Citation: Xiuyun Wang, Faqiong Zhao, Yuwen Liu, Yijun Li, Yong Fan, Dongcheng Liu, Juanjuan Song, Yanping Ren, Wan Li, Yongxian Fan, Xiuqiong Zeng, Wenwei Zhang, Mei Shi, Min Hu, Xiaohang Qiu, Weihong Li, Jinarong Zhang, Shuyong Zhang. Spectrophotometry and Suggestions on Operating Specifications for Spectrophotometer[J]. University Chemistry, ;2026, 41(3): 200-207. doi: 10.12461/PKU.DXHX202507116 shu

Spectrophotometry and Suggestions on Operating Specifications for Spectrophotometer

Xiuyun Wang ¹ ,
Faqiong Zhao ² ,
Yuwen Liu ² ,
Yijun Li ^3,, ,
Yong Fan ⁴ ,
Dongcheng Liu ⁵ ,
Juanjuan Song ⁶ ,
Yanping Ren ^7,, ,
Wan Li ⁸ ,
Yongxian Fan ⁹ ,
Xiuqiong Zeng ¹⁰ ,
Wenwei Zhang ¹¹ ,
Mei Shi ¹² ,
Min Hu ¹³ ,
Xiaohang Qiu ³ ,
Weihong Li ¹⁴ ,
Jinarong Zhang ^11,, ,
Shuyong Zhang ^15,,

1.
National Demonstration Center for Experimental Chemistry Education (Dalian University of Technology), School of Chemistry, Dalian University of Technology, Dalian 116024, Liaoning Province, China;
2.
National Demonstration Center for Experimental Chemistry Education (Wuhan University), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;
3.
National Demonstration Center for Experimental Chemistry Education (Nankai University), College of Chemistry, Nankai University, Tianjin 300071, China;
4.
National Demonstration Center for Experimental Chemistry Education (Jilin University), College of Chemistry, Jilin University, Changchun 130012, China;
5.
National Demonstration Center for Experimental Chemistry Education (Guangxi Normal University), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, Guangxi Zhuang Autonomous Region, China;
6.
National Demonstration Center for Experimental Chemistry Education (Inner Mongolia Minzu University), College of Chemistry and Materials Science, Inner Mongolia Minzu University, Tongliao 028000, Inner Mongolia Autonomous Region, China;
7.
National Demonstration Center for Experimental Chemistry Education (Xiamen University), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China;
8.
National Demonstration Center for Experimental Chemistry Education (University of Science and Technology of China), School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China;
9.
National Demonstration Center for Experimental Chemistry and Chemical Engineering Education (Zhejiang University of Technology), College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China;
10.
National Demonstration Center for Experimental Chemistry Education (Zhejiang University), Department of Chemistry, Zhejiang University, Hangzhou 310058, China;
11.
National Demonstration Center for Experimental Chemistry Education (Nanjing University), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China;
12.
National Demonstration Center for Experimental Chemistry Education (Fudan University), Department of Chemistry, Fudan University, Shanghai 200433, China;
13.
School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, China;
14.
National Demonstration Center for Experimental Chemistry Education (Peking University), College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
15.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China

Corresponding author: Yijun Li, Yanping Ren, Jinarong Zhang, Shuyong Zhang,
Received Date: 28 July 2025
Accepted Date: 27 August 2025

Spectrophotometry is a spectroscopic analytical method based on the selective absorption of electromagnetic radiation of ultraviolet, visible, and other electromagnetic radiation by substances, used for both qualitative and quantitative analysis. It is characterized by operational simplicity, high sensitivity, rapid measurement, and wide applicability. The spectrophotometer is the instrument used to perform this method, and its proper operation is an essential experimental skill for students majoring in chemistry and related disciplines. This article mainly introduces the fundamental principles of ultraviolet-visible (UV-Vis) spectrophotometry (commonly referred to simply as spectrophotometry) and the operation of spectrophotometer, and provides suggestions on operating specifications.
- Chemical experiment,
- Spectrophotometry,
- Spectrophotometer,
- Operating standards
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