Citation: Yubin Su, Chenyu Yao, Shuyan Chen, Lisha Xu, Min Peng, Yawen Wang, Yu Peng, Jianfeng Zheng. 一种聚集诱导发光荧光探针的合成及其在指纹显影中的应用——本科生综合性化学实验探索[J]. University Chemistry, ;2026, 41(5): 70-78. doi: 10.12461/PKU.DXHX202511031 shu

一种聚集诱导发光荧光探针的合成及其在指纹显影中的应用——本科生综合性化学实验探索

School of Chemistry, Southwest Jiaotong University, Chengdu 610031, Sichuan Province, China

Corresponding author: Yawen Wang, Yu Peng,
Received Date: 5 November 2025
Revised Date: 25 February 2026

聚集诱导发光(AIE)材料作为荧光传感与生物成像领域的前沿体系，将其引入本科实验教学对培养化学创新人才具有重要的教学价值。本实验设计并合成了一种基于四苯基乙烯(TPE)骨架的AIE荧光探针TPE-Th-1-OH。通过密度泛函理论(DFT)计算筛选出目标分子，采用Knoevenagel缩合反应合成，并通过核磁共振氢谱与熔点测试确证结构，系统训练学生在理论计算、有机合成与结构表征方面的综合实践能力。光物理性能研究结果表明，该探针在较宽的pH范围(3.0-10.0)内具有良好的荧光稳定性(强度波动 < 10%)。将探针应用于多种基底(硬卡纸、椴木板、氧化铝陶瓷、亚克力板、盖玻片、铝箔)上的指纹显影，均展现出高分辨率与强抗背景干扰能力，有效激发学生对化学与实际应用相结合的学习兴趣。本实验将AIE前沿理论与本科实验教学深度融合，涵盖计算化学、有机合成、光谱表征与功能应用四大关键模块，构建了“理论-合成-表征-应用”一体化的综合性化学实验教学体系，有助于系统提升高年级本科生的综合分析能力与科研创新能力。
- Aggregation-induced emission,
- Fluorescent probe,
- Tetraphenylethylene derivative,
- Fingerprint development,
- Comprehensive chemistry experiment
1. [1]
  J. Luo, Z. Xie, J.W.Y. Lam, L. Cheng, H. Chen, C. Qiu, H.S. Kwok, X. Zhan, Y. Liu, D. Zhu, et al., Chem. Commun. 2001, 18, 1740.
2. [2]
  J. Mei, N.L.C. Leung, R.T.K. Kwok, J.W.Y. Lam, B.Z. Tang, Chem. Rev. 2015, 115, 11718.
3. [3]
  D. Yan, Q. Wu, D. Wang, B.Z. Tang, Angew. Chem. Int. Ed. 2018, 60, 15724.
4. [4]
  Y. Hong, J.W.Y. Lam, B.Z. Tang, Chem. Soc. Rev. 2011, 40, 5361.
5. [5]
  J. Ni, Q. Zhang, H. Zhang, Z.J. Sun, B.Z. Tang, Sci. China Mater. 2023, 66, 1959.
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
  J.K. Liu, H.K. Zhang, L.R. Hu, J. Wang, J.W.Y. Lam, L. Blancafort, B.Z. Tang, J. Am. Chem. Soc. 2022, 144, 7901.
11. [11]
  F. Han, L. Liu, Spectrochim. Acta A Mol. Biomol. Spectrosc. 2022, 281, 121617.
12. [12]
  Y.L. Wang, C. Li, H.Q. Qu, C. Fan, P.J. Zhao, R. Tian, M.Q. Zhu, J. Am. Chem. Soc. 2020, 142, 7497.
13. [13]
1. [1]
  Xinrong Wu , Yingying Ren , Jianxue Wang , Lijin Yang , Jia Jia , Nan Li , Na Zhao . 聚光捕胺——高灵敏胺响应型聚集诱导发光探针的合成及传感应用. University Chemistry, 2026, 41(5): 180-189. doi: 10.12461/PKU.DXHX202509129
2. [2]
  Senqiang Zhu , Ruohan Li , Yujia Yang , Jinzhi Liao , Rui Liu . 聚光成辉——Suzuki偶联反应高效制备聚集诱导发射荧光分子及其潜指纹识别应用. University Chemistry, 2026, 41(5): 109-119. doi: 10.12461/PKU.DXHX202511066
3. [3]
  Pan Li , Huguo Shen , Cong Hua , Jinjie Fang , Xiangying Chi , Quan Jiang , Zichen Feng , Ye Kang , Bin Zheng . Synthesis and Characterization of an Aggregation-Induced Emission-Active Organic Cage Molecule: A Proposed Comprehensive Chemistry Experiment. University Chemistry, 2025, 40(11): 337-345. doi: 10.12461/PKU.DXHX202502083
4. [4]
  Yanyang Li , Zongpei Zhang , Kai Li , Shuangquan Zang . Ideological and Political Design for the Comprehensive Experiment of the Synthesis and Aggregation-Induced Emission (AIE) Performance Study of Salicylaldehyde Schiff-Base. University Chemistry, 2024, 39(2): 105-109. doi: 10.3866/PKU.DXHX202307020
5. [5]
  Qiang HU , Zhiqi CHEN , Zhong CHEN , Xu WANG , Weina WU . Pyridinium-chalcone-based ClO^- fluorescent probe: Preparation and biological imaging applications. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1789-1795. doi: 10.11862/CJIC.20250086
6. [6]
  Ruoqian Zhang , Chaoqun Mu , Yali Hou , Mingming Zhang . 四苯乙烯基多组分金属有机笼的构筑及其固态发光性能研究. University Chemistry, 2025, 40(8): 277-283. doi: 10.12461/PKU.DXHX202410027
7. [7]
  Jun LUO , Baoshu LIU , Yunchang ZHANG , Bingkai WANG , Beibei GUO , Lan SHE , Tianheng CHEN . Europium(Ⅲ) metal-organic framework as a fluorescent probe for selectively and sensitively sensing Pb²⁺ in aqueous solution. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2438-2444. doi: 10.11862/CJIC.20240240
8. [8]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
9. [9]
  Pengli GUAN , Renhu BAI , Xiuling SUN , Bin LIU . Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging. Chinese Journal of Inorganic Chemistry, 2025, 41(9): 1817-1826. doi: 10.11862/CJIC.20250058
10. [10]
  Rui Gao , Ying Zhou , Yifan Hu , Siyuan Chen , Shouhong Xu , Qianfu Luo , Wenqing Zhang . Design, Synthesis and Performance Experiment of Novel Photoswitchable Hybrid Tetraarylethenes. University Chemistry, 2024, 39(5): 125-133. doi: 10.3866/PKU.DXHX202310050
11. [11]
  Yingpeng ZHANG , Xingxing LI , Yunshang YANG , Zhidong TENG . A pyrazole-based turn-off fluorescent probe for visual detection of hydrazine. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1301-1308. doi: 10.11862/CJIC.20250064
12. [12]
  Yuting DU , Jing YUAN , Peiyao DENG . Synthesis and application of a fluorescent probe for the detection of reduced glutathione. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1331-1337. doi: 10.11862/CJIC.20240461
13. [13]
  Hongxia Yan , Rui Wu , Weixu Feng , Yan Zhao , Yi Yan . Innovation Inspired by Classical Chemistry: Luminescent Hyperbranched Polysiloxanes. University Chemistry, 2025, 40(4): 154-159. doi: 10.12461/PKU.DXHX202409010
14. [14]
  Yanxi LIU , Mengjia XU , Haonan CHEN , Quan LIU , Yuming ZHANG . A fluorescent-colorimetric probe for peroxynitrite-anion-imaging in living cells. Chinese Journal of Inorganic Chemistry, 2025, 41(6): 1112-1122. doi: 10.11862/CJIC.20240423
15. [15]
  Shunping WANG , Chuandong GE , Shuguang QU , Tianduo LI , Shaomin WANG , Qingyuan YANG . Synthesis of a fluorescent probe based on urea-based coordination and Si—O bond cleavage for F^- and Cr(Ⅵ) detection. Chinese Journal of Inorganic Chemistry, 2026, 42(2): 297-308. doi: 10.11862/CJIC.20250214
16. [16]
  Hongxia Yan , Weixu Feng , Junyan Yao , Wei Tian , Rui Wang . Illuminating the Teaching of Science and Engineering Graduate Courses with “Curriculum Ideology and Politics”. University Chemistry, 2024, 39(6): 122-127. doi: 10.3866/PKU.DXHX202310059
17. [17]
  Yu SU , Xinlian FAN , Yao YIN , Lin WANG . From synthesis to application: Development and prospects of InP quantum dots. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2105-2123. doi: 10.11862/CJIC.20240126
18. [18]
  Xiaohong Li , Huanjiang Wang , Fang Tan , Xiaohua Cai , Yingchun Luo , Yanli Leng , Guoyong Zhou , Zhu Wen . Research on the Project-Driven Teaching Model in the Context of New Engineering: A Case Study of the Development of a “Portable Metal ions and Pesticide Residues Intelligent Detection System”. University Chemistry, 2026, 41(1): 204-212. doi: 10.12461/PKU.DXHX202510037
19. [19]
  Jia-He Li , Yu-Ze Liu , Jia-Hui Ma , Qing-Xiao Tong , Jian-Ji Zhong , Jing-Xin Jian . 洛芬碱衍生物的合成、化学发光与重金属离子检测. University Chemistry, 2025, 40(6): 230-237. doi: 10.12461/PKU.DXHX202407080
20. [20]
  Jianfeng Yan , Yating Xiao , Xin Zuo , Caixia Lin , Yaofeng Yuan . Comprehensive Chemistry Experimental Design of Ferrocenylphenyl Derivatives. University Chemistry, 2024, 39(4): 329-337. doi: 10.3866/PKU.DXHX202310005

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(97)
HTML views(19)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142