Citation: LI Wei-Wei, WANG Shou-Chuan, SHAN Jin-Jun, DAI Qi-Gang, XU Shan, XIE Tong, LIN Li-Li, DU Li-Na, YANG Yan. Urinary Metabolomics Study of Biliary Atresia Based on Ultra-High Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(7): 1082-1089. doi: 10.19756/j.issn.0253-3820.181500 shu

Urinary Metabolomics Study of Biliary Atresia Based on Ultra-High Performance Liquid Chromatography with Linear Ion Trap-Orbitrap Mass Spectrometry

LI Wei-Wei ^1,2,3, ,
WANG Shou-Chuan ¹ ,
SHAN Jin-Jun ^2,3 ,
DAI Qi-Gang ¹ ,
XU Shan ¹ ,
XIE Tong ^2,3 ,
LIN Li-Li ^2,3 ,
DU Li-Na ⁴ ,
YANG Yan ⁴

1.
Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China;
2.
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China;
3.
Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;
4.
Beijing Children's Hospital Affiliated to Capital Medical University, Beijing 100045, China

Received Date: 1 August 2018
Revised Date: 26 January 2019

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 81473725), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (No. KYCX18_1550) and the Construction Project of National Famous Chinese Medicine Studio (No.[2018] (No.119)

A non-targeted ultra-high performance liquid chromatography with linear ion trap-orbitrap mass spectrometry (UHPLC-LTQ/Orbitrap-MS) metabolomics method was established to explore the potential urinary biomarkers of biliary atresia(BA), and to provide scientific basis for understanding the pathogenesis and early screening of BA. Urine samples from 32 BA infants and 40 normal controls were collected, and metabolic profiles of these urine samples were obtained. Finally, 32 kinds of endogenous differential metabolites were identified as potential biomarkers for BA based on principle component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Alanine, aspartic acid and glutamate, arginine and proline, D-glutamate and D-glutamine, histidine, glycine, serine and threonine metabolism pathway were involved in BA, suggesting that there was extensive disorder of amino acid metabolism in the progression of BA. Among them, glutamic acid, L-glutamine, citrulline, proline, glycocyamine, 5'-methylthioadenosine and epsilon-(gamma-Glutamyl)-lysine had obvious upward or downward trends, which are involved in a series of pathological mechanism of BA.
- Biliary atresia,
- Ultra-high performance liquid chromatography combined with linear ion trap-orbitrap mass spectrometry,
- Metabolomics,
- Urine,
- Biomarkers
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