孔雀石绿分子印迹电化学传感器的制备与应用

引用本文: 韦寿莲, 吴嘉喻, 黄象金, 谢春生. 孔雀石绿分子印迹电化学传感器的制备与应用[J]. 分析化学, 2020, 48(1): 145-152. doi: 10.19756/j.issn.0253-3820.191349 shu

Citation: WEI Shou-Lian, WU Jia-Yu, HUANG Xiang-Jin, XIE Chun-Sheng. Preparation and Application of Malachite Green Electrochemical Sensor Based on Molecularly Imprinted Polymer[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 145-152. doi: 10.19756/j.issn.0253-3820.191349 shu

孔雀石绿分子印迹电化学传感器的制备与应用

1.
肇庆学院环境与化学工程学院, 肇庆 526061;
2.
肇庆市食品检验所, 肇庆 526060
基金项目:
本文系国家大学生创新训练项目（No.201810580019）和肇庆市科技计划项目（No.2018N017）资助

摘要: 以孔雀石绿（MG）为模板分子，通过电聚合邻苯二胺，在多孔碳纳米纤维（PCNF）修饰的玻碳电极表面制备了可特异性识别MG的分子印迹传感器。采用差分脉冲伏安法（DPV）、循环伏安法（CV）和电化学阻抗法（EIS）表征了传感器的电化学性能，优化了PCNF的滴涂量、模板分子与功能单体的摩尔比、CV扫描圈数、洗脱剂、洗脱时间、样品溶液pH值及孵化时间等实验参数。在最佳条件下，DPV峰电流强度与MG浓度在0.10~10.0 nmol/L范围内呈良好的线性关系，检出限为0.042 nmol/L（3S/k）。此传感器具有良好的选择性、重现性和稳定性。将本方法应用于市售新鲜草鱼及养殖水样品中MG的含量测定，加标回收率为82.0%~96.5%。

关键词:

English

Preparation and Application of Malachite Green Electrochemical Sensor Based on Molecularly Imprinted Polymer

1.
College of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, China;
2.
Zhaoqing Institute for Food Control, Zhaoqing 526060, China
Received Date: 23 June 2019
Revised Date: 19 November 2019
Fund Project:
This work was supported by the College Students' Innovative Entrepreneurial Training Program from Zhaoqing University (No. 201810580019) and the Science and Technology Planning Project of Zhaoqing City (No. 2018N017).

Abstract: A molecularly imprinted electrochemical sensor for specific recognition of malachite green (MG) was prepared by electropolymerization of o-phenylenediamine on the surface of porous carbon nanofiber (PCNF) modified glassy carbon electrode with MG as template molecule. The electrochemical performance of the sensor was characterized by differential pulse voltammetry (DPV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The experimental parameters, such as the amount of PCNF, molar ratio of template molecule to functional monomer, scan cycle of CV, eluent, elution time, pH value of sample solution and incubation time, were optimized. Under optimal conditions, the peak current was linear to MG concentration in the range of 0.10-10.0 nmol/L, with a detection limit of 0.042 nmol/L. The prepared sensor possessed excellent selectivity, reproducibility and stability, and was applied to the detection of MG in commercially available fresh grass carp and aquaculture water samples with recoveries of 82.0%-96.5%.

Key words:

Molecularly imprinted electrochemical sensor
/ Electropolymerization
/ Porous carbon nanofiber
/ Malachite green

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

孔雀石绿分子印迹电化学传感器的制备与应用

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Preparation and Application of Malachite Green Electrochemical Sensor Based on Molecularly Imprinted Polymer

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