串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

引用本文: 陈蜜, 岳仁叶, 李智, 王刚林, 马楠. 串联的纳米传感器用于癌细胞中miRNA的超灵敏检测[J]. 分析化学, 2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu

Citation: CHEN Mi, YUE Ren-Ye, LI Zhi, WANG Gang-Lin, MA Nan. Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu

串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

苏州大学材料与化学化工学部, 苏州 215123
基金项目:
本文系国家自然科学基金项目（Nos.21475093，21522506）资助

摘要: MicroRNA（miRNA）的检测在癌症诊断和治疗中具有重要意义。本研究设计了DNA序列，组装了3组金纳米粒子-量子点复合物，基于此叠加构建了以熵驱动催化循环为模型的单重、双重和多重循环放大的3种纳米传感器。叠加的传感器中，上层复合物催化循环解组装输出的产物作为下层复合物的催化链，引发其解组装，释放更多荧光信号，实现了多重放大。传感器中每叠加一个核酸催化循环体系，检测限降低一个数量级，最终构建的传感器的检测限达到fmol/L级。本研究通过串联熵驱动的核酸催化循环体系，构建了动态可调的、可定量检测细胞中不同表达水平miRNA的新型纳米传感器。

关键词:

English

Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
Received Date: 04 June 2019
Revised Date: 14 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21475093, 21522506).

Abstract: MicroRNA (miRNA) detection is of considerable significance in the diagnosis and treatment of cancer. The expression level of cancer-related miRNA differs significantly in various cancer cell types, and it remains challenging to detect miRNA at both high and low levels accurately using a conventional sensor with fixed limit of detection and narrow linear detecting range. Herein, three kinds of gold nanoparticle-quantum dots composite were constructed by reasonable DNA sequence design. By stacking these nanocomposites sequentially, three kinds of sensors of single, double and multiple amplifications based on entropy-driven catalytic circuits were successfully established. The product of the upper nanocomposite was served as the catalyst of the downstream nanocomposite to activate disassembly, inducing high-order amplification. With stacking a nucleic acid circuit of the sensor, the detection limit of the sensor was reduced by one order of magnitude. Finally, the nanosensor had a detection limit of fmol/L. By stacking entropy-driving nucleic acid circuits, a novel, dynamically tunable nanosensor was established for quantitatively detecting miRNA with different expression levels in cancer cells.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

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Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells

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