Citation: Yerong Chen, Bingbin Yang, Xinglei He, Yuqi Lin, Keyin Ye. Enzyme-Directed Evolution Enables Bioconversion of Organosilicon Compounds[J]. University Chemistry, ;2025, 40(10): 121-129. doi: 10.12461/PKU.DXHX202411054 shu

Enzyme-Directed Evolution Enables Bioconversion of Organosilicon Compounds

College of Chemistry, Fuzhou University, Fuzhou 350108, China

Received Date: 19 November 2024
Revised Date: 11 February 2025

Owing to their unique physicochemical properties, organosilicon compounds have found widespread applications in various fields such as electronics, materials science, and pharmaceuticals. However, biological systems typically cannot synthesize or convert most organosilicon compounds under natural conditions, making chemical methods the conventional approach for their synthesis and transformation. In a landmark achievement, Professor Frances H. Arnold, recipient of the 2018 Nobel Prize in Chemistry, and her research team successfully achieved biocatalytic conversion of these non-natural compounds through enzyme-directed evolution. This review systematically examines the team’s research progress in organosilicon compound synthesis and transformation, analyzes the underlying reaction mechanisms, and discusses relevant applications. Our aim is to enhance understanding in this field and facilitate further research in biocatalysis.
- Organosilicon compounds,
- Enzyme,
- Directed evolution,
- Biocatalysis
1. [1]
  Franz, A. K.; Wilson, S. O. J. Med. Chem. 2013, 56 (2), 388.
2. [2]
  Lukevics, E.; Germane, S.; Segal, I.; Zablotskaya, A. Chem. Heterocycl. Compd. 1997, 33 (2), 234.
3. [3]
  Sieburth, S. M.; Nittoli, T.; Mutahi, A. M.; Guo, L. Angew. Chem. Int. Ed. 1998, 37 (6), 812.
4. [4]
  Rücker, C.; Kümmerer, K. Chem. Rev. 2015, 115 (1), 466.
5. [5]
  Lin, Y.; Yu, J.; Ye, K.-Y. Org. Chem. Front. 2024, 11 (24), 7243.
6. [6]
  Lin, Y.; Xue, W.; Li, H.; Su, B.; Lin, J.; Ye, K.-Y. Eur. J. Org. Chem. 2024, 27 (17), e202400003.
7. [7]
  Fessenden, R. J.; Hartman, R. A. J. Med. Chem. 1970, 13 (1), 52.
8. [8]
  Frampton, M. B.; Zelisko, P. M. Silicon 2009, 1 (3), 147.
9. [9]
10. [10]
  Renata, H.; Wang, Z. J.; Arnold, F. H. Angew. Chem. Int. Ed. 2015, 54 (11), 3351.
11. [11]
  Das, A.; Long, Y.; Maar, R. R.; Roberts, J. M.; Arnold, F. H. ACS Catal. 2024, 14 (1), 148.
12. [12]
  Hyster, T. K.; Ward, T. R. Angew. Chem. Int. Ed. 2016, 55 (26), 7344.
13. [13]
  McIntosh, J. A.; Farwell, C. C.; Arnold, F. H. Curr. Opin. Chem. Biol. 2014, 19, 126.
14. [14]
  Wang, Z. J.; Peck, N. E.; Renata, H.; Arnold, F. H. Chem. Sci. 2014, 5 (2), 598.
15. [15]
  Tyagi, V.; Bonn, R. B.; Fasan, R. Chem. Sci. 2015, 6 (4), 2488.
16. [16]
  Kan, S. B. J.; Lewis, R. D.; Chen, K.; Arnold, F. H. Science 2016, 354 (6315), 1048.
17. [17]
  Garcia-Borràs, M.; Kan, S. B. J.; Lewis, R. D.; Tang, A.; Jimenez-Osés, G.; Arnold, F. H.; Houk, K. N. J. Am. Chem. Soc. 2021, 143 (18), 7114.
18. [18]
  Bähr, S.; Brinkmann-Chen, S.; Garcia-Borràs, M.; Roberts, J. M.; Katsoulis, D. E.; Houk, K. N.; Arnold, F. H. Angew. Chem. Int. Ed. 2020, 59 (36), 15507.
19. [19]
  Guo, P.; Cheng, L.-C.; He, X.-L.; Ye, K.-Y. Org. Chem. Front. 2022, 9 (21), 5802.
20. [20]
  Yang, B.-B.; Guo, P.; He, X.-L.; Ye, K.-Y. Org. Chem. Front. 2024, 11 (15), 4125.
21. [21]
  Xu, X.; van Hengst, J. M. A.; Mao, Y.; Martinez, M.; Roda, S.; Floor, M.; Guallar, V.; Paul, C. E.; Alcalde, M.; Hollmann, F. Angew. Chem. Int. Ed. 2023, 62 (24), e202302844.
22. [22]
  Sarai, N. S.; Fulton, T. J.; O’Meara, R. L.; Johnston, K. E.; Brinkmann-Chen, S.; Maar, R. R.; Tecklenburg, R. E.; Roberts, J. M.; Reddel, J. C. T.; Katsoulis, D. E.; et al. Science 2024, 383 (6681), 438.
1. [1]
  Zhaoxin LI , Ruibo WEI , Min ZHANG , Zefeng WANG , Jing ZHENG , Jianbo LIU . Advancements in the construction of inorganic protocells and their cell mimic and bio-catalytical applications. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2286-2302. doi: 10.11862/CJIC.20240235
2. [2]
  Mengyun Wu , Yuxin Deng , Wenhui Wang , Dongdong Xu , Langui Xie , Peipei Sun . 电化学合成4-氯-2-三异丙基硅基喹啉. University Chemistry, 2026, 41(5): 222-229. doi: 10.12461/PKU.DXHX202511044
3. [3]
  Yan Kong , Wei Wei , Lekai Xu , Chen Chen . Electrochemical Synthesis of Organonitrogen Compounds from N-integrated CO₂ Reduction Reaction. Acta Physico-Chimica Sinica, 2024, 40(8): 2307049-0. doi: 10.3866/PKU.WHXB202307049
4. [4]
  Jian Li , Yu Wu , Wenling Gu , Chengzhou Zhu . 纳米酶生物医学应用研究进展. University Chemistry, 2026, 41(9): 173-181. doi: 10.12461/PKU.DXHX202509068
5. [5]
  Jiaming Xu , Yu Xiang , Weisheng Lin , Zhiwei Miao . Research Progress in the Synthesis of Cyclic Organic Compounds Using Bimetallic Relay Catalytic Strategies. University Chemistry, 2024, 39(3): 239-257. doi: 10.3866/PKU.DXHX202309093
6. [6]
  Chonghang Sun , Xingfang Zheng , Lin Li , Xiaolei Jiang , Fangdong Hu . 类比法在有机化学机理教学中的应用——以羰基化合物及羧酸衍生物为例. University Chemistry, 2026, 41(9): 382-391. doi: 10.12461/PKU.DXHX202508095
7. [7]
  Qianlang Wang , Jijun Sun , Qian Chen , Quanqin Zhao , Baojuan Xi . The Appeal of Organophosphorus Compounds: Clearing Their Name. University Chemistry, 2025, 40(4): 299-306. doi: 10.12461/PKU.DXHX202405205
8. [8]
  Mengyang LI , Zhonghao NIU , Hao XU , Jingli XIE . One-pot synthesis of 4H-pyran derivatives catalyzed by viologen-modified metal-organic frameworks. Chinese Journal of Inorganic Chemistry, 2026, 42(7): 1513-1522. doi: 10.11862/CJIC.20250205
9. [9]
  Yingying Wang , Yang Yu . 基于统计能量分析的酶催化机理探究：以丝氨酸水解酶为例. University Chemistry, 2026, 41(9): 373-381. doi: 10.12461/PKU.DXHX202508066
10. [10]
  Feng Han , Fuxian Wan , Ying Li , Congcong Zhang , Yuanhong Zhang , Chengxia Miao . Comprehensive Organic Chemistry Experiment: Phosphotungstic Acid-Catalyzed Direct Conversion of Triphenylmethanol for the Synthesis of Oxime Ethers. University Chemistry, 2025, 40(3): 342-348. doi: 10.12461/PKU.DXHX202405181
11. [11]
  Jiashuang Lu , Xiaoyang Xu , Youqing He , Mingyue Wu , Ruixin Shi , Wenfang Yu , Hang Lu , Ji Liu , Qingzeng Zhu . 生命健康中的有机硅高分子. University Chemistry, 2025, 40(8): 169-180. doi: 10.12461/PKU.DXHX202409143
12. [12]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
13. [13]
  Yongjian Zhang , Fangling Gao , Hong Yan , Keyin Ye . Electrochemical Transformation of Organosulfur Compounds. University Chemistry, 2025, 40(5): 311-317. doi: 10.12461/PKU.DXHX202407035
14. [14]
  Tianqi Sheng , Xuenan Yang , Zhaoyang Li , Shaoguang Zhang . Understanding structural influences in organoelement compounds through configurational inversion of amines, phosphines, and sulfoxides. University Chemistry, 2026, 41(7): 415-429. doi: 10.12461/PKU.DXHX202505012
15. [15]
  Yu Dai , Xueting Sun , Haoyu Wu , Naizhu Li , Guoe Cheng , Xiaojin Zhang , Fan Xia . Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide. University Chemistry, 2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052
16. [16]
  Yijing GU , Huan PANG , Rongmei ZHU . Applications of nickel-based metal-organic framework compounds in supercapacitors. Chinese Journal of Inorganic Chemistry, 2025, 41(10): 2029-2038. doi: 10.11862/CJIC.20250186
17. [17]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
18. [18]
  Chi Li , Jichao Wan , Qiyu Long , Hui Lv , Ying Xiong . N-Heterocyclic Carbene (NHC)-Catalyzed Amidation of Aldehydes with Nitroso Compounds. University Chemistry, 2024, 39(5): 388-395. doi: 10.3866/PKU.DXHX202312016
19. [19]
  Xiaomei Ai , Muran Lin , Jinlan Zeng , Jiwei Ren . 可见光催化绿色合成二肽化合物. University Chemistry, 2026, 41(5): 358-367. doi: 10.12461/PKU.DXHX202510007
20. [20]
  Yihui Song , Shangshang Qin , Kai Wu , Chengyun Jin , Bin Yu . 生物化学在高水平创新型药学人才培养中的交叉融合应用——以去甲基化酶LSD1抑制剂的活性评价为例. University Chemistry, 2025, 40(6): 341-352. doi: 10.12461/PKU.DXHX202406018

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(1452)
HTML views(115)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142