串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

陈蜜 岳仁叶 李智 王刚林 马楠

引用本文: 陈蜜,  岳仁叶,  李智,  王刚林,  马楠. 串联的纳米传感器用于癌细胞中miRNA的超灵敏检测[J]. 分析化学, 2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu
Citation:  CHEN Mi,  YUE Ren-Ye,  LI Zhi,  WANG Gang-Lin,  MA Nan. Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu

串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

  • 基金项目:

    本文系国家自然科学基金项目(Nos.21475093,21522506)资助

摘要: MicroRNA(miRNA)的检测在癌症诊断和治疗中具有重要意义。本研究设计了DNA序列,组装了3组金纳米粒子-量子点复合物,基于此叠加构建了以熵驱动催化循环为模型的单重、双重和多重循环放大的3种纳米传感器。叠加的传感器中,上层复合物催化循环解组装输出的产物作为下层复合物的催化链,引发其解组装,释放更多荧光信号,实现了多重放大。传感器中每叠加一个核酸催化循环体系,检测限降低一个数量级,最终构建的传感器的检测限达到fmol/L级。本研究通过串联熵驱动的核酸催化循环体系,构建了动态可调的、可定量检测细胞中不同表达水平miRNA的新型纳米传感器。

English


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  • 收稿日期:  2019-06-04
  • 修回日期:  2019-11-14
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