串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

引用本文: 陈蜜, 岳仁叶, 李智, 王刚林, 马楠. 串联的纳米传感器用于癌细胞中miRNA的超灵敏检测[J]. 分析化学, 2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu

Citation: CHEN Mi, YUE Ren-Ye, LI Zhi, WANG Gang-Lin, MA Nan. Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells[J]. Chinese Journal of Analytical Chemistry, 2020, 48(1): 40-48. doi: 10.19756/j.issn.0253-3820.191318 shu

串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

苏州大学材料与化学化工学部, 苏州 215123
基金项目:
本文系国家自然科学基金项目（Nos.21475093，21522506）资助

摘要: MicroRNA（miRNA）的检测在癌症诊断和治疗中具有重要意义。本研究设计了DNA序列，组装了3组金纳米粒子-量子点复合物，基于此叠加构建了以熵驱动催化循环为模型的单重、双重和多重循环放大的3种纳米传感器。叠加的传感器中，上层复合物催化循环解组装输出的产物作为下层复合物的催化链，引发其解组装，释放更多荧光信号，实现了多重放大。传感器中每叠加一个核酸催化循环体系，检测限降低一个数量级，最终构建的传感器的检测限达到fmol/L级。本研究通过串联熵驱动的核酸催化循环体系，构建了动态可调的、可定量检测细胞中不同表达水平miRNA的新型纳米传感器。

关键词:

English

Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
Received Date: 04 June 2019
Revised Date: 14 November 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21475093, 21522506).

Abstract: MicroRNA (miRNA) detection is of considerable significance in the diagnosis and treatment of cancer. The expression level of cancer-related miRNA differs significantly in various cancer cell types, and it remains challenging to detect miRNA at both high and low levels accurately using a conventional sensor with fixed limit of detection and narrow linear detecting range. Herein, three kinds of gold nanoparticle-quantum dots composite were constructed by reasonable DNA sequence design. By stacking these nanocomposites sequentially, three kinds of sensors of single, double and multiple amplifications based on entropy-driven catalytic circuits were successfully established. The product of the upper nanocomposite was served as the catalyst of the downstream nanocomposite to activate disassembly, inducing high-order amplification. With stacking a nucleic acid circuit of the sensor, the detection limit of the sensor was reduced by one order of magnitude. Finally, the nanosensor had a detection limit of fmol/L. By stacking entropy-driving nucleic acid circuits, a novel, dynamically tunable nanosensor was established for quantitatively detecting miRNA with different expression levels in cancer cells.

Key words:

1. [1]
  Wienholds E, Plasterk R H. FEBS Lett.,2005,579(26):5911-5922
2. [2]
  He L, Hannon G J. Nat. Rev. Genet.,2004,5(7):522-531
3. [3]
  Ambros V. Nature,2004,431(7006):350-355
4. [4]
  Dong H, Lei J, Ding L, Wen Y, Ju H, Zhang X. Chem. Rev.,2013,113(8):6207-6233
5. [5]
  Bartel D P. Cell,2009,136(2):215-233
6. [6]
  Bartel D P. Cell,2004,116(2):281-297
7. [7]
  Di Stefano V, Zaccagnini G, Capogrossi M C, Martelli F. Vasc. Pharmacol.,2011,55(4):111-118
8. [8]
  Esquela-Kerscher A, Slack F J. Nat. Rev. Cancer,2006,6(4):259-269
9. [9]
  Calin G A, Croce C M. Nat. Rev. Cancer,2006,6(11):857-866
10. [10]
  Liang D, Meyer L, Chang D W, Lin J, Pu X, Ye Y Q, Gu J, Wu X F, Lu K. Cancer Res.,2010,70(23):9765-9776
11. [11]
  Witwer K W. Clin. Chem.,2015,61(1):56-63
12. [12]
  Kayano M, Higaki S, Satoh J I, Matsumoto K, Matsubara E, Takikawa O, Niida S. Biomarker Res.,2016,4(1):22
13. [13]
  Murakami Y, Yasuda T, Saigo K, Urashima T, Toyoda H, Okanoue T, Shimotohno K. Oncogene,2006,25(17):2537-2545
14. [14]
  Sato Y, Ichihashi T, Nishizawa S, Teramae N. Angew. Chem. Int. Ed.,2012,51(26):6369-6372
15. [15]
  Cheung T H, Quach N L, Charville G W, Liu L, Park L, Edalati A,Yoo B, Hoang P, Rando T A. Nature, 2012,482(7386):524-528
16. [16]
  Hansen T B, Jensen T I, Clausen B H, Bramsen J B, Finsen B, Damgaard C K, Kjems J. Nature,2013,495(7441):384-388
17. [17]
  Benes V, Castoldi M. Methods,2010,50(4):244-249
18. [18]
  Marabita F, de Candia P, Torri A, Tegner J, Abrignani S, Rossi R L. Brief. Bioinform.,2015,17(2):204-212
19. [19]
  Wang F, Lu C H, Willner I. Chem. Rev.,2014,114(5):2881-2941
20. [20]
  Chen J, Tang L, Chu X, Jiang J. Analyst,2017,142(17):3048-3061
21. [21]
  Yin B C, Liu Y Q, Ye B C. J. Am. Chem. Soc.,2012,134(11):5064-5067
22. [22]
  Wu C, Cansiz S, Zhang L, Teng I T, Qiu L, Li J, Tan W. J. Am. Chem. Soc.,2015,137(15):4900-4903
23. [23]
  Khlebtsov N, Dykman L. Chem. Soc. Rev.,2011,40(3):1647-1671
24. [24]
  Seferos D S, Giljohann D A, Hill H D, Prigodich A E, Mirkin C A. J. Am. Chem. Soc.,2007,129(50):15477-15479
25. [25]
  Dulkeith E, Ringler M, Klar T A, Feldmann J, Javier A M, Parak W J. Nano Lett.,2005,5(4):585-589
26. [26]
  Dubertret B, Skourides P, Norris D J, Noireaux V, Brivanlou A H, Libchaber A. Science,2002,298(5599):1759-1762
27. [27]
  Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T. Nat. Methods,2008,5(9):763-775
28. [28]
  ZHANG Tao, LI Zhi, SUN Quan-Hong, MA Nan. Chinese J. Anal. Chem.,2016,44(12):1840-1845 张涛, 李智, 孙权洪, 马楠.分析化学,2016,44(12):1840-1845
29. [29]
  Jou A F J, Lu C H, Ou Y C, Wang S S, Hsu S L, Willner I, Ho J A A. Chem. Sci.,2015,6(1):659-665
30. [30]
  Ma N, Sargent E H, Kelley S O. Nat. Nanotechnol.,2009,4(2):121-125
31. [31]
  He X, Zeng T, Li Z, Wang G, Ma N. Angew. Chem. Int. Ed.,2016,55(9):3073-3076
32. [32]
  Zhang D Y, Turberfield A J, Yurke B, Winfree E. Science,2007,318(5853):1121-1125
33. [33]
  Liang C P, Ma P Q, Liu H, Guo X, Yin B C, Ye B C. Angew. Chem. Int. Ed.,2017,56(31):9077-9081
34. [34]
  Li D, Zhou W, Yuan R, Xiang Y. Anal. Chem.,2017,89(18):9934-9940
35. [35]
  Quan K,Huang J, Yang X H, Yang Y J, Ying L, Wang H, Xie N L, Ou M, Wang K M. Anal. Chem.,2016,88(11):5857-5864
36. [36]
  Yu W W, Qu L, Guo W Z, Peng X G. Chem. Mater.,2003,15(14):2854-2860
37. [37]
  Mitchell P S, Parkin R K, Kroh E M, Fritz B R, Wyman S K, Pogosova-Agadjanyan E L, Peterson A, Noteboom J, O'Briant K C, Allen A, Lin D W, Urban N. Proc. Natl. Acad. Sci. USA,2008,105(30):10513-10518
38. [38]
  Yang Y J, Huang J, Yang X, He X X, Quan K, Xie N L, Qu M, Wang K M. Anal. Chem.,2017,89(11):5850-5856
39. [39]
  Liu Y Q, Zhang M, Yin B C, Ye B C. Anal. Chem.,2012,84(12):5165-5169

扫一扫看文章

计量

PDF下载量: 3
文章访问数: 32
HTML全文浏览量: 1

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

串联的纳米传感器用于癌细胞中miRNA的超灵敏检测

English

Cascaded Nanosensors for Ultrasensitive Detection of miRNA in Cancer Cells

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处