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Citation: Aijia Tang, Xinze Li, Yaning Shi, Xueen Fang. 玩“转”芯片，让DNA看得见[J]. University Chemistry, ;2026, 41(8): 276-285. doi: 10.12461/PKU.DXHX202507111 shu

玩“转”芯片，让DNA看得见

Department of Chemistry, Fudan University, Shanghai 200438, China

Corresponding author: Xueen Fang, fxech@fudan.edu.cn
Received Date: 28 July 2025
Accepted Date: 25 September 2025

基于旋转式微流控芯片技术，设计了一套操作简便、结果可视化的DNA分析实验，旨在实现让DNA“看得到、看得准、看得快”。以转基因大豆快速鉴定为例，提取非转基因和两种转基因(BPS-CV127-9、MON87701)大豆粉末的DNA，结合紫外分光光度法和荧光法进行初步鉴定。通过多色荧光鉴定DNA的方法流程简易且趣味性强，适合中小学生及学龄前儿童动手参与；构建芯片离心机、恒温驱动微流控芯片化学反应，利用沉淀法、pH指示剂法、荧光指示法观测结果，实现快速特异性DNA鉴定，适用于高中生、大学生开展科普活动和综合实验。此外，基于此实验可面向大众辟谣不科学的转基因作物鉴别方法，并科普相关科学原理与国家政策。本实验以多种有趣的形式开展梯度科普，涵盖的受众群体广泛，以期让公众体会到化学的重要性和化学之美。
- Microfluidics,
- DNA,
- Tiered science outreach,
- Genetically modified organisms (GMOs),
- Rapid detection
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