Citation: Jiayi Wu, Yaping Wang, Jijun Jiang, Mei Pan, Chengyong Su. Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example[J]. University Chemistry, ;2026, 41(6): 166-174. doi: 10.12461/PKU.DXHX202605044 shu

Case design of the Chemistry “101 Plan” comprehensive chemistry experiment: taking laser confocal cell imaging of MOC-16 as an example

Jiayi Wu ¹ ,
Yaping Wang ² ,
Jijun Jiang ¹ ,
Mei Pan ^1,, ,
Chengyong Su ^1,,

1.
School of Chemistry, Sun Yat-sen University, Guangzhou 510006, Guangdong Province, China;
2.
Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510030, Guangdong Province, China

Corresponding author: Mei Pan, Chengyong Su,
Received Date: 13 May 2026
Revised Date: 21 May 2026

To meet the requirements of the Chemistry“101 Plan” for a “high-level, innovative, and challenging” comprehensive chemistry experiment course, this paper presents a comprehensive cell imaging experiment based on the biological imaging performance of pH-responsive organometallic supramolecular cage MOC-16 leveraging a large-scale instrument sharing platform. This experiment covers the complete scientific research chain including cell culture, pH-dependent cell membrane targeted incubation, one-/two-photon imaging via confocal laser scanning microscopy and image analysis, which can cultivate students’ practical ability to operate large-scale instruments and interdisciplinary thinking. As the core module of the “Modern Chemical Research Methods and Experiments” course under the chemistry “Strong Foundation Plan”, this experiment has been included in the comprehensive part of the synthetic chemistry experiment textbook of the Chemistry “101 Plan”. It can be offered as a standalone course in chemical biology experiments, or combined with the content of the synthesis and characterization of supramolecular cages to be offered as a comprehensive experiment for biomedical applications. It has refined an experimental teaching model that aligns with the goal of cultivating top-notch innovative talents and has also provided a replicable and scalable model for supporting the cultivation of basic disciplines through a large-scale instrument sharing platform.
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
  Y. Wang, K. Wu, M. Pan, K. Li, J. Mo, X. Duan, H. He, J. Shen, C. Su, ACS Appl. Mater. Interfaces 2020, 12(32), 35873.
7. [7]
  W. Li, J. Liang, Y. Wang, Y. Lu, S. Guan, X. Yan, M. Pan, ACS Appl. Mater. Interfaces 2025, 17(47), 64260.
8. [8]
  J. Fillaut, Coordin. Chem. Rev. 2024, 518, 216050.
9. [9]
  V. Juvekar, D.J. Lee, T.G. Park, R. Samanta, P. Kasar, C. Kim, F. Rotermund, H.M. Kim, Coordin. Chem. Rev. 2024, 506, 215711.
1. [1]
  Wan Li , Simin Fang , Haozhe Zha , Amirabbas Mosallanezhad , Juncheng Chen . Bilingual Instruction in First-Year Fundamental Chemistry Laboratories to Support Top Talents Development. University Chemistry, 2026, 41(4): 23-29. doi: 10.12461/PKU.DXHX202505055
2. [2]
  Qian Peng , Pengfei Yao , Zicong Wang , Xiufang Xu , Hongwei Sun . Promote the Training of Top Talents by Optimizing the Theoretical Computational Chemistry Curriculum System. University Chemistry, 2025, 40(5): 261-267. doi: 10.12461/PKU.DXHX202408012
3. [3]
  Ying Zhu , Dongliang Tian , Ting Wang , Mingjie Liu . Integrating fundamentals, frontiers, and applications in basic chemistry education: a case study on hydrogen bond structures for cultivating top science and engineering talent. University Chemistry, 2026, 41(6): 35-39. doi: 10.12461/PKU.DXHX202509074
4. [4]
  Qin Kuang , Cheng Wang , Yuanzhi Tan , Zhiyuan Jiang , Yaxian Zhu . Case-Driven Teaching Innovation and Practice of the Inorganic Synthetic Chemistry Module. University Chemistry, 2026, 41(6): 5-12. doi: 10.12461/PKU.DXHX202512009
5. [5]
  Wei Li , Jinfan Xu , Yongjun Zhang , Ying Guan . 共价有机框架整体材料的制备及食品安全非靶向筛查应用——推荐一个仪器分析综合化学实验. University Chemistry, 2025, 40(6): 276-285. doi: 10.12461/PKU.DXHX202406013
6. [6]
  Bin Liu . Teaching case design of chemistry “101 Plan” syntheticchemistry experiment course: taking the experiment of preparation of platinum nanocatalyst supported on carbon and determination of its electrochemical catalytic performance of methanol oxidation as an example. University Chemistry, 2026, 41(6): 125-135. doi: 10.12461/PKU.DXHX202506043
7. [7]
  Qingfeng Zhang , Shang-E Wei , Hua Hou , Xuan Zhao , Zixuan Yang , Lin Zhuang . Construction and Reform of the Structural Chemistry Curriculum and Textbooks under the Chemistry “101 Plan”: an In-Depth Exploration for Cultivating Top-Notch Innovative Talents. University Chemistry, 2024, 39(10): 38-44. doi: 10.12461/PKU.DXHX202409047
8. [8]
  Laiying Zhang , Weitai Wu . Design and construction of the polymer physics experiment module in the Chemistry “101 Plan” for chemistry measurement experiments. University Chemistry, 2026, 41(6): 185-195. doi: 10.12461/PKU.DXHX202512040
9. [9]
  Cunming Yu , Dongliang Tian , Jing Chen , Qinglin Yang , Kesong Liu , Lei Jiang . Chemistry “101 Program” Synthetic Chemistry Experiment Course Construction: Synthesis and Properties of Bioinspired Superhydrophobic Functional Materials. University Chemistry, 2024, 39(10): 101-106. doi: 10.12461/PKU.DXHX202408008
10. [10]
  Xianggui Kong , Wenying Shi . Comprehensive Chemical Experimental Design of Optically Encrypted Materials. University Chemistry, 2025, 40(3): 355-362. doi: 10.12461/PKU.DXHX202406067
11. [11]
  Jia Yao , Xiaogang Peng . Theory of Macroscopic Molecular Systems: Theoretical Framework of the Physical Chemistry Course in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 27-37. doi: 10.12461/PKU.DXHX202408117
12. [12]
  Yu Shen , Ting Pan , Wenyong Lai . Construction and practice of an interdisciplinary innovative closed-loop talent cultivation model under the chemistry “101 plan”: a case study of integrating material chemistry with flexible electronics. University Chemistry, 2026, 41(6): 108-117. doi: 10.12461/PKU.DXHX202511208
13. [13]
  Yunqiang Sun , Xiaowei Hu , Tingting He , Hongjiao Nie , Qiying Xia . 基于创新能力培养的综合化学实验教学改革与实践. University Chemistry, 2026, 41(10): 90-97. doi: 10.12461/PKU.DXHX202510091
14. [14]
  Guang Huang , Lei Li , Dingyi Zhang , Xingze Wang , Yugai Huang , Wenhui Liang , Zhifen Guo , Wenmei Jiao . Cobalt’s Valor, Nickel’s Foe: A Comprehensive Chemical Experiment Utilizing a Cobalt-based Imidazolate Framework for Nickel Ion Removal. University Chemistry, 2024, 39(8): 174-183. doi: 10.3866/PKU.DXHX202311051
15. [15]
  Hongyun Liu , Jiarun Li , Xinyi Li , Zhe Liu , Jiaxuan Li , Cong Xiao . Course Ideological and Political Design of a Comprehensive Chemistry Experiment: Constructing a Visual Molecular Logic System Based on Intelligent Hydrogel Film Electrodes. University Chemistry, 2024, 39(2): 227-233. doi: 10.3866/PKU.DXHX202309070
16. [16]
  Pan Li , Huguo Shen , Cong Hua , Jinjie Fang , Xiangying Chi , Quan Jiang , Zichen Feng , Ye Kang , Bin Zheng . Synthesis and Characterization of an Aggregation-Induced Emission-Active Organic Cage Molecule: A Proposed Comprehensive Chemistry Experiment. University Chemistry, 2025, 40(11): 337-345. doi: 10.12461/PKU.DXHX202502083
17. [17]
  Jinyi Sun , Lin Ma , Yanjie Xi , Jing Wang . Preparation and Electrocatalytic Nitrogen Reduction Performance Study of Vanadium Nitride@Nitrogen-Doped Carbon Composite Nanomaterials: A Recommended Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(4): 184-191. doi: 10.3866/PKU.DXHX202310094
18. [18]
  Xin Zhou , Zhi Zhang , Yun Yang , Shuijin Yang . A Study on the Enhancement of Photocatalytic Performance in C/Bi/Bi₂MoO₆ Composites by Ferroelectric Polarization: A Recommended Comprehensive Chemical Experiment. University Chemistry, 2024, 39(4): 296-304. doi: 10.3866/PKU.DXHX202310008
19. [19]
  Guanghui Wang , Chen Qian , Zhiyong Ma . Preparation and Characterization of 7H-Benzo[C]Carbazole Based Ultra-Long Organic Room Temperature Phosphorescence Material. University Chemistry, 2025, 40(11): 289-299. doi: 10.12461/PKU.DXHX202412062
20. [20]
  Wen-Bo Liu . Less is more: a teaching perspective on organometallic chemistry for the top-notch undergraduate training program in chemistry. University Chemistry, 2026, 41(6): 60-63. doi: 10.12461/PKU.DXHX202509131

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(540)
HTML views(77)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142