Citation: Ruilin He, Fengyi Wang, Zhikai Xia, Linling Zheng, Yue Liu. 球核酸的制备及DNA检测应用[J]. University Chemistry, ;2026, 41(5): 230-238. doi: 10.12461/PKU.DXHX202511108 shu

球核酸的制备及DNA检测应用

College of Basic Medical Sciences, Army Medical University, Chongqing 400038, China

Corresponding author: Linling Zheng, Yue Liu,
Received Date: 20 November 2025
Revised Date: 26 February 2026

溶胶是生物医学领域常用的纳米材料，其在生物传感、生物成像、生物医药等方面有广泛的应用。在现有溶胶基本性质研究实验基础上，进一步开展溶胶的修饰和应用实验对拓展医学生的溶胶前沿知识、深化理解溶胶的医学应用具有重要意义。球核酸是溶胶修饰和应用最为成熟和典型的代表。本文结合团队关于球核酸制备的创新研究成果，面向医学检验技术专业新创球核酸的制备及应用实验。该实验系统融合金溶胶的合成、球核酸的制备(即金溶胶的DNA修饰)和DNA检测应用，实验内容前沿新颖，且设计合理、层层递进，有利于激发学生实验探究兴趣。实验结果表明，该新创实验操作简单、无需大型仪器、绿色安全、可行性和重复性好，适合本科生实验教学。本实验项目以培养科学创新素养为导向，将理论知识与实际问题相结合，且具备一定的挑战度，有助于提升学生综合运用知识分析问题、解决问题的能力。
- Gold sol,
- Spherical nucleic acid,
- Preparation,
- Characterization,
- DNA detection
1. [1]
2. [2]
  J.I. Cutler, E. Auyeung, C.A. Mirkin, J. Am. Chem. Soc. 2012, 134 (3), 1376.
3. [3]
  R. Elghanian, J.J. Storhoff, R.C. Mucic, R.L. Letsinger, C.A. Mirkin, Science 1997, 277 (5329), 1078.
4. [4]
  K. Saha, S.S. Agasti, C. Kim, X. Li, V.M. Rotello, Chem. Rev. 2012, 112 (5), 2739.
5. [5]
  W. Zhou, X. Gao, D. Liu, X. Chen, Chem. Rev. 2015, 115 (19), 10575.
6. [6]
  K. Jiang, J. Wu, J.-E. Kim, S. An, J.-M. Nam, Y.-K. Peng, J.-H. Lee, Nano Lett. 2023, 23 (9), 3897.
7. [7]
  R. Yuan, W. Sha, W. Xie, G. Qu, H. Zhang, H. Wang, X.C. Le, J. Am. Chem. Soc. 2024, 146 (39), 26657.
8. [8]
  X. Zhong, J. Hua, M. Shi, Y. He, Y. Huang, B. Wang, L. Zhang, S. Zhao, L. Hou, ACS Sens. 2024, 9 (3), 1280.
9. [9]
  M.-E. Kyriazi, D. Giust, A.H. El-Sagheer, P.M. Lackie, O.L. Muskens, T. Brown, ACS Nano 2018, 12 (4), 3333.
10. [10]
  J. Li, X. Mao, T. Zhao, W. Fang, Y. Jin, M. Liu, C. Fan, Y. Tian, Angew. Chem. Int. Ed. 2024, 64 (5), e202416988.
11. [11]
  J. Liu, Y. Lu, Nat. Protoc. 2006, 1 (1), 246.
12. [12]
  X. Zhang, M.R. Servos, J. Liu, J. Am. Chem. Soc. 2012, 134 (17), 7266.
13. [13]
  B. Liu, J. Liu, J. Am. Chem. Soc. 2017, 139 (28), 9471.
14. [14]
  Y. Hao, Y. Li, L. Song, Z. Deng, J. Am. Chem. Soc. 2021, 143 (8), 3065.
15. [15]
  M. Huang, E. Xiong, Y. Wang, M. Hu, H. Yue, T. Tian, D. Zhu, H. Liu, X. Zhou, Nat. Commun. 2022, 13 (1), 968.
16. [16]
  Y. Ye, Y. Hao, M. Ye, X. Song, Z. Deng, Small 2022, 18 (24), 2202458.
17. [17]
  Z. Ding, H. Gao, C. Wang, Y. Li, N. Li, L. Chu, H. Chen, H. Xie, Angew. Chem. Int. Ed. 2024, 63 (20), e202317463.
18. [18]
  M.R. Green, J. Sambrook, Cold Spring Harb. Protoc. 2016, 2016 (12), pdb.prot093377.
1. [1]
  Lingqi Zhang , Hairong Huang , Jialin Li , Li Ji , Yufan Pan , Meiling Ye , Cuixue Chen , Shunü Peng . 桂花碳量子点的绿色制备及科普应用方案. University Chemistry, 2025, 40(8): 298-306. doi: 10.12461/PKU.DXHX202409138
2. [2]
  Yongming Guo , Jie Li , Chaoyong Liu . Green Improvement and Educational Design in the Synthesis and Characterization of Silver Nanoparticles. University Chemistry, 2024, 39(3): 258-265. doi: 10.3866/PKU.DXHX202309057
3. [3]
  Qiuping Liu , Yongxian Fan , Wenxian Chen , Mengdi Wang , Mei Mei , Genrong Qiang . Design of Ideological and Political Education for the Preparation Experiment of Ferrous Sulfate. University Chemistry, 2024, 39(2): 116-120. doi: 10.3866/PKU.DXHX202309083
4. [4]
  Wenyu Yuan , Ying Wang , Shuni Li , Xiaolin Zhu , Quanguo Zhai , Shengli Gao . Preparation and Purification of Alkali Metals. University Chemistry, 2026, 41(2): 218-231. doi: 10.12461/PKU.DXHX202502111
5. [5]
  Yufan Pan , Xue Ding , Jiayu Lin , Haiting Wu , Hairong Huang , Cuixue Chen , Meiling Ye . Oil Cosmetics, Charming Chemistry: A Gradient Science Popularization Scheme for Cream Cosmetic Preparation. University Chemistry, 2025, 40(4): 382-389. doi: 10.12461/PKU.DXHX202406078
6. [6]
  Xinzhe HUANG , Lihui XU , Yue YANG , Liming WANG , Zhangyong LIU , Zhongjian WANG . Preparation and visible light responsive photocatalytic properties of BiSbO₄/BiOBr. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 284-292. doi: 10.11862/CJIC.20240212
7. [7]
  Haiyu Nie , Chenhui Zhang , Fengpei Du . Ideological and Political Design for the Preparation, Characterization and Particle Size Control Experiment of Nanoemulsion. University Chemistry, 2024, 39(2): 41-46. doi: 10.3866/PKU.DXHX202306055
8. [8]
  Lei Shu , Zhengqing Hao , Kai Yan , Hong Wang , Lihua Zhu , Fang Chen , Nan Wang . Development of a Double-Carbon Related Experiment: Preparation, Characterization and Carbon-Capture Ability of Eggshell-Derived CaO. University Chemistry, 2024, 39(4): 149-156. doi: 10.3866/PKU.DXHX202310134
9. [9]
  Keweiyang Zhang , Zihan Fan , Liyuan Xiao , Haitao Long , Jing Jing . Unveiling Crystal Field Theory: Preparation, Characterization, and Performance Assessment of Nickel Macrocyclic Complexes. University Chemistry, 2024, 39(5): 163-171. doi: 10.3866/PKU.DXHX202310084
10. [10]
  Xinyuan Shi , Chenyangjiang , Changyu Zhai , Xuemei Lu , Jia Li , Zhu Mao . Preparation and Photoelectric Performance Characterization of Perovskite CsPbBr₃ Thin Films. University Chemistry, 2024, 39(6): 383-389. doi: 10.3866/PKU.DXHX202312019
11. [11]
  Cong Liu , Yuling Huang , Peng Zhen , Hualiu Li , Bingjia Xu , Suilian Luo , Guang Shi . A Comprehensive Design Experiment: Preparation and Property Characterization of Polymer Room-Temperature Phosphorescence Materials. University Chemistry, 2026, 41(4): 409-416. doi: 10.12461/PKU.DXHX202503092
12. [12]
  Zunyuan Xie , Lijin Yang , Zixiao Wan , Xiaoyu Liu , Yushan He . Exploration of the Preparation and Characterization of Nano Barium Titanate and Its Application in Inorganic Chemistry Laboratory Teaching. University Chemistry, 2024, 39(4): 62-69. doi: 10.3866/PKU.DXHX202310137
13. [13]
  Yixuan Gao , Lingxing Zan , Wenlin Zhang , Qingbo Wei . Comprehensive Innovation Experiment: Preparation and Characterization of Carbon-based Perovskite Solar Cells. University Chemistry, 2024, 39(4): 178-183. doi: 10.3866/PKU.DXHX202311091
14. [14]
  Kai Yang , Gehua Bi , Yong Zhang , Delin Jin , Ziwei Xu , Qian Wang , Lingbao Xing . Comprehensive Polymer Chemistry Experiment Design: Preparation and Characterization of Rigid Polyurethane Foam Materials. University Chemistry, 2024, 39(4): 206-212. doi: 10.3866/PKU.DXHX202308045
15. [15]
  Jinfeng Chu , Yicheng Wang , Ji Qi , Yulin Liu , Yan Li , Lan Jin , Lei He , Yufei Song . Comprehensive Chemical Experiment Design: Convenient Preparation and Characterization of an Oxygen-Bridged Trinuclear Iron(III) Complex. University Chemistry, 2024, 39(7): 299-306. doi: 10.3866/PKU.DXHX202310105
16. [16]
  Juan Yuan , Bin Zhang , Jinping Wu , Mengfan Wang . Design of a Comprehensive Experiment on Preparation and Characterization of Cu₂(Salen)₂ Nanomaterials with Two Distinct Morphologies. University Chemistry, 2024, 39(10): 420-425. doi: 10.3866/PKU.DXHX202402014
17. [17]
  Feng Lu , Tao Wang , Qi Wang . Preparation and Characterization of Water-Soluble Silver Nanoclusters: A New Design and Teaching Practice in Materials Chemistry Experiment. University Chemistry, 2025, 40(4): 375-381. doi: 10.12461/PKU.DXHX202406005
18. [18]
  Qinqi Zhang , Zhuoran Bi , Yi Zhong , Ziting Xu , Lanlan Chen . 双重信号放大功能核酸荧光探针用于水中抗生素的超灵敏检测. University Chemistry, 2026, 41(5): 448-454. doi: 10.12461/PKU.DXHX202511151
19. [19]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
20. [20]
  Kun JIANG , Yutong XUE , Kelin LIU , Miao WANG , Tongming SUN , Yanfeng TANG . CeVO₄ hollow microspheres: Fabrication and adsorption performance for dyes. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2229-2236. doi: 10.11862/CJIC.20250223

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(115)
HTML views(21)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142