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Citation: Huairou Zhu, Qiaoyi Cen, Dan Yan, Wei Yi, Guosheng Chen, Siming Huang, Gangfeng Ouyang. 基于仿生矿化酶固定纳米探针对血清葡萄糖定量检测的实验新探索[J]. University Chemistry, ;2026, 41(5): 330-339. doi: 10.12461/PKU.DXHX202511041 shu

基于仿生矿化酶固定纳米探针对血清葡萄糖定量检测的实验新探索

1.
School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436, Guangdong Province, China;
2.
School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, Guangdong Province, China

Corresponding author: Siming Huang, huangsm@gzhmu.edu.cn
Received Date: 6 November 2025
Revised Date: 26 February 2026

血清葡萄糖检测是临床生物化学检验技术实验课程中的经典实验，但现有实验存在色原底物毒性较高、方法抗干扰能力弱和仪器检测耗时较长等问题。本改进实验延续了酶级联催化的核心原理，从色原底物优化、纳米探针合成和检测仪器升级三个方面对实验内容与教学方式进行改进：1) 采用低毒性2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)替代高毒性4-氨基安替比林色原底物，从而显著提高实验安全性；2) 通过仿生酶固定化技术制备稳定且灵敏的纳米探针，有效增强方法的抗干扰能力和检测准确性，同时提升实验的创新性与高阶性；3) 使用高通量酶标仪取代紫外-可见分光光度计作为定量检测仪器，大幅缩短分析时长并减少样本消耗。教学实践表明，该纳米探针具有合成简便、成本低、重现性好及检测准确度优异等特点。本改进实验实现了教学安全性、高阶性与前沿性的有机统一，其模块化的实验流程可保证实验在7-10学时内完成，为临床生物化学类实验课程改革提供了可推广的范例。
- Serum glucose,
- Enzyme immobilization,
- Nanoprobe,
- Colorimetric analysis,
- Experimental improvement
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