基于不同链长DNA-金纳米粒子的阵列比色传感器对多种重金属离子的鉴别

引用本文: 边孟孟, 徐梦, 袁亚利, 聂瑾芳. 基于不同链长DNA-金纳米粒子的阵列比色传感器对多种重金属离子的鉴别[J]. 分析化学, 2020, 48(7): 863-870. doi: 10.19756/j.issn.0253-3820.191325 shu

Citation: BIAN Meng-Meng, XU Meng, YUAN Ya-Li, NIE Jin-Fang. Colorimetric Array Sensor for Discrimination of Multiple Heavy Metal Ions Based on Different Lengths of DNA-AuNPs[J]. Chinese Journal of Analytical Chemistry, 2020, 48(7): 863-870. doi: 10.19756/j.issn.0253-3820.191325 shu

基于不同链长DNA-金纳米粒子的阵列比色传感器对多种重金属离子的鉴别

桂林理工大学化学与生物工程学院, 桂林 541004
基金项目:
本文系国家自然科学基金项目（No.21765005）、广西壮族自治区自然科学基金项目（No.2018GXNSFAA138047）、广西壮族自治区科学技术基金项目（No.guikeAD19110096）和广西壮族自治区教育厅项目（No.2018KY0260）资助

摘要: 构建了一种基于DNA-金纳米粒子（DNA-AuNPs）的无标记比色阵列传感器，用于多种金属离子的鉴别。以不同长度单链DNA（ssDNA）链（15A、30A和45AT）作为非特异性受体，分别与AuNPs结合，构成3个复合体系（15A DNA-AuNPs、30A DNA-AuNPs、45AT DNA-AuNPs），作为此传感器的3个阵列传感元件。在高浓度盐的存在下，不同的金属离子可触发DNA保护的AuNPs显示出不同的聚集行为并呈现各种颜色变化。以紫外-可见吸收光谱作为传感器的响应信号，通过主成分分析（PCA）和线性判别分析（LDA）对此比色传感器的响应信号进行分析。实验结果表明，11种不同重金属离子（Ag⁺、Pb²⁺、Zn²⁺、Cu²⁺、Cd²⁺、Mn²⁺、Ni²⁺、Co²⁺、Hg²⁺、Bi³⁺、Cr³⁺）在0.5 μmol/L水平下，通过LDA可得到很好的区分。

关键词:

English

Colorimetric Array Sensor for Discrimination of Multiple Heavy Metal Ions Based on Different Lengths of DNA-AuNPs

College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China
Received Date: 10 June 2019
Revised Date: 09 May 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21765005), the Guangxi Natural Science Foundation (No. 2018GXNSFAA138047), the Science and Technology Project of Guangxi (No. guikeAD19110096), and the Foundation of Guangxi Education Office (No. 2018KY0260).

Abstract: A label-free colorimetric sensor based on DNA-gold nanoparticles (DNA-AuNPs) was constructed for discrimination of multiple heavy metal ions. Different lengths of ssDNA strands (15A, 30A and 45AT) were used as non-specific receptors, which were combined with AuNPs to form 15A DNA-AuNPs, 30A DNA-AuNPs and 45AT DNA-AuNPs complex and acted as three sensing elements of this colorimetric sensor. It was found that different metal ions could trigger the DNA-protected AuNPs and showed different aggregation behaviors along with various color changes in the presence of high concentrations salt. The different absorption of AuNPs was used as the response signal of the sensor, which was then analyzed by principal component analysis (PCA) and linear discriminant analysis (LDA). The experimental results showed that 11 kinds of heavy metal ions (Ag⁺, Pb²⁺, Zn²⁺, Cu²⁺, Cd²⁺, Mn²⁺, Ni²⁺, Co²⁺, Hg²⁺, Bi³⁺ and Cr³⁺) could be well distinguished by LDA at the level of 0.5 μmol/L.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于不同链长DNA-金纳米粒子的阵列比色传感器对多种重金属离子的鉴别

English

Colorimetric Array Sensor for Discrimination of Multiple Heavy Metal Ions Based on Different Lengths of DNA-AuNPs

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中国化学会秘书处

基于不同链长DNA-金纳米粒子的阵列比色传感器对多种重金属离子的鉴别

English

Colorimetric Array Sensor for Discrimination of Multiple Heavy Metal Ions Based on Different Lengths of DNA-AuNPs

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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