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Citation: MEI Xiao-Hong, TIAN Jing-Jing, ZHANG Yang-Zi, DU Zai-Hui, ZHANG-Yuan, XU Wen-Tao. Ultrasensitive and Colorimetric Detection of Copper(Ⅱ) Based on Dual-DNAzymes[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(12): 1945-1952. doi: 10.11895/j.issn.0253-3820.181582 shu

Ultrasensitive and Colorimetric Detection of Copper(Ⅱ) Based on Dual-DNAzymes

1.
College of Food Science and Nutritional Engineering, Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China;
2.
Key Laboratory of Safety Evaluation(Food) of Agricultural Genetically Modified Organisms, Ministry of Agriculture, China Agricultural University, Beijing 100083, China

Received Date: 7 September 2018
Revised Date: 29 October 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No.31671922).

Copper is an essential element for life and constitutes copper proteins, and attends life activities in human body. However, an excess of copper is poisonous, and can cause hepatitis, gastroenteritis and pneumonia. copper enters into biosphere from ores by weathering and erosion and exists in water in the form of a divalent copper ion. Therefore, it is necessary to detect copper ions in an ultrasensitive and quantitative way. Herein, a dual-functional DNAzymatic visual biosensor was developed, optimized and validated to detect divalent copper ions sensitively and selectively in tap water. In the method, dual DNAzymes with different functions were designed within one biosensor. One DNAzyme was Cu²⁺-dependent cleaving DNAzyme, which could be cleaved by Cu²⁺ for signal recognition. The other one was G-quadruplex DNAzyme, which was induced by terminal deoxynucleotidyl transferase (TdT) for "one-to-multiplex" signal amplification and output. In the middle of two steps, the exponential amplification reaction (EXPAR) was applied to amplify the recognitive signal exponentially. Under optimized conditions, Cu²⁺ could be selectively observed with the naked eyes with the detection limit (LOD) of 10 pmol/L and with the microplate reader with LOD of 7.6 pmol/L in about 2 h. Furthermore, Cu²⁺ in the concentration range of 10 pmol/L-200 pmol/L in tap water samples could be discriminated by naked eyes. The platform provides an ultrasensitive and colorimetric detective tool for the analysis of trace Cu²⁺in tap water with high specificity, and will have broad application prospect in many fields.
- Copper ion,
- DNAzyme,
- Terminal deoxynucleotidyl transferase,
- Isothermal amplification
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