Citation: LI Zhu-Heng, GAO Jing-Qing, JIAN Ming-Hong, WANG Zhen-Xin. Fabrication of Lectin Microarray for Studying Interactions of Antibiotics with Staphylococcus aureus by Gold Nanoparticle Probes[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(12): 1904-1912. doi: 10.11895/j.issn.0253-3820.181570 shu

Fabrication of Lectin Microarray for Studying Interactions of Antibiotics with Staphylococcus aureus by Gold Nanoparticle Probes

1.
Jilin Provincial Institute of Education, Changchun 130024, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received Date: 3 September 2018
Revised Date: 12 October 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21475126).

The lectin microarray-based resonance light scattering (RLS) assay has been developed to evaluate the interactions of Staphylococcus aureus (S. aureus) with three broad-spectrum antibiotics (amoxicillin, vancomycin and streptomycin). S. aureus was firstly captured by immobilized lectins on the substrate surface, and then labeled with Griffonia simplicifolia Ⅱ (GSⅡ) modified 38.5 nm gold nanoparticles (GNP@GSⅡ) for generating RLS signal. The investigation of the binding affinity of S. aureus with 16 lectins in the presence of antibiotics showed that amoxicillin and vancomycin could significantly reduce the expression level of glycans on S. aureus membrane, while streptomycin could increase the expression level of glycans on S. aureus membrane. The experimental result may suggest the different action mechanisms of antibiotics with S. aureus. Furthermore, the minimum inhibitory concentration (MIC) values and bactericidal efficiency of antibiotics could also be determined by the drug induced change of RLS intensity of GSⅡ subarray and lectin binding patterns of S. aureus, respectively.
- Lectin microarrays,
- Staphylococcus aureus,
- Antibiotic,
- Gold nanoparticles,
- Resonance light scattering
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沈阳化工大学材料科学与工程学院沈阳 110142