Citation: WANG Sen, LI Bo-Wen, JIANG Long-Guang, WANG Shi-Hua, ZHANG Yan-Feng, WANG Yu. Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase (NDK) from Aspergillus Flavus[J]. Chinese Journal of Structural Chemistry, ;2016, 35(11): 1708-1713. doi: 10.14102/j.cnki.0254-5861.2011-1278 shu

Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase (NDK) from Aspergillus Flavus

1.
a Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2.
b State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China

Received Date: 12 May 2016
Available Online: 20 July 2016
Fund Project: This work was supported by National 973 Program (No. 2013CB127802) of Ministry of Science and Technology of China, and the National Natural Science Foundation of China (Nos. 31172297 and 31400100) (No. 2013CB127802)

Aspergillus flavus causes serious disease on important agriculture crops, and its secondary metabolic products-aflatoxins are most potent toxin and carcinogen for animal and human. Structural and functional studies of A. flavus proteins may provide insights into the identification of potential therapeutic targets and prevention of damage caused by A. flavus. Here, we report the expression, purification, crystallization and preliminary crystallographic analysis of NDK protein from A. flavus. The NDK protein was expressed in E. coli and purified by using a series of chromatographic methods to >98% purity. The recombinant protein was crystallized and the crystals diffracted to 2.4 Å resolution. The crystal of NDK is in space group of C121 with a=190.84, b=169.47, c=146.94 Å. Preliminary data analysis indicated that the NDK molecule assembles into a multimer in the asymmetric unit.
- Aspergillus flavus,
- nucleoside diphosphate kinase,
- expression,
- purification,
- crystallization
1. [1]
  Kai Song ZHOU , Jun Feng PENG , Ning CHANG , Han Xing ZHANG , Feng GONG , Chang Kai ZHANG . Purification and Crystallization of Flammulin,a Basic Protein with Anti-tumor Activities from Flammulina Velutipes. Chinese Chemical Letters, 2003, 14(7): 713-716.
2. [2]
  Ye Hua SHEN , Qi Dong ZHANG , Xin Du GENG , Zhi Qing ZHANG , Yun De HOU . Purification of RhlFN-in Bacteria Bodies with Acetic Acid-Water as Mobile Phase in RPLC. Chinese Chemical Letters, 2000, 11(9): 799-802.
3. [3]
  Chao Zhan WANG , Jiang Feng LIU , Xin Du GENG . Refolding with Simultaneous Purification of Recombinant Human Granulocyte Colony-stimulating Factor from Escherichia coli Using Strong Anion Exchange Chromatography. Chinese Chemical Letters, 2005, 16(3): 389-392.
4. [4]
  Ying ZHANG , Yong Lai LU , De Yan SHEN . Role of the Confined Geometry on the Crystallization of Poly(ethylene terephthlate) Ultrathin Films. Chinese Chemical Letters, 2005, 16(7): 987-990.
5. [5]
  JIA Guo-Feng , LI Fa-Qiang , PENG Zheng-Jun , ZHU Zeng-Hu , GONG Yan , WANG Qing-Lei . Synthesis and Structural Characterizations of a New Lithium Salt for Lithium-ion Batteries. Chinese Journal of Structural Chemistry, 2015, 34(8): 1197-1202. doi: 10.14102/j.cnki.0254-5861.2011-0646
6. [6]
  Li Fang TONG , Ye SHEN , Qiang ZHENG , Yong Gang SHANGGUAN . A Novel Approach for Observing Morphology of Polypropylene under Ultrasonic Vibration by SALS. Chinese Chemical Letters, 2004, 15(7): 841-844.
7. [7]
  , Diobel STEWART , Lourdes ZUMALACÁRREGUI , Daniel AMARO . Comparison among three anion exchange chromatographic supports to capture erythropoietin from cell culture supernatant Lourdes. Chinese Journal of Chromatography, 2015, 33(6): 642-646. doi: 10.3724/SP.J.1123.2015.01026
8. [8]
  Liang Quan SHENG , Shao Min LIU , Hou Rong XIAO , Bing Le XIA , Qing Liang LIU . A Novel Isoenzyme of CuZn-superoxide Dismutase from Nicotiana tobacum. Chinese Chemical Letters, 2004, 15(5): 543-546.
9. [9]
  TANG Jun-Jie , LIU Yan , TIAN Lei , WANG Dong-Xing , ZHANG Ting-An . Influence of Flow Field Distribution on the Crystallization of Spherical Nickel Hydroxide in Reactor. Chinese Journal of Inorganic Chemistry, 2016, 32(7): 1127-1134. doi: 10.11862/CJIC.2016.156
10. [10]
  ZHANG Tianyong , YANG Yuqiu , LI Bin , WANG Zhichao , ZHANG Guoling , CHEN Song , AN Jing . Catalytic Synthesis and Purification of Bisphenol S by the Products of Converted Disodium Naphthalenedisulfonate. Chinese Journal of Applied Chemistry, 2017, 34(5): 550-556. doi: 10.11944/j.issn.1000-0518.2017.05.160331
11. [11]
  HUANG Yu , REN Haowei , LIU Biao , LIU Ning , LI Meng , WANG Dongmao . Separation and identification of β-casein from Chinese human milk by ion exchange chromatography-matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry. Chinese Journal of Chromatography, 2013, 31(5): 429-434. doi: 10.3724/SP.J.1123.2013.01010
12. [12]
  LIU Zhi-Hong , YU Yan-Yan . Discussion on Problems about Purification of Crude Salt. Chinese Journal of Chemical Education, 2015, 36(4): 68-70. doi: 10.13884/j.1003-3807hxjy.2014060007
13. [13]
  LI Xia-Lan , WEI Guo-Dong , WANG Zhao-Jing , CAI E-Na , CHEN Pei-Qing . Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali. Chinese Journal of Applied Chemistry, 2006, 23(11): 1304-1306.
14. [14]
  LIAO Minfu , LIN Cuiwu , HUANG Li , XU Zijing , QIN Sasa , LI Aiyuan . Isolation, Purification and Hemostatic Activity of Water-soluble Polysaccharide in Blumea Riparia(BI.) DC.. Chinese Journal of Applied Chemistry, 2011, 28(1): 83-87. doi: 10.3724/SP.J.1095.2011.00124
15. [15]
  CUI Yuhua , YANG Jinhong , CUI Yu , SUN Guoxin , SU Benyu . Synthesis and Characterization of High Purity Polyamidoamine Dendrimers. Chinese Journal of Applied Chemistry, 2012, 29(5): 498-503. doi: 10.3724/SP.J.1095.2012.00273
16. [16]
  LI Xia , XIONG Yuan-Fu , WEN Zhu-You , JIANG Li-Hua , ZHU Hong-Fu . Purification of Lycorine from Lycoris Herb. by Macroporous Adsorbent Resin. Chinese Journal of Applied Chemistry, 2009, 26(3): 325-328.
17. [17]
  Chen Jing , Jiao Rongzhou , Zhu Yongjun . Purification and Properties of Cyanex 301. Chinese Journal of Applied Chemistry, 1996, 13(2): 45-48.
18. [18]
  Yan Yan , Zheng Goudong , Fang Chiguang , Gou Ying , Li Shaomin , Duan Weiliang . Purification of γ-Linolenic Acid from Seed Oil of Borage. Chinese Journal of Applied Chemistry, 1996, 13(6): 64-66.
19. [19]
  LI Huajun , CHEN Qian . Separation and purification of iopamidol using preparative high-performance liquid chromatography. Chinese Journal of Chromatography, 2018, 36(10): 1061-1066. doi: 10.3724/SP.J.1123.2018.06001
20. [20]
  LI Shi-ying , ZHU Xie-fei , ZHANG Li-qiang , ZHU Xi-feng . Atmospheric distillation of bio-oil based on different extractants. Journal of Fuel Chemistry and Technology, 2019, 47(3): 312-317.

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(1506)
HTML views(5)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return