Citation: ZOU Xiao-Bo, JIANG Cai-Ping, LI Zhi-Hua, SUN Yue, SHI Ji-Yong, LI Yan-Xiao, HUANG Xiao-Wei, ZHANG Di, HU Xue-Tao, ZHAI Xiao-Dong, WEI Xiao-Ou. A “Turn on-off” Fluorescent Probe Based on Aptamer/Gold Nanoparticles for Rapid Detection of Isocarbophos[J]. Chinese Journal of Analytical Chemistry, ;2020, 48(4): 507-515. doi: 10.19756/j.issn.0253-3820.191335 shu

A “Turn on-off” Fluorescent Probe Based on Aptamer/Gold Nanoparticles for Rapid Detection of Isocarbophos

1.
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
2.
Analytical Instrumentation Center, Jiangsu University, Zhenjiang 212013, China

Received Date: 14 June 2019
Revised Date: 21 February 2020

Fund Project: This work was supported by the National Key Research and Development Program of China (Nos. 2017YFC1600806, 2016YFD0401104), the National Natural Science Foundation of China (Nos. 31801631, 31671844, 31601543), the Natural Science Foundation of Jiangsu Province (Nos. BK20160506, BE2016306, BK20180865) and the International Science and Technology Cooperation Project of Jiangsu Province (No. BZ2016013).

A "turn on-off" fluorescent probe based on aptamer/gold nanoparticles(AuNPs) was developed for rapid, highly sensitive and selective detection of isocarbophos (ICP). Hairpin complementary strand (cDNA) with a fluorescein label (FAM) at 5' end was attached to the surface of AuNPs through thiol group. Subsequently, ICP-binding aptamer (ICP-Aptamer) was added and hybridized with cDNA, leading to open of hairpin assembly and "turn on" of fluorescent signal quenched by AuNPs. ICP-Aptamer specifically bound to the target ICP with restoration of configuration and "turn off" of the fluorescent signal. The properties of nanoparticles were characterized by transmission electron microscopy, ultraviolet-visible absorption spectroscopy and zeta potential. Experimental parameters influencing fluorescence inhibition rate, including pH value, concentration of ICP-Aptamer, incubation time and incubation temperature, were investigated and optimized. The corresponding fluorescence inhibition rate had a good linear relationship with concentration of ICP in the range of 0.02-10 μmol/L, and the limit of detection was 17.8 nmol/L. Furthermore,the fluorescent probe was applied to determination of ICP in rice and spinach samples. The recoveries were from 92.9% to 107.0% and the relative standard deviations were lower than 4.2%. It could be concluded that the fluorescence probe had good application potential for detection of pesticides and supervision of food safety.
- Fluorescent probe,
- Turn on-off,
- Aptamer,
- Gold nanoparticles,
- Isocarbophos
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