date: 8 July 2019
Revised Date: 20 October 2019

Fund Project: This work was supported by the National Key Research and Development Program of China (No. 2017YFF0205402) and the National Natural Science Foundation of China(No. 11475163)

The accurate quantification of β-amyloid peptides was achieved by the accurate measurement of sulfur elements in β-amyloid peptides based on high performance liquid chromatography isotope dilution inductively coupled plasmon mass spectrometry (HPLC-ID-ICPMS). Aβ₄₂ in β-amyloid peptide was separated from impurities by optimizing liquid chromatography conditions, and then the eluent was mixed online with enriched ³⁴S diluent. In view of the influence of enriched ³⁴S diluent on ³²S/³⁴S signal strength and sensitivity, the flow rate of ³⁴S diluent was optimized. According to the formula of isotope dilution method and the number of S elements in Aβ₄₂, the content of Aβ₄₂ in β amyloid peptide was (0.763±0.0044) g/g. The linear correlation coefficient was 0.999 within the range of 5-60 μg/g, the detection limit was 140 pg/g, and the quantitative limit was 467 pg/g. The recovery of the Aβ₄₂ was between 98% and 105%, and the relative standard deviation was less than 6%. The measurement result of this sample by amino acid hydrolysis method was (0.768±0.0091) g/g, which was in good consistency with the result by this method.
- β-Amyloid peptide,
- Sulfur element,
- Quantitative determination,
- Isotope dilution
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