Citation: ZHAO Mohan, LIU Zhiqiang, GENG Aifang, XIE Wenbing. Identification and Content Determination of Active Constituents of Fig Leaves[J]. Chinese Journal of Applied Chemistry, ;2018, 35(11): 1391-1398. doi: 10.11944/j.issn.1000-0518.2018.11.180051 shu

Identification and Content Determination of Active Constituents of Fig Leaves

ZHAO Mohan ^a,b ,
LIU Zhiqiang ^b ,
GENG Aifang ^a,, ,
XIE Wenbing ^b,,

a.
School of Chemical and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, China
b.
National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of AppLied, Chemistry Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: GENG Aifang, gengaf687@Sina.com XIE Wenbing, xiewenbing@ciac.ac.cn
Received Date: 26 February 2018
Revised Date: 19 April 2018
Accepted Date: 5 June 2018

Fund Project: the National Natural Science Foundation of China 81473537Supported by the National Natural Science Foundation of China(No.81473537)

Figures(3)

In recent years, studies on the extraction and analysis of the components contained in fig leaves have focused on the analysis of the same type or the whole group of single compounds, and there are few qualitative and quantitative studies on single effective substances in various types. In this study, solvent extraction-ultrasonic-assisted extraction was used to extract the active ingredients of fig leaves, and the active ingredients were identified by gas chromatography-mass spectrometry and ultrahigh performance liquid chromatography-ion trap-mass spectrometry. The results show that the main components of fig leaves are chlorogenic acid, rutin, psoralen and bergapten. The single factor experiments were used to determine the best extraction process for the four components. A quantitative method for the determination of chlorogenic acid, rutin, psoralen and bergapten was established by high performance liquid chromatography. The linear relationship is obtained for chlorogenic acid in the range of 1.84~9.2 μg (R²=0.9991) with recovery rate of 95.2%, rutin in the range of 1.84~9.2 μg (R²=0.9981) with a recovery rate of 96.8%, bergapten in the range of 4~20 μg (R²=0.9998) with a recovery rate of 89.5%, and psoralen in the range of 4~20 μg (R²=0.9963) with a recovery rate of 87.1%, respectively.
- fig leaves,
- chlorogenic acid,
- rutin,
- psoralen,
- bergapten
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