Citation: YE Lifang, WU Quanzhou. Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls[J]. Chinese Journal of Applied Chemistry, ;2018, 35(11): 1309-1316. doi: 10.11944/j.issn.1000-0518.2018.11.170371 shu

Modification of Ordered Macroporous Silica by a Functional Polymer Layer and Immobilization of Glucoamylase on the Macropore Walls

YE Lifang ,
WU Quanzhou ^,

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

Corresponding author: WU Quanzhou, gzywqz@gzucm.edu.cn
Received Date: 18 October 2017
Revised Date: 8 December 2017
Accepted Date: 22 January 2018

Fund Project: the Guangdong Province Natural Science Foundation of China 2017A030313675the Youth Elite Project of GUCM AFD015151Z1450Supported by the National Natural Science Foundation of China(No.81303199), the Guangdong Province Natural Science Foundation of China(No.2017A030313675), the Youth Elite Project of GUCM(No.AFD015151Z1450)the National Natural Science Foundation of China 81303199

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This paper presented a facile method to the modification of the three-dimensionally ordered macroporous (3DOM) SiO₂ hybrids with a carboxyl functionalized polymer layer on the macropore walls. Acrylic acid and glycerol 1, 3-diglycerolate diacrylate were copolymerized to form the polymer layer in the macropores. Raman spectra, scanning electron microscopy (SEM) and BET measurements show that the prepared 3DOM SiO₂@polymer composites (SiO₂-COOH) have an uniform interconnected macroporous structure and the macropore walls are covered by a nanoscaled compact polymer layer. Moreover, the 3DOM SiO₂-COOH has an improved mechanical strength. The 3DOM SiO₂-COOH was further used as the support to immobilize glucoamylase. The results show that the immobilized enzyme homogeneously distributes in the 3DOM materials. The optimum pH of immobilized and free enzymes is both at 5, and the optimal reaction temperature is at 55℃. Michaelis constants of the immobilized enzyme and free enzyme are 3.78 g/L and 3.97 g/L, respectively. The immobilized enzyme presents higher thermal, pH, storage stabilities and higher reusability compared with the free enzyme. The results indicate that 3DOM SiO₂-COOH could be a novel support for the immobilization of enzymes.
- ordered macropore,
- surface modification,
- composite,
- immobilized enzyme
1. [1]
  Ozdural A R, Tanyolac D, Boyaci I H. Determination of Apparent Kinetic Parameters for Competitive Product Inhibition in Packed-Bed Immobilized Enzyme Reactors[J]. Biochem Eng J, 2003,14(1):27-36. doi: 10.1016/S1369-703X(02)00099-2
2. [2]
  Yamada R, Suzuki Y, Yasuda M. Immobilization of Proteins on Synthetic Resins Using Super-critical Carbon Dioxide[J]. J Supercrit Fluids, 2016,107:566-570. doi: 10.1016/j.supflu.2015.07.016
3. [3]
  Huttner S, Gomes M Z D V, Iancu L. Immobilisation on Mesoporous Silica and Solvent Rinsing Improve the Transesterification Abilities of Feruloyl Esterases from Myceliophthora Thermophila[J]. Bioresour Technol, 2017,239:57-65. doi: 10.1016/j.biortech.2017.04.106
4. [4]
  Vasconcellos A D, Paula A S, Filho R A L. Synergistic Effect in the Catalytic Activity of Lipase Rhizomucor Miehei Immobilized on Zeolites for the Production of Biodiesel[J]. Micropor Mesopor Mater, 2012,163:343-355. doi: 10.1016/j.micromeso.2012.07.043
5. [5]
  Shahrestani H, Taheri-kafrani A, Soozanipour A. Enzymatic Clarification of Fruit Juices Using Xylanase Immobilized on 1, 3, 5-Triazine-Functionalized Silica-Encapsulated Magnetic Nanoparticles[J]. Biochem Eng J, 2016,109:51-58. doi: 10.1016/j.bej.2015.12.013
6. [6]
  Velev O D, Jede T A, Lobo R F. Porous Silica via Colloidal Crystallization[J]. Nature, 1997,389(6650):447-448.
7. [7]
  Pandya P H, Jasra R V, Newalkar B L. Studies on the Activity and Stability of Immobilized Alpha-Amylase in Ordered Mesoporous Silicas[J]. Micropor Mesopor Mater, 2005,77(1):67-77. doi: 10.1016/j.micromeso.2004.08.018
8. [8]
  Yan W, Zheng X H, Min Z. Study of Immobilization of Laccase on Mesoporous Molecular Sieve MCM-41[J]. J Chem Eng Chinese Univ, 2008,22(1):83-87.
9. [9]
  Schroden R C, Al-daous M, Blanford C F. Optical Properties of Inverse Opal Photonic Crystals[J]. Chem Mater, 2002,14(8):3305-3315. doi: 10.1021/cm020100z
10. [10]
  Yang J, Chen Z L, Wang H. Highly Ordered Three-Dimensional TiO₂@C Nanotube Arrays as Freestanding Electrode for Sodium-Ion Battery[J]. Mater Lett, 2017,207:149-152. doi: 10.1016/j.matlet.2017.07.066
11. [11]
  Li X, Liu Y, Deng J. Enhanced Catalytic Performance for Methane Combustion of 3DOM CoFe₂O₄ by Co-Loading MnO_x and Pd-Pt Alloy Nanoparticles[J]. App Surf Sci, 2017,403:590-600. doi: 10.1016/j.apsusc.2017.01.237
12. [12]
  Ye P, Wan R B, Wang X P. Quantitative Enzyme Immobilization:Control of the Carboxyl Group Density on Support Surface[J]. J Mol Catal B:Enzym, 2009,61(3/4):296-302.
13. [13]
  Gornowich D B, Blanchard G J. Enhancement of Enzyme Activity by Confinement in an Inverse Opal Structure[J]. J Phys Chem C, 2012,116(22):12165-12171. doi: 10.1021/jp303271n
14. [14]
  Jiang Y, Wang Y, Wang H. Facile Immobilization of Enzyme on Three Dimensionally Ordered Macroporous Silica via a Biomimetic Coating[J]. New J Chem, 2015,39(2):978-984. doi: 10.1039/C4NJ01947D
15. [15]
  Wu Q, Liao J, Yin Q. Synthesis, Characterization and Catalytic Activity of Ordered Macroporous Silicas Functionalized with Organosulfur Groups[J]. Mater Res Bull, 2008,43(5):1209-1217. doi: 10.1016/j.materresbull.2007.05.025
16. [16]
  Lowe A B. Thiol-Yne Click'/Coupling Chemistry and Recent Applications in Polymer and Materials Synthesis and Modification[J]. Polymer, 2014,55(22):5517-5549. doi: 10.1016/j.polymer.2014.08.015
17. [17]
  Heggli M, Tirelli N, Zisch A. Michael-Type Addition as a Tool for Surface functionalization[J]. Bioconjug Chem, 2003,14(5):967-973. doi: 10.1021/bc0340621
18. [18]
  Berkel K Y, Hawker C J. Tailored Composite Polymer-Metal Nanoparticles by Miniemulsion Polymerization and Thiol-ene Functionalization[J]. J Polym Sci Polym Chem, 2010,48(7):1594-1606. doi: 10.1002/pola.v48:7
19. [19]
  Reddy P S, Rani D J, Sulthana S. Amylases as a Tool for Industrial Application:A Review[J]. J Pure Appl Algebra, 2011,5(1):167-171.
20. [20]
  Amirbandeh M, Taheri-kafrani A. Immobilization of Glucoamylase on Triazine-Functionalized Fe₃O₄/Graphene Oxide Nanocomposite:Improved Stability and Reusability[J]. Int J Biol Macromol, 2016,93:1183-1191. doi: 10.1016/j.ijbiomac.2016.09.092
21. [21]
  Isobe N, Lee D S, Kwon Y J. Immobilization of Protein on Cellulose Hydrogel[J]. Cellulose, 2011,18(5):1251-1256. doi: 10.1007/s10570-011-9561-8
22. [22]
  YE Shuaiguan, SUN Zuoyu, DING Yi. The Coupled Reaction of Glucose Oxidase-Per-Oxidase-o-Diansidine System for Determination of Blood-Glucose and Some Inhibitors[J]. Chem Reagent, 1986,8(5):306-309.
23. [23]
  Nair D P, Podgorski M, Chatani S. The Thiol-Michael Addition Click Reaction:A Powerful and Widely Used Tool in Materials Chemistry[J]. Chem Mater, 2014,26(1):724-744. doi: 10.1021/cm402180t
24. [24]
  Gomez J L, Bodalo A, Gomez E. Immobilization of Peroxidases on Glass Beads:An Improved Alternative for Phenol Removal[J]. Enzyme Microb Technol, 2006,39(5):1016-1022. doi: 10.1016/j.enzmictec.2006.02.008
25. [25]
  GUO Ming, YAN Bingyu, WANG Chunpeng. Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance[J]. Sci Silvae Sin, 2013,49(11):122-128.
26. [26]
  Volokitina M V, Korzhikov-Vlakh V A, Tennikova T B. Macroporous Monoliths for Biodegradation Study of Polymer Particles Considered as Drug Delivery Systems[J]. J Pharm Biomed Anal, 2017,145:169-177. doi: 10.1016/j.jpba.2017.06.031
27. [27]
  Gashtasbi F, Ahmadian G, Noghabi K A. New Insights into the Effectiveness of Alpha-Amylase Enzyme Presentation on the Bacillus Subtilis Spore Surface by Adsorption and Covalent Immobilization[J]. Enzyme Microb Technol, 2014,64/65:17-23. doi: 10.1016/j.enzmictec.2014.05.006
28. [28]
  Gupta K, Jana A K, Kumar S. Solid State Fermentation with Recovery of Amyloglucosidase from Extract by Direct Immobilization in Cross Linked Enzyme Aggregate for Starch Hydrolysis[J]. Biocatal Agric Biotechnol, 2015,4(4):486-492.
29. [29]
  WANG Feng, LIANG Liman, GU Zhengbiao. Immobilization of Amylase on PS-TEPA and PS-PEG Resins[J]. Chinese J Appl Chem, 2008,25(11):1259-1265. doi: 10.3969/j.issn.1000-0518.2008.11.003
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