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Citation: Chenghe Yang, Yi Lü, Rui Liu. The Rise to Fame of Digital PCR[J]. University Chemistry, ;2025, 40(4): 340-345. doi: 10.12461/PKU.DXHX202406111 shu

The Rise to Fame of Digital PCR

Chenghe Yang ¹ ,
Yi Lü ^1,2 ,
Rui Liu ¹

1.
College of Chemistry, Sichuan University, Chengdu 610064, China;
2.
Analytical & Testing Center, Sichuan University, Chengdu 610064, China

Received Date: 27 June 2024
Revised Date: 27 June 2024

Digital PCR (Polymerase Chain Reaction) enables highly sensitive nucleic acid detection, allowing for the analysis of individual molecules. With its unique capability for absolute quantitation and high sensitivity, digital PCR has emerged as a powerful tool in biomolecular research. This article presents digital PCR from a first-person perspective, allowing readers to appreciate the evolution, technical principles, and applications of digital PCR during the pandemic.
- Digital PCR,
- Single-molecule detection,
- Low-abundance detection,
- High sensitivity,
- Absolute quantitation
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