Citation: ZHANG Jun-Cai, CHENG You-Ning, WEI Yin-Mao. Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(9): 1464-1471. doi: 10.11895/j.issn.0253-3820.181369 shu

Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules

1.
College of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang 712000, China;
2.
Key Laboratory of Modern Separation Science in Shaanxi Province, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, China

Received Date: 2 June 2018
Revised Date: 19 July 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475104, 21775121) and the Key Laboratory of Modern Separation Science in Shaanxi Province, China (No.17JS134).

Boronate affinity is an important method to extract selectively cis-diol biomolecules. In this work, a novel polymeric brush-type magnetic adsorbent with high density of boronate was successfully prepared. Specifically, polyethyleneimine was firstly employed to supply a large quantity of initiator sites on the surface of the magnetic nanoparticales, and then 3-acrylamidophenylboronic acid (AAPBA) monomer was grafted from the surface of magnetic nanoparticales using surface-initiated atom transfer radical polymerization (SI-ATRP). The mixture of cis-diol and non-cis-diol compounds was extracted by magnetic dispersion solid-phase extraction method, and it was found that the obtained adsorbent showed good selectivity to cis-diol small molecules and biomacromolecules. In addition, the binding capacity was measured by the adsorption isotherm method to be (151±32) mmol/g for catechol, (123±18) mmol/g for adenosine and 1.5 μmol/g for ovalbumin, respectively, which was far higher than those reported in the references. Finally the prepared nanoparticles were employed to extract four nucleosides in urine and glycoproteins in egg white, respectively. The results showed that the nanoparticles could effectively remove some potential interference in biological samples. In addition, the developed method exhibited good recovery for four nucleosides (83.8%-108.7%, RSD<15%) and excellent selectivity for glycoprotein. The adsorbents had potential in selective enrichment of cis-diol compounds from real biological samples.
- Magnetic separation,
- Boron affinity adsorbent,
- Nucleoside,
- Glycoprotein,
- Biological analysis
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沈阳化工大学材料科学与工程学院沈阳 110142