Home

Citation: Shunü Peng, Huamin Li, Zhaobin Chen, Yiru Wang. Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations[J]. University Chemistry, ;2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043 shu

Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations

National Demonstration Center for Experimental Chemistry Education (Xiamen University), College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China

Received Date: 4 December 2024
Revised Date: 22 January 2025

This study enhances an undergraduate instrumental analysis experiment focused on the separation and quantification of five principal components—eucalyptol, camphor, menthol, methyl salicylate, and eugenol—in traditional Chinese medicine preparations using gas chromatography. It introduces a simultaneous application of internal standard, external standard, and normalization methods to the same dataset to assess the accuracy of quantitative results. This approach enables students to grasp fundamental chromatographic qualitative and quantitative techniques, understand the applicability of various quantitative methods, and learn to calculate theoretical plate numbers and resolution within a limited teaching timeframe. By integrating basic chromatographic theory with the analysis of main components in everyday items such as wind oil essence, this experiment not only demonstrates the practical application of theoretical knowledge but also promotes environmental sustainability. It effectively cultivates students' capabilities in analyzing experimental data.
- Gas chromatography,
- Wind oil essence,
- Instrumental analysis experiment,
- Qualitative and quantitative methods
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
1. [1]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
2. [2]
  Zelin Wang , Gang Liu , Mengran Wang , Peiyu Zhang , Aixin Song , Jingcheng Hao , Jiwei Cui . Application of Instrumental Analysis in the Detection of Organic Components in Liquor. University Chemistry, 2025, 40(11): 318-326. doi: 10.12461/PKU.DXHX202502077
3. [3]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
4. [4]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
5. [5]
  Yifan Xie , Liyun Yao , Ruolin Yang , Yuxing Cai , Yujie Jin , Ning Li . Application of Comparative Pedagogy in Instrumental Analysis Experiment Teaching. University Chemistry, 2024, 39(3): 266-273. doi: 10.3866/PKU.DXHX202309068
6. [6]
  Zisheng Xiao , Siyi Liu , Binhong He , Yi Xiao , Qiong Xu , Zhili Lan , Rong Tan , Liang Tan , Dulin Yin . Construction and Practice of Instrumental Analysis Experimental Teaching Mode with Deep Integration of Ideological and Political Education. University Chemistry, 2025, 40(11): 150-159. doi: 10.12461/PKU.DXHX202412113
7. [7]
  Yan Li , Fei Ding , Jing Wang , Jing Nan , Yijun Li , Xiaohang Qiu . Give a Man a Fish, and Teach a Man to Fish: Self-Designed Instrumental Analysis Experiments and Integration of Ideological and Political Elements. University Chemistry, 2024, 39(2): 208-213. doi: 10.3866/PKU.DXHX202310097
8. [8]
  Junyu Peng , Feng Wang , Hongmei Yuan , Xiaoli Sun . Exploration of the “Sheep-Flock Effect” Teaching Model Based on Dual Preview: Taking Instrumental Analysis Experiment Courses in Local Universities as an Example. University Chemistry, 2025, 40(9): 310-317. doi: 10.12461/PKU.DXHX202412098
9. [9]
  Wei Shao , Wanqun Hu , Kaiping Yang , Weiwei Li , Qing Sun , Qiang Zhou , Si Liu , Xiang Sheng , Qingwei Zhang , Pingping Zhu , Wanqun Zhang . Exploration and Reflection on the Course Construction of Comprehensive Instrumental Analysis Experiments for Graduate Students and the Reform of Precision Teaching. University Chemistry, 2026, 41(2): 103-110. doi: 10.12461/PKU.DXHX202503034
10. [10]
  Lihua Jin , Lei Tian , Chaozhan Wang , Jiawei Liu , Quan Bai , Yan Li . Teaching Exploration and Practice of the Instrumental Analysis Experiment “Distinguishing Green Plastic Bags by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy”. University Chemistry, 2026, 41(2): 360-365. doi: 10.12461/PKU.DXHX202502089
11. [11]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
12. [12]
  Kun Li , Na Gao , Shuangyan Huan , Yuzhi Wang . Design of Ideological and Political Education for the Experiment of Detecting Cadmium with Anodic Stripping Voltammetry. University Chemistry, 2024, 39(2): 155-161. doi: 10.3866/PKU.DXHX202307068
13. [13]
  Zhenjun Mao , Haorui Gu , Haiyan Che , Xufeng Lin . Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method. University Chemistry, 2024, 39(4): 81-86. doi: 10.3866/PKU.DXHX202311013
14. [14]
  Lei Shu , Zhengqing Hao , Kai Yan , Hong Wang , Lihua Zhu , Fang Chen , Nan Wang . Development of a Double-Carbon Related Experiment: Preparation, Characterization and Carbon-Capture Ability of Eggshell-Derived CaO. University Chemistry, 2024, 39(4): 149-156. doi: 10.3866/PKU.DXHX202310134
15. [15]
  Haiyang Jin , Yonghai Hui , Yongfei Zhang , Lijun Gao , Yun Wang . Application and Exploration of Nuclear Magnetic Resonance Spectrometer in Undergraduate Basic Laboratory Teaching. University Chemistry, 2025, 40(3): 245-250. doi: 10.12461/PKU.DXHX202406022
16. [16]
  Zhike Yang , Jinfan Xu , Junhao Chen , Zheng Yang , Fei Ding , Neil Qiang Su . AI NMR Assistant: A DP5-Based Intelligent System for NMR Spectral Interpretation. University Chemistry, 2026, 41(1): 20-28. doi: 10.12461/PKU.DXHX202506013
17. [17]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
18. [18]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
19. [19]
  Runjie Li , Hang Liu , Xisheng Wang , Wanqun Zhang , Wanqun Hu , Kaiping Yang , Qiang Zhou , Si Liu , Pingping Zhu , Wei Shao . 氨基酸的衍生及手性气相色谱分离创新实验. University Chemistry, 2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059
20. [20]
  Zhentong Zhu , Peiyao Du , Chaoqin Zeng , Rui Zhou , Xiaoyan He , Bingzhang Lu , Xiaoquan Lu . Discussion on Teaching Methods for Bilingual Courses in Instrumental Analysis for Chemistry Majors. University Chemistry, 2025, 40(10): 39-45. doi: 10.12461/PKU.DXHX202411014

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(1088)
HTML views(86)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return