Citation: HAN Yi-Ye, DENG Nian, XIE Jian-Jun, LIU Cheng-Lan. Acid-assisted Dispersive Liquid-Liquid Microextraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry for Determination of Mycotoxins in Fruit Juice[J]. Chinese Journal of Analytical Chemistry, ;2019, 47(3): 455-462. doi: 10.19756/j.issn.0253-3820.181299 shu

Acid-assisted Dispersive Liquid-Liquid Microextraction-High Performance Liquid Chromatography-Tandem Mass Spectrometry for Determination of Mycotoxins in Fruit Juice

1.
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education/Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province/College of Agriculture, South China Agricultural University, Guangzhou 510642, China;
2.
Guangdong Entry-Exit Inspection and Quarantine Technology Center, Guangzhou 510623, China

Received Date: 6 May 2018
Revised Date: 2 January 2019

Fund Project: This work was supported by the Project of Science and Technology of Guangdong Province (No. 2016A040403102).

A simple and novel sample pretreatment method for determination of eight kinds of mycotoxin in juice samples has been developed using acid-assisted dispersive liquid-liquid microextraction coupled with high performance liquid chromatography-tandem mass spectrometry. The factors effecting extraction efficiency were investigated including the type and volume of extraction solvent, the volume of dispersive solvent and pH of the equilibrium system. Under the optimum extraction conditions including 60 μL of trichloroaniline as extraction solvent, 1500 μL of hydrochloric acid solution as dispersive solvent and pH 5 as the acidity of equilibrium system, the method showed a good linearity for these mycotoxins within the concentration range from 1 to 500 μg/L with correlation coefficients higher than 0.994. The detection limits were 0.15-17.71 ng/L, 0.09-7.08 ng/L and 0.40-14.29 ng/L in fruit juice of mango, pineapple and dragon, respectively. The average recoveries of eight kinds of mycotoxins ranged from 75.6% to 110.0% at three spiked concentrations of 0.2 μg/L, 1.0 μg/L and 10.0 μg/L, with the relative standard deviation of lower than 15.5%. The proposed method is simple, fast with less consumption of organic solvent, and has high sensitivity and repeatability, which is suitable for the detection of mycotoxins residues in fruit juice.
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沈阳化工大学材料科学与工程学院沈阳 110142