Citation: Qian Wu, Yuanxia Lv, Zixuan Guo, Zhihao Zhao, Zhimin Zhang, Hongmei Lu. A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses[J]. University Chemistry, ;2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063 shu

A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China

Received Date: 19 November 2024
Revised Date: 20 January 2025

This study presents a research-oriented comprehensive instrumental analysis laboratory course designed within a scientific research context. We developed an integrated experiment combining gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) for metabolomic profiling of plasma small molecules. This approach enables students to master the principles and operational techniques of three analytical instruments (GC, LC, and MS) through a single experiment, effectively addressing the issue of knowledge fragmentation in traditional courses. By incorporating detailed instrument structure explanations and open experimental parameter settings, we significantly enhanced the course’s exploratory nature and actively engaged students' initiative in learning.
- Instrumental analysis laboratory class,
- Research-oriented courses,
- Chromatography coupled with mass spectrometry,
- Metabolomics,
- Blood plasma
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
  Demicheva, E.; Dordiuk, V.; Espino, F. P.; Ushenin, K.; Aboushanab, S.; Shevyrin, V.; Buhler, A.; Mukhlynina, E.; Solovyova, O.; Danilova, I.; et al. Metabolites 2024, 14 (1),
8. [8]
  Roberts, J. L.; Whiley, L.; Gray, N.; Gay, M.; Lawler, N. G. Separations 2022, 9 (7),
9. [9]
  Wu, Q.; Xu, Y.; Ji, H.; Wang, Y.; Zhang, Z.; Lu, H. Anal. Bioanal. Chem. 2019, 411 (23), 6189.
10. [10]
  Ye, S.; Lu, H. Anal. Lett. 2022, 55 (5), 675.
11. [11]
  da Cunha, K. F.; Lanaro, R.; Martins, A. F.; Oliveira, K. D.; Costa, J. L. Forensic Toxicol. 2021, 39 (1), 222.
12. [12]
  Bi, H.; Krausz, K. W.; Manna, S. K.; Li, F.; Johnson, C. H.; Gonzalez, F. J. Anal. Bioanal.l Chem. 2013, 405 (15), 5279.
1. [1]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
2. [2]
  Qijin Mo , Meifang Zhuo , Zhiyi Zhong , Chunfang Gan , Lixia Zhang . Research-Oriented Experimental Teaching in Chemistry Education at Normal University: Taking the Project of Recovering Silver Nitrate from Silver-Containing Waste as an Example. University Chemistry, 2024, 39(6): 201-206. doi: 10.3866/PKU.DXHX202310099
3. [3]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
4. [4]
  Jianan Zhang , Mengzhen Xu , Jiamin Liu , Yufei He . 面向“双碳”目标的脱氯吸附剂开发研究型综合实验设计. University Chemistry, 2025, 40(6): 248-255. doi: 10.12461/PKU.DXHX202408068
5. [5]
  Ronghao Zhao , Yifan Liang , Mengyao Shi , Rongxiu Zhu , Dongju Zhang . Investigation into the Mechanism and Migratory Aptitude of Typical Pinacol Rearrangement Reactions: A Research-Oriented Computational Chemistry Experiment. University Chemistry, 2024, 39(4): 305-313. doi: 10.3866/PKU.DXHX202309101
6. [6]
  Jingyi Chen , Fu Liu , Tiejun Zhu , Kui Cheng . Practice of Integrating Ideological and Political Education into Raman Spectroscopy Analysis Experiment Course. University Chemistry, 2024, 39(2): 140-146. doi: 10.3866/PKU.DXHX202310111
7. [7]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
8. [8]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
9. [9]
  Liqiang Huang , Peng Lin . 数-图分析法解释仪器分析实验课程教学中的难点. University Chemistry, 2025, 40(6): 353-359. doi: 10.12461/PKU.DXHX202407074
10. [10]
  Jingming Li , Bowen Ding , Nan Li , Nurgul . Application of Comparative Teaching Method in Experimental Project Design of Instrumental Analysis Course: A Case Study in Chromatography Experiment Teaching. University Chemistry, 2024, 39(8): 263-269. doi: 10.3866/PKU.DXHX202312078
11. [11]
  Yanan Fan , Jingjing Huang . Interactive Electronic Courseware Facilitates the Development of Integrated Undergraduate-Graduate Instrumental Analysis Laboratory Courses: A Case Study of UV-Vis Spectroscopy Analysis Experiment. University Chemistry, 2025, 40(10): 282-287. doi: 10.12461/PKU.DXHX202411009
12. [12]
  Pingping Zhang , Dong Xiao , Shiyu Zhou , Chuanqiu Tang . Research and Innovative Practice on the Reform of Instrument Analysis Experimental Teaching System for Talent Cultivation in Modern Industry. University Chemistry, 2025, 40(4): 232-238. doi: 10.12461/PKU.DXHX202405179
13. [13]
  Yifeng Xu , Zeying Wu , Guiqin Shang , Linlin Ding , Fuyan Liu , Huan Zhang , Fuhua Jiang . Teaching Reform and Practice of Instrumental Analysis and Experiment Course under the Background of Deep Integration of Industry and Education. University Chemistry, 2025, 40(3): 285-290. doi: 10.12461/PKU.DXHX202408084
14. [14]
  Wanqun Hu , Pingping Zhu , Yuan Zheng , Wanqun Zhang , Wei Shao , Hong Wu , Qiang Zhou , Kaiping Yang , Xiang Sheng . Design and Practice of Ideological and Political Case Study in Instrumental Analysis Experiment Course: the Extraction and Structural Identification of Artemisinin. University Chemistry, 2024, 39(2): 203-207. doi: 10.3866/PKU.DXHX202310062
15. [15]
  Houjin Li , Lin Wu , Xingwen Sun , Yuan Zheng , Zhanxiang Liu , Shuanglian Cai , Ying Xiong , Guangao Yu , Qingwen Liu , Jie Han , Xin Du , Chengshan Yuan , Qihan Zhang , Jianrong Zhang , Shuyong Zhang . Basic Operations and Specification Suggestions for Organic Chemical Chromatography Experiments. University Chemistry, 2025, 40(5): 93-105. doi: 10.12461/PKU.DXHX202408100
16. [16]
  Xiaofei Zhou , Yu-Qing Cao , Feng Zhu , Li Qi , Linhai Liu , Ni Yan , Zhiqiang Zhu . Missions and Challenges of Instrumental Analysis Course in the New Era. University Chemistry, 2024, 39(6): 174-180. doi: 10.3866/PKU.DXHX202310058
17. [17]
  Zhentong Zhu , Peiyao Du , Chaoqin Zeng , Rui Zhou , Xiaoyan He , Bingzhang Lu , Xiaoquan Lu . Discussion on Teaching Methods for Bilingual Courses in Instrumental Analysis for Chemistry Majors. University Chemistry, 2025, 40(10): 39-45. doi: 10.12461/PKU.DXHX202411014
18. [18]
  Yan Su , Xiuyun Wang , Huimin Guo , Yanjuan Zhang , Xinwen Zhang , Yunting Shang , Wenfeng Jiang . To Cultivate Scientific Literacy by Learning, Thinking, Practicing and Understanding, To Utilize the “Smart Eye” Expertise by Integrating of Knowledge and Action: Ideological and Political Construction of Analytical Chemistry Experiment Course. University Chemistry, 2024, 39(2): 196-202. doi: 10.3866/PKU.DXHX202308003
19. [19]
  Zhaoyang Li , Haiyan Zhao , Yali Zhang , Yuan Zhang , Shiqiang Cui . Integration of Nobel Prize Achievements in Analytical Technology with College Instrumental Analysis Course. University Chemistry, 2025, 40(3): 269-276. doi: 10.12461/PKU.DXHX202405131
20. [20]
  Yida Zhang , Haixia Zhang . Exploration and Practice of English Teaching in College Chemistry Courses: Taking the Instrumental Analysis Course as an Example. University Chemistry, 2025, 40(7): 79-82. doi: 10.12461/PKU.DXHX202409090

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(85)
HTML views(11)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142