中性解吸-电喷雾萃取电离质谱直接分析水涝胁迫下烟草的代谢产物

引用本文: 吴栋, 于腾辉, 李享, 崔萌, 郝影宾, 刘丽华, 罗丽萍. 中性解吸-电喷雾萃取电离质谱直接分析水涝胁迫下烟草的代谢产物[J]. 分析化学, 2020, 48(1): 121-128. doi: 10.19756/j.issn.0253-3820.191376 shu

Citation: WU Dong, YU Teng-Hui, LI Xiang, CUI Meng, HAO Ying-Bin, LIU Li-Hua, LUO Li-Ping. Direct Analysis of Nicotiana Tabacum Metabolites under Waterlogging Stress by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 121-128. doi: 10.19756/j.issn.0253-3820.191376 shu

中性解吸-电喷雾萃取电离质谱直接分析水涝胁迫下烟草的代谢产物

吴栋 ^1, ,
于腾辉 ^1, ,
李享 ^1, ,
崔萌 ^1, ,
郝影宾 ^1, ,
刘丽华 ^1, ,
罗丽萍 ^1,2,

1.
南昌大学生命科学学院, 南昌 330031;
2.
南昌大学, 食品科学与技术国家重点实验室, 南昌 330047
基金项目:
本文系江西省重点研发计划项目（No.20171ACF60001）、江西省科技创新人才项目（No.20165BCB18005）和国家自然科学基金项目（No.31772067）资助

摘要: 烟草（Nicotiana tabacum L.）是植物科学研究的模式植物和我国重要的经济作物，水涝胁迫是影响烟草品质和产量的重要胁迫因子之一。本研究采用中性解吸-电喷雾萃取电离质谱（ND-EESI-MS）技术，在无需复杂的预处理的条件下，利用烟草中常见的丝氨酸作为优化离子，对萃取剂种类及流速、电喷雾电压、样品端辅助气压、雾化气压和离子传输管温度等仪器参数进行优化。在最优的条件下，共检出水涝胁迫下烟草中丝氨酸、脯氨酸、谷氨酸、丙氨酰甘氨酸、亮氨酸、天冬酰胺、组氨酸、儿茶酚、麦芽酚、肉桂酸、咖啡酸、阿魏酸、白藜芦醇、山奈酚、槲皮素、绿原酸、烟碱、降烟碱、苯乙醛、柠檬醛、苹果酸和β-大马烯酮共22种代谢产物。在应对水涝胁迫时，烟草中脯氨酸、谷氨酸、丝氨酸和组氨酸等初生代谢产物含量增加，绿原酸、阿魏酸、咖啡酸、儿茶酚、苯乙醛、烟碱和降烟碱等次生代谢产物含量也呈上升趋势。本研究从代谢组学层面上揭示水涝胁迫下烟草代谢产物的变化，为进一步了解烟草响应水涝胁迫提供了理论基础。ND-EESI-MS应用于水涝胁迫下烟草中代谢物检测，具有灵敏度高、检测范围广、可实现高通量快速检测等优点，单个样品的测定时间不超过1 min，为烟草响应水涝胁迫的代谢机理研究提供了新的植物代谢组学技术。

关键词:

English

Direct Analysis of Nicotiana Tabacum Metabolites under Waterlogging Stress by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry

1.
School of Life Sciences, Nanchang University, Nanchang 330031, China;
2.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
Received Date: 04 July 2019
Revised Date: 11 November 2019
Fund Project:
This work was supported by the Jiangxi Province Key Research and Development Plan (No.20171ACF60001), the Jiangxi Province Science and Technology Innovation Talent Project (No.20165BCB18005) and the National Natural Science Foundation of China (No.31772067).

Abstract: Tobacco (Nicotiana tabacum L.) is a model plant for plant science research and one of the important economic crops, and waterlogging stress is one of the most important factors affecting the quality and yield of Nicotiana tabacum. In this study, the instrument parameters such as the type of extractant, the flow rate of extractant reagent, the electrospray voltage, the pressure of sample auxiliary, the pressure of extractant reagent and the temperature of ion transfer tube were optimized by serine, which was common in Nicotiana tabacum as an optimized ion. A total of 22 metabolites of Nicotiana tabacum were obtained under waterlogging stress by neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) without any sample pretreatment, including serine, proline, glutamic, D,L-alanyl-glycine, leucine, asparagine, histidine, catechol, maltol, cinnamic acid, caffeic acid, ferulic acid, resveratrol, kaempferol, quercetin chlorogenic acid, nicotine, nornicotine, phenylacetaldehyde, citral, malic acid adn β-damascenone. In response to waterlogged stress, the contents of proline, glutamic acid, serine, histidine about primary metabolites in Nicotiana tabacum were increased, and the contents of secondary metabolites such as chlorogenic acid, ferulic acid, caffeic acid, catechol, phenylacetaldehyde, nicotine and nornicotine were also increased. The results exhibited the metabolites changes of Nicotiana tabacum under waterlogging stress, and thus provided a theoretical basis for further understanding the response to waterlogging stress in Nicotiana tabacum. Accordingly, the application of ND-EESI-MS in plant in-situ detection had many advantages such as high sensitivity, wide detection range, high throughput and rapid detection (less than 1 minute for detection of a single sample). Also it provided a new plant metabolomics technique for enriching the metabolic mechanism of Nicotiana tabacum in response to waterlogging stress.

Key words:

Neutral desorption-extractive electrospray ionization mass spectrometry
/ Nicotiana tabacum L
/ Waterlogging stress
/ Metabolomics

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中性解吸-电喷雾萃取电离质谱直接分析水涝胁迫下烟草的代谢产物

English

Direct Analysis of Nicotiana Tabacum Metabolites under Waterlogging Stress by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry

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中国化学会秘书处

中性解吸-电喷雾萃取电离质谱直接分析水涝胁迫下烟草的代谢产物

English

Direct Analysis of Nicotiana Tabacum Metabolites under Waterlogging Stress by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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