Citation: Jianna Peng, Hongjie Song, Lichun Zhang, Rui Liu, Yi Lü. Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques[J]. University Chemistry, ;2026, 41(4): 231-238. doi: 10.12461/PKU.DXHX202506070 shu

Flow Cytometry: Principles, Applications, and Innovations in Spectroscopic Techniques

Jianna Peng ^1,2 ,
Hongjie Song ¹ ,
Lichun Zhang ¹ ,
Rui Liu ^1,, ,
Yi Lü ^1,3

1.
College of Chemistry, Sichuan University, Chengdu 610064, Sichuan Province, China;
2.
College of Chemical Science and Engineering, Yunnan University, Kunming 650091, Yunnan Province, China;
3.
Analytical & Testing Center, Sichuan University, Chengdu 610064, Sichuan Province, China

Corresponding author: Rui Liu, liur@scu.edu.cn
Received Date: 7 June 2025
Accepted Date: 11 July 2025

Flow cytometry enables the detection of scattered light and fluorescence emitted by cells when illuminated vertically by a laser beam, thereby facilitating comprehensive cellular analysis. The advancements in photoelectric technology have driven it from single-parameter to multi-parameter analysis, culminating in the development of full-spectrum flow cytometry. This evolution allows for the acquisition of extensive biological information from complex samples and has a wide range of influences. The article elaborates on the historical development, principles, and diverse applications of flow cytometry, and emphasizes the innovations in spectral technology. Additionally, it outlines potential future directions for the field.
- Flow cytometry,
- Principle,
- Application,
- Spectral technology innovation,
- Full-spectrum flow cytometry
1. [1]
  S.M. Manohar, P. Shah, A. Nair, Bioanalysis 2021, 13 (3), 185.
2. [2]
  J.P. Robinson, Biotechniques 2022, 72 (4), 159.
3. [3]
  J.P. Nolan, Cytom. Part A 2022, 101 (10), 812.
4. [4]
  S. Sharma, J. Boyer, L. Teyton, Nat. Methods 2024, 21 (5), 740.
5. [5]
  J.P. Robinson, Cytom. Part A 2019, 95 (8), 823.
6. [6]
7. [7]
8. [8]
9. [9]
  A. Adan, G. Alizada, Y. Kiraz, Y. Baran, A. Nalbant, Crit. Rev. Biotechnol. 2017, 37 (2), 163.
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
  C.K. Snow, Cytom. Part A 2004, 57 (2), 63.
15. [15]
16. [16]
17. [17]
  P.K. Chattopadhyay, A.F. Winters, W.E. Lomas, A.S. Laino, D.M. Woods, Annu. Rev. Anal. Chem. 2019, 12 (1), 411.
18. [18]
  W.G. Telford, T. Hawley, F. Subach, V. Verkhusha, R.G. Hawley, Methods 2012, 57 (3), 318.
19. [19]
  D. Wlodkowic, W. Telford, J. Skommer, Z. Darzynkiewicz, Methods Cell Biol. 2011, 103, 55.
20. [20]
  V. Kapoor, F.V. Subach, V.G. Kozlov, A. Grudinin, V.V. Verkhusha, W.G. Telford, Nat. Methods 2007, 4 (9), 678.
21. [21]
  F. Reggeti, D. Bienzle, Vet. Pathol. 2011, 48 (1), 223.
22. [22]
  H.T. Maecker, J. Trotter, Cytom. Part A 2006, 69 (9), 1037.
23. [23]
24. [24]
  J.P. Nolan, D. Condello, E. Duggan, M. Naivar, D. Novo, Cytom. Part A 2013, 83 (3), 253.
25. [25]
  Y. Han, Y. Gu, A.C. Zhang, Y. Lo, Lab Chip 2016, 16 (24), 4639.
26. [26]
  M. Roederer, Cytometry 2001, 45 (3), 194.
27. [27]
28. [28]
  L. Li, S. Wang, J. Xue, Y. Lin, L. Su, C. Xue, C. Mao, N. Cai, Y. Tian, S. Zhu, et al. Anal. Chem. 2023, 95 (6), 3423.
29. [29]
  M.Y. Chang, J.E. Brune, M. Black, W.A. Altemeier, C.W. Frevert, Am. J. Physiol.-Lung Cell. Mol. Physiol. 2023, 325 (4), L518.
30. [30]
  M. Yamamoto, J. P. Robinson, Cytom. Part A 2021, 99 (2), 145.
31. [31]
  L. Michelutti, M. Bulfoni, E. Nencioni, J. Microbiol. Methods 2020, 170, 105834.
32. [32]
  A. Álvarez-Barrientos, J. Arroyo, R. Cantón, C. Nombela, M. Sánchez-Pérez, Clin. Microbiol. Rev. 2000, 13 (2), 167.
33. [33]
34. [34]
35. [35]
36. [36]
  C. Zhang, F.B. Wu, Y.Y. Zhang, X. Wang, X.R. Zhang, J. Anal. At. Spectrom. 2001, 16 (12), 1393.
37. [37]
  R. Liu, S.X. Zhang, C. Wei, Z. Xing, S.C. Zhang, X.R. Zhang, Accounts Chem. Res. 2016, 49 (5), 775.
38. [38]
  S.C. Bendall, E.F. Simonds, P. Qiu, E.D. Amir, P.O. Krutzik, R. Finck, R.V. Bruggner, R. Melamed, A. Trejo, O.I. Ornatsky, et al. Science 2011, 332 (6030), 687.
39. [39]
  N. Nitta, T. Sugimura, A. Isozaki, H. Mikami, K. Hiraki, S. Sakuma, T. Iino, F. Arai, T. Endo, Y. Fujiwaki, et al. Cell 2018, 175 (1), 266.
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沈阳化工大学材料科学与工程学院沈阳 110142