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Citation: Runjie Li, Hang Liu, Xisheng Wang, Wanqun Zhang, Wanqun Hu, Kaiping Yang, Qiang Zhou, Si Liu, Pingping Zhu, Wei Shao. 氨基酸的衍生及手性气相色谱分离创新实验[J]. University Chemistry, ;2025, 40(6): 286-295. doi: 10.12461/PKU.DXHX202407059 shu

氨基酸的衍生及手性气相色谱分离创新实验

Runjie Li ² ,
Hang Liu ² ,
Xisheng Wang ² ,
Wanqun Zhang ¹ ,
Wanqun Hu ¹ ,
Kaiping Yang ¹ ,
Qiang Zhou ¹ ,
Si Liu ¹ ,
Pingping Zhu ² ,
Wei Shao ¹

1.
National Demonstration Center for Experimental Chemistry Education (University of Science and Technology of China), Hefei 230026, China;
2.
School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China

Received Date: 11 July 2024
Revised Date: 9 December 2024

氨基酸是蛋白质的重要组成单元。氨基酸的手性分子对映体往往有着类似的物理性质，但却具有完全不同的药理作用，因此，氨基酸的手性分离对于医药及生命科学有着极其重要的意义。手性气相色谱是氨基酸手性分离的重要手段。本实验使用氯甲酸乙酯(ECF)作为衍生化试剂，将氨基酸衍生化方法及手性分离引入到研究生“综合仪器分析实验”教学之中，可以让学生了解气相色谱从样品前处理、定性分析、定量检测到实验数据处理整个分析过程，在掌握基本操作的基础上进行探究，提升研究生的科研操作技能。
- Amino acids,
- Chiral gas chromatography,
- Ethyl chloroformate,
- Derivatization
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