Citation: WANG Zhi-Li, DING Pi, GAO Tian, CHEN Chang-Chong, CAO Yi, SUN Na, PEI Ren-Jun. Capture of Circulating Tumor Cells by Hydrogel-Nanofiber Substrate[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(8): 1162-1169. doi: 10.19756/j.issn.0253-3820.191304 shu

Capture of Circulating Tumor Cells by Hydrogel-Nanofiber Substrate

WANG Zhi-Li ^1,2, ,
DING Pi ^1,2 ,
GAO Tian ^1,2 ,
CHEN Chang-Chong ² ,
CAO Yi ² ,
SUN Na ² ,
PEI Ren-Jun ²

1.
School of Nano Technology and Nano Bionics, University of Science and Technology of China, Hefei 230026, China;
2.
Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

Received Date: 24 May 2019
Revised Date: 10 June 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21575154) and the Science Foundation of Jiangsu Province, China (Nos. BE2016680, BK20180250).

The method for detection of circulating tumor cells (CTCs) is considered as a promising cancer liquid biopsy technique. However, due to the rarity and heterogeneity, it is still a challenging task to capture CTCs with high purity and viability from blood for the following enumeration and molecular analysis. Herein, we fabricated a hydrogel-chitosan nanofiber substrate to effectively capture the target cancer cells, assisted by the poly(carboxybetaine methacrylate) (PCBMA) hydrogel and the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody. The PCBMA hydrogel layer was fabricated by free radical polymerization to provide an antifouling surface, which could help to reduce the non-specific capture of blood cells to improve the capture purity of CTCs. Then, chitosan nanofibers were introduced onto the hydrogel layer to provide a nanostructured surface to improve the capture efficiency. Finally, the capture yield of artificial blood samples on the anti-EpCAM modified hydrogel-nanofiber substrate was up to 79.9%, revealing the potential application of the hydrogel-nanofiber substrate on efficient and sensitive capture of rare circulating tumor cells.
- Circulating tumor cells,
- Efficient capture,
- Nanostructures,
- Hydrogel antifouling surface
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