Citation: WU Dong, YU Teng-Hui, LI Xiang, CUI Meng, HAO Ying-Bin, LIU Li-Hua, LUO Li-Ping. Direct Analysis of Nicotiana Tabacum Metabolites under Waterlogging Stress by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(1): 121-128. doi: 10.19756/j.issn.0253-3820.191376 shu

Direct Analysis of Nicotiana Tabacum Metabolites under Waterlogging Stress by Neutral Desorption-Extractive Electrospray Ionization Mass Spectrometry

WU Dong ^1, ,
YU Teng-Hui ¹ ,
LI Xiang ¹ ,
CUI Meng ¹ ,
HAO Ying-Bin ¹ ,
LIU Li-Hua ¹ ,
LUO Li-Ping ^1,2

1.
School of Life Sciences, Nanchang University, Nanchang 330031, China;
2.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received Date: 4 July 2019
Revised Date: 11 November 2019

Fund Project: This work was supported by the Jiangxi Province Key Research and Development Plan (No.20171ACF60001), the Jiangxi Province Science and Technology Innovation Talent Project (No.20165BCB18005) and the National Natural Science Foundation of China (No.31772067).

Tobacco (Nicotiana tabacum L.) is a model plant for plant science research and one of the important economic crops, and waterlogging stress is one of the most important factors affecting the quality and yield of Nicotiana tabacum. In this study, the instrument parameters such as the type of extractant, the flow rate of extractant reagent, the electrospray voltage, the pressure of sample auxiliary, the pressure of extractant reagent and the temperature of ion transfer tube were optimized by serine, which was common in Nicotiana tabacum as an optimized ion. A total of 22 metabolites of Nicotiana tabacum were obtained under waterlogging stress by neutral desorption-extractive electrospray ionization mass spectrometry (ND-EESI-MS) without any sample pretreatment, including serine, proline, glutamic, D,L-alanyl-glycine, leucine, asparagine, histidine, catechol, maltol, cinnamic acid, caffeic acid, ferulic acid, resveratrol, kaempferol, quercetin chlorogenic acid, nicotine, nornicotine, phenylacetaldehyde, citral, malic acid adn β-damascenone. In response to waterlogged stress, the contents of proline, glutamic acid, serine, histidine about primary metabolites in Nicotiana tabacum were increased, and the contents of secondary metabolites such as chlorogenic acid, ferulic acid, caffeic acid, catechol, phenylacetaldehyde, nicotine and nornicotine were also increased. The results exhibited the metabolites changes of Nicotiana tabacum under waterlogging stress, and thus provided a theoretical basis for further understanding the response to waterlogging stress in Nicotiana tabacum. Accordingly, the application of ND-EESI-MS in plant in-situ detection had many advantages such as high sensitivity, wide detection range, high throughput and rapid detection (less than 1 minute for detection of a single sample). Also it provided a new plant metabolomics technique for enriching the metabolic mechanism of Nicotiana tabacum in response to waterlogging stress.
- Neutral desorption-extractive electrospray ionization mass spectrometry,
- Nicotiana tabacum L,
- Waterlogging stress,
- Metabolomics
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