Citation: LIU Zhirong. Evaluation on Efficiencies of 11 Purification Methods Toward 50 Pesticide Residues in Angelica sinensis by Ultra-high Performance Liquid Chromatography/Tandem Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, ;2019, 36(8): 968-976. doi: 10.11944/j.issn.1000-0518.2019.08.190011 shu

Evaluation on Efficiencies of 11 Purification Methods Toward 50 Pesticide Residues in Angelica sinensis by Ultra-high Performance Liquid Chromatography/Tandem Mass Spectrometry

LIU Zhirong ^,

Gansu Provincial Institute of Drug Control, Lanzhou 730070, China

Corresponding author: LIU Zhirong, 553111058@qq.com
Received Date: 14 January 2019
Revised Date: 25 March 2019
Accepted Date: 23 April 2019

Figures(4)

Fifty pesticides in Angelica sinensis, were determined by ultra-high performance liquid chromatography/tandem mass spectrometry(UPLC-MS/MS). Through comparing 11 kinds of purification methods, the best effective matrix purification method was selected. The pesticide residues were extracted from samples with acetoniteile, cleaned-up with NaCl and then analyzed using UPLC-MS/MS in dynamic multiple reaction monitoring(dMRM) mode with positive and negative electrospray ionization. The pesticide residues were quantified by matrix matched standard solution-internal standard method. All calibration curves showed good linearity(R² > 0.99) within the test ranges. The average recoveries of most pesticides at the spiked levels of 10, 50, 100 μg/kg ranged from 70.1%~117.7% with RSDs of 1.0%~20.0%. The limits of quantification of 50 pesticides were 1.0~20.0 μg/kg. The method provides a reliable basis for accurate, efficient and economical detection of targeted analytes in Angelica sinensis.
- matrix purification method,
- pesticide residues,
- Angelica sinensis
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