基于近红外光谱的红提维生素C含量、糖度及总酸含量无损检测方法

引用本文: 高升, 王巧华, 李庆旭, 施行. 基于近红外光谱的红提维生素C含量、糖度及总酸含量无损检测方法[J]. 分析化学, 2019, 47(6): 941-949. doi: 10.19756/j.issn.0253-3820.191124 shu

Citation: GAO Sheng, WANG Qiao-Hua, LI Qing-Xu, SHI Hang. Non-destructive Detection of Vitamin C, Sugar Content and Total Acidity of Red Globe Grape Based on Near-Infrared Spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2019, 47(6): 941-949. doi: 10.19756/j.issn.0253-3820.191124 shu

基于近红外光谱的红提维生素C含量、糖度及总酸含量无损检测方法

高升 ^1, ,
王巧华 ^1,2, ,
李庆旭 ^1, ,
施行 ^1,

1.
华中农业大学工学院, 武汉 430070;
2.
农业部长江中下游农业装备重点实验室, 武汉 430070
基金项目:
本文系国家自然科学基金项目（No.31871863）、湖北省自然科学基金项目（No.2012FKB02910）和湖北省研究与开发计划项目（No.2011BHB016）资助

摘要: 建立了基于近红外光谱技术的红提维生素C（Vc）含量、糖度及总酸含量的快速无损检测方法。采集红提样本的光谱数据，分别应用竞争性自适应重加权算法（CARS）、稳定性竞争自适应重加权采样算法（SCARS）和连续投影算法（SPA）进行一次有效特征波段提取，对比测量上述3项指标，建立相应偏最小二乘回归模型，并在一次有效特征波段的提取的基础上结合SPA进行二次特征波段的提取，建立相应偏最小二乘回归算法（PLSR）模型。结果表明，二次特征波段建立的PLSR模型的校正集与预测集的相关系数与一次特征波段提取建立的PLSR相关系数相比有较大提高，模型的均方根误差均有所减小。根据二次特征波段提取的最优波段点建立的红提Vc含量、糖度、总酸含量的最优PLSR模型的校正集相关系数分别为0.983、0.982和0.976，预测集相关系数分别为0.975、0.980和0.975。本研究利用较少波段建立稳定模型预测Vc、糖度和总酸含量的方法，大大减少了运行时间，可为后续便携式检测仪和在线动态检测研究提供技术支持。

关键词:

English

Non-destructive Detection of Vitamin C, Sugar Content and Total Acidity of Red Globe Grape Based on Near-Infrared Spectroscopy

1.
College of Engineering, Huazhong Agricultural University, Wuhan 430070, China;
2.
Ministry of Agriculture Key Laboratory of Agricultural Equipment in the Middle and Lower Reaches of the Yangtze River, Wuhan 430070, China
Received Date: 07 March 2019
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.31871863), the Hubei Provincial Natural Science Foundation (No.2012FKB02910) and the Hubei Research and Development Program (No.2011BHB016).

Abstract: The sugar content and total acidity of red globe grape directly affect the taste and quality of fresh food taste and its by-products. Vitamin C is a necessary nutrient for human beings, and it has become the main index to evaluate the quality of red globe grape. The traditional detection method of red globe grape internal quality is destructive sampling, which is cumbersome and time consuming, and has many drawbacks. In this work, based on near-infrared spectroscopy, the rapid nondestructive detection of red globe grape Vc, sugar content and total acidity was performed. The spectral data of red globe grape samples were collected, and the competitive adaptive reweighed algorithm, stability competitive adaptive reweighed sampling algorithm and successive projection algorithm were respectively applied to extract an effective characteristic band. Then the content of Vc and sugar and the total acidity were measured comparatively, and a corresponding partial least squares regression model was established. SPA was combined to extract a secondary characteristic band on the extraction of an effective characteristic band, and a corresponding PLSR model was established. The results showed that the correlation coefficient between the correction set and the prediction set of the PLSR model established by the secondary characteristic band was higher than that established by the primary characteristic band extraction, and the root mean square error of the model was reduced. The correlation coefficients of correction set and prediction set of the optimal PLSR model for red globe grape Vc, sugar content and total acidity based on the optimal band points extracted from the secondary characteristic band were 0.983, 0.982 and 0.976, respectively, and the correlation coefficients of prediction set were 0.975, 0.980 and 0.975, respectively. This stable model built with fewer bands predicts Vc, sugar content and total acidity, and greatly reduces run time. This model provided a technical support for subsequent portable detector and online dynamic detection research.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于近红外光谱的红提维生素C含量、糖度及总酸含量无损检测方法

English

Non-destructive Detection of Vitamin C, Sugar Content and Total Acidity of Red Globe Grape Based on Near-Infrared Spectroscopy

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于近红外光谱的红提维生素C含量、糖度及总酸含量无损检测方法

English

Non-destructive Detection of Vitamin C, Sugar Content and Total Acidity of Red Globe Grape Based on Near-Infrared Spectroscopy

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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