高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用

引用本文: 张君才, 陈佑宁, 卫引茂. 高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用[J]. 分析化学, 2018, 46(9): 1464-1471. doi: 10.11895/j.issn.0253-3820.181369 shu

Citation: ZHANG Jun-Cai, CHENG You-Ning, WEI Yin-Mao. Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1464-1471. doi: 10.11895/j.issn.0253-3820.181369 shu

高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用

1.
咸阳师范学院化学与化工学院, 咸阳 712000;
2.
陕西省现代分离科学重点实验室, 西北大学化学与材料科学学院, 西安 710127
基金项目:
本文系国家自然科学基金项目（Nos.21475104，21775121）和陕西省现代分离科学重点实验室基金项目（No.17JS134）资助

摘要: 硼亲和吸附法是分离富集邻羟基物质的重要方法。为提高吸附剂的吸附容量，本研究采用聚乙烯亚胺修饰磁性纳米粒子，增加粒子表面引发剂的密度，再通过表面引发原子转移自由基聚合法（SI-ATRP）将3-丙烯酰基苯硼酸原位聚合在纳米粒子表面，制备了一种高密度聚合物分子刷型的硼酸亲和磁性纳米粒子吸附剂。采用磁性分散固相萃取法对非邻羟基和邻羟基物质的混合液进行富集，发现此吸附剂对邻羟基小分子和生物大分子具有良好的吸附特异性；采用吸附等温线法测定了吸附剂的吸附容量，发现此吸附剂对邻苯二酚、腺苷和卵清蛋白的吸附容量分别为（151±32）μmol/g、（123±18）μmol/g和1.5 μmol/g，远高于传统吸附剂的吸附容量。采用所制备的硼酸亲和磁性纳米粒子对尿液中4种核苷和蛋清中的糖蛋白进行萃取，结果表明，此吸附剂能够有效去除生物样品中的干扰物，且对核苷具有较高的萃取回收率（83.8%-108.7%，RSD < 15%），对糖蛋白有特异性富集作用，说明此吸附剂在生物样品的选择性富集中具有良好应用潜能。

关键词:

English

Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules

1.
College of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang 712000, China;
2.
Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China
Received Date: 02 June 2018
Revised Date: 19 July 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 21475104, 21775121) and the Key Laboratory of Modern Separation Science in Shaanxi Province, China (No.17JS134).

Abstract: Boronate affinity is an important method to extract selectively cis-diol biomolecules. In this work, a novel polymeric brush-type magnetic adsorbent with high density of boronate was successfully prepared. Specifically, polyethyleneimine was firstly employed to supply a large quantity of initiator sites on the surface of the magnetic nanoparticales, and then 3-acrylamidophenylboronic acid (AAPBA) monomer was grafted from the surface of magnetic nanoparticales using surface-initiated atom transfer radical polymerization (SI-ATRP). The mixture of cis-diol and non-cis-diol compounds was extracted by magnetic dispersion solid-phase extraction method, and it was found that the obtained adsorbent showed good selectivity to cis-diol small molecules and biomacromolecules. In addition, the binding capacity was measured by the adsorption isotherm method to be (151±32) mmol/g for catechol, (123±18) mmol/g for adenosine and 1.5 μmol/g for ovalbumin, respectively, which was far higher than those reported in the references. Finally the prepared nanoparticles were employed to extract four nucleosides in urine and glycoproteins in egg white, respectively. The results showed that the nanoparticles could effectively remove some potential interference in biological samples. In addition, the developed method exhibited good recovery for four nucleosides (83.8%-108.7%, RSD<15%) and excellent selectivity for glycoprotein. The adsorbents had potential in selective enrichment of cis-diol compounds from real biological samples.

Key words:

1. [1]
  ZHANG Ying, LIU Ying, LIU Yang, QIANG Shan, YANG Bing-Ying, HUANG Lin-Juan, WANG Zhong-Fu. Chinese J. Anal. Chem.,2016,44(2):265-272 张英, 刘影, 刘洋, 强珊, 杨冰莹, 黄琳娟, 王仲孚.分析化学,2016,44(2):265-272
2. [2]
  Mann B F, Mann A K P, Skrabalak S E, Novotny M V. Anal. Chem.,2013,85(3):1905-1912
3. [3]
  Wang Y L, Liu M B, Xie L Q, Fang C Y, Xiong H M, Lu H J. Anal. Chem.,2014,86(4):2057-2064
4. [4]
  LI Tian-Tian, ZHANG Li-Yuan, YU Zhen-Long, MA Xiao-Chi, DENG Sa. Chinese J. Anal. Chem.,2017,45(9):1259-1263 李甜甜, 张丽媛, 于振龙, 马骁驰, 邓卅.分析化学,2017,45(9):1259-1263
5. [5]
  Zhang L J, Jiang H C, Yao J, Wang Y L, Fang C Y, Yang P Y, Lu H J. Chem. Commun.,2014,50(8):1027-1029
6. [6]
  Xiong Z C, Zhao L, Wang F J, Zhu J, Qin H Q, Wu R A, Zhang W B, Zou H F. Chem. Commun.,2012,48(65):8138-8140
7. [7]
  Wells L,Vosseller K, Cole R N, Cronshaw J M, Matunis M J, Hart G W. Mol. Cell. Proteomics,2002,1(10):791-804
8. [8]
  Lin Z A, Zheng J N, Lin F, Zhang L, Cai Z W, Chen G N. J. Mater. Chem.,2011,21(2):518-524
9. [9]
  Zhang S T, He X W, Chen L X, Zhang Y K. New J. Chem.,2014,38(9):4212-4218
10. [10]
  DUAN Yu-Hui, WEI Yin-Mao. Chinese J. Anal. Chem.,2013,41(3):406-411 段语晖卫引茂.分析化学,2013,41(3):406-411
11. [11]
  Zhu H J, Hao H, Xia K X, Liu J X, Yin X L, Zhang W L, Pan J M. Chem. Eng. J., 2018,346(28):317-328
12. [12]
  WEI Yin-Mao, BO Chun-Miao, WANG Chao-Zhan, Journal of Northwest University(Natural Science Edition),2017,47(6):838-851 卫引茂, 卜春苗, 王超展.西北大学学报(自然科学版), 2017,47(6):838-851
13. [13]
  Wang W, He M F, Wang C Z, Wei Y M. Anal. Chim. Acta,2015,886:66-74
14. [14]
  Dou P, Liang L, He J G, Liu Z, Chen H Y. J. Chromatogr. A,2009,1216(44):7558-7563
15. [15]
  Wang C Z, Xu H H, Wei Y M. Anal. Chim. Acta, 2016,902:115-122
16. [16]
  CHEN Lin-Ji, ZHU Chao-Le, CAO Xiao-Ji, ZHOU Ting, YE Xue-Min, MO Wei-Min. Chinese J. Anal.Chem.,2017,45(5):762-769 陈林吉, 朱晁乐, 曹小吉, 周婷, 叶学敏, 莫卫民.分析化学,2017,45(5):762-769
17. [17]
  Zoppe J O, Ataman N C, Mocny P, Wang J, Moraes J, Klok H A. Chem. Rev.,2017,117(3):1105-1318
18. [18]
  Xue S, Wang C Z, Wei Y M. RSC Adv.,2017,7(20):11921-11928
19. [19]
  Georgiou C D, Grintzalis K, Zervoudakis G. Papapostolou I. Anal. Bioanal. Chem.,2008,391(1):391-403
20. [20]
  Du J, He M F, Wang X M, Fan H, Wei Y M. Biomed. Chromatogr.,2015,29(2):312-320
21. [21]
  He H B, Sun Y R, Li B, Yu Q W, Wang T L, Feng Y Q. Anal. Methods,2013,5(6):1435-1441
22. [22]
  Cetinkaya O, Duru M E, Cicek H. J. Chromatogr. B,2012,909:51-60
23. [23]
  Li Q J, Lu C C, Li H Y, Liu Y C, Wang H Y, Wang X, Liu Z. J. Chromatogr. A,2012,1256:114-120
24. [24]
  Yang F, Lin Z, He X W, Chen L X, Zhang Y K. J. Chromatogr. A,2011,1218:9194-9201
25. [25]
  Li H, Shan Y H, Qiao L Z, Dou A, Shi X Z, Xu G W. Anal. Chem.,2013,85(23):11585-11592
26. [26]
  Wang H Y, Bie Z J, Lu C C, Liu Z. Chem. Sci.,2013,4(11):4298-4303
27. [27]
  Cheng T, Zhu S Q, Zhu B, Liu X Y, Zhang H X. J. Sep. Sci.,2016,39(7):1347-1356
28. [28]
  Liebich H M, Di Stefano C, Wixforth A, Schmid H R. J. Chromatogr. A,1997,763(1-2):193-197
29. [29]
  Gonzalez O, Blanco M E, Iriarte G, Bartolomé L, Maguregui M I, Alonso R M. J. Chromatogr. A,2014,1353:10-27

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用

English

Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用

English

Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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