Citation: CAI Qihong, CHEN Xiaoping, LU Jiaxing, CHEN Dengxiu. Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry[J]. Chinese Journal of Applied Chemistry, ;2018, 35(5): 609-612. doi: 10.11944/j.issn.1000-0518.2018.05.170210 shu

Rapid Determination of Total Amount of Three Estrogens in Milk Powder by Fluorescence Spectrometry

Key Laboratory of Pharmaceutical Analysis and Laboratory Medicine of Fujian Province University, Pharmaceutical and Medical Technology College, Putian University, Putian, Fujian 351100, China

Corresponding author: CAI Qihong, cqh118@163.com
Received Date: 15 June 2017
Revised Date: 7 August 2017
Accepted Date: 4 September 2017

Fund Project: Supported by the Leading Project of Fujian Provincial Science and Technology Program(No.2015Y0030), the Scientific Research Project of the Education Departerment of Fujian Province(No.JK2014045), the Science and Technology Project of Putian City[No.2013S01(2)], the Scientific Research Project of Putian University(No.2015088)the Scientific Research Project of the Education Departerment of Fujian Province JK2014045the Science and Technology Project of Putian City 2013S01(2)the Scientific Research Project of Putian University 2015088the Leading Project of Fujian Provincial Science and Technology Program 2015Y0030

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Based on the similarity of the fluorescence properties of three estrogens, a new fluorescence method for direct determination of the total amount of three estrogens(estradiol, estriol, ethinyloestradiol) in milk powder was developed. Taking estriol as the standard reference material, the standard curve method was adopted. The fluorescence intensity of estriol has a linear relationship with the concentration in the range of 1.00~2000 nmol/L and the detection limit of 0.16 nmol/L. The recovery is in the range of 89.11%~98.49%. This method is simple, rapid, highly accurate and sensitive and has been successfully applied to determine the total estrogens in milk powder with satisfactory results.
- estrogens,
- fluorescence spectrometry,
- milk powder,
- estradiol,
- estriol,
- ethinyloestradiol
1. [1]
  Armstrong B, Doll R. Environmental Factors and Cancer Incidenceandmortality in Different Countries, with Special Reference to Dietary Practices[J]. Int J Cancer, 1975,15(4):617-631. doi: 10.1002/(ISSN)1097-0215
2. [2]
  Rose D P, Boyar A P, Wynder E L. International Comparisons of Mortality Rates for Cancer of the Breast, Ovary, Prostate, and Colon, and Per Capita Food Consumption[J]. Cancer, 1986,58(11):2363-2371. doi: 10.1002/(ISSN)1097-0142
3. [3]
  Qin L Q, Wang P Y, Kaneko T. Estrogen:One of the Risk Factors in Milk for Prostate Cancer[J]. Med Hypoth, 2004,62(1):133-142. doi: 10.1016/S0306-9877(03)00295-0
4. [4]
  YUAN Lijun, XU Zhuangjian, XU Xiangsheng. The Estrogen in Cow's Milk and Its Safety[J]. Int J Inter Med, 2007,34(4):712-714.
5. [5]
  Kaipparettu B A, Malik S, Konduri S D. Estrogenmediated Downregulation of CD24 in Breast Cancer Cells[J]. Int J Cancer, 2008,123(1):66-72. doi: 10.1002/(ISSN)1097-0215
6. [6]
  Wang L, Yan H Y, Yang C L. Synthesis of Mimic Molecularly Imprinted Ordered Mesoporous Silica Adsorbent by Thermally Reversible Semicovalent Approach for Pipette-Tip Solid-Phase Extraction-Liquid Chromatography Fluorescence Determination of Estradiol in Milk[J]. J Chromatogr A, 2016,1456(1):58-67.
7. [7]
  Xiao X H, Yin Y, Hu Y L. Microwave-Assisted Extraction Coupled with Single Drop Microextraction and High-Performance Column Liquid Chromatography for the Determination of Trace Estrogen Adulterants in Soybean Isoflavone Dietary Supplements[J]. J AOAC Int, 2010,93(3):849-854.
8. [8]
  XIAO Fugang, GUO Weiyun, LIU Yan. Determination of Estradiol in Milk Powder by HPLC[J]. Food Res Dev, 2011,32(8):81-83.
9. [9]
  ZHANG Hongying, ZHANG Hong, WANG Shuhui. Determination of Environmental Estrogens in Milk by Gas Chromatography-Mass Spectrometry[J]. Chinese J Health Lab Technol, 2009,19(8):1713-1715.
10. [10]
  LIU Yonglin, TIAN Chengxin, YAN Tianwen. Determination of β-Estradiol in Bovine Colostrum by Gas Chromatography-Mass Spectrometry[J]. China Dairy Ind, 2014,42(7)53/54/55, 59.
11. [11]
  Tso J, Aga D S. A Systematic Investigation to Optimize Simultaneous Extraction and liquid Chromatography Tandem Mass Spectrometry Analysis of Estrogens and Their Conjugated Metabolites in Milk[J]. J Chromatogr A, 2010,1217(29):4784-4795. doi: 10.1016/j.chroma.2010.05.024
12. [12]
  Yang Y, Shao B, Zhang J. Determination of the Residues of 50 Anabolic Hormones in Muscle, Milk and Liver by Very-High-Pressure Liquid Chromatodraphy-Electrospray Ionization Tandem Mass Spectrometry[J]. J Chromatogr B, 2009,877(5/6):489-496.
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沈阳化工大学材料科学与工程学院沈阳 110142