富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究

引用本文: 孙艳红, 魏佳, 王振新, 孟宪瑛. 富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究[J]. 分析化学, 2018, 46(9): 1357-1362. doi: 10.11895/j.issn.0253-3820.181293 shu

Citation: SUN Yan-Hong, WEI Jia, WANG Zhen-Xin, MENG Xian-Ying. Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1357-1362. doi: 10.11895/j.issn.0253-3820.181293 shu

富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究

1.
吉林大学白求恩第一临床医院甲状腺外科, 长春 130021;
2.
中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022
基金项目:
本文系国家自然科学基金项目（Nos.81571737，21475126）资助

摘要: 通过Au-S键将两种链长一致、序列不同的富鸟嘌呤（G）单链寡聚核苷酸PolyG₁和PolyG₂分别修饰到13 nm金纳米粒子（GNPs）表面，合成了两种单链寡聚核苷酸（ssDNAs）与GNPs复合物（PolyG₁-GNPs和PolyG₂-GNPs）。所合成的PolyG₁-GNPs和PolyG₂-GNPs在复杂分散介质中具有良好的胶体稳定性。采用紫外-可见吸收光谱、细胞透射电镜和电感耦合等离子体质谱等分析方法，考察ssDNA序列对PolyG₁-GNPs和PolyG₂-GNPs与细胞相互作用的影响。结果表明，PolyG₁-GNPs和PolyG₂-GNPs均具有较低的细胞毒性，并表现与能量相关的细胞内吞行为，ssDNA的序列决定PolyG₁-GNPs和PolyG₂-GNPs的细胞吞噬量及其在细胞内的分散状态，具有G4二级结构的PolyG₂能够显著增加细胞对其所修饰的GNPs的吞噬量和GNPs在细胞中的稳定性。

关键词:

English

Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells

1.
Department of Thyroid Surgery, the First Hospital of Jilin University, Changchun 130021, China;
2.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
Received Date: 04 May 2018
Revised Date: 27 June 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 81571737, 21475126).

Abstract: Two kinds of ssDNA-GNPs(PolyG₁-GNPs and PolyG₂-GNPs) were synthesized by modification of two different G-rich single-strand oligonucleotides(PolyG₁ and PolyG₂) onto 13-nm gold nanoparticles (GNP) surface through formation of the sulfur-gold covalent bond, respectively. The as-prepared PolyG₁-GNPs and PolyG₂-GNPs had reasonable colloidal stability in complex dispersing matrices. The effect of ssDNA sequence on the interaction between ssDNA-GNPs and cells was systematically investigated by UV-vis absorption spectroscopy, cellular transmission electronic microscopy and inductively coupled plasma mass spectrometry. The experimental results showed that both of the as-prepared PolyG₁-GNPs and PolyG₂-GNPs had low cytotoxicity and exhibited energy related endocytosis. In addition, the cellular internalization amount and dispersibility of ssDNA-GNPs were strongly affected by the sequences of immobilized ssDNA. PolyG₂ with G4 secondary structure could significantly increase the cellular internalization amount and intracellular stability of PolyG₂-GNPs.

Key words:

1. [1]
  Mirkin C A, Letsinger R L, Mucic R C, Storhoff J J. Nature,1996,38:607-609
2. [2]
  Elghanian R, Storhoff J J, Mucic R C, Letsinger R L, Mirkin C A. Science,1997,277(5329):1078-1081
3. [3]
  Rosi N L, Giljohann D A, Thaxton C S, Lytton-Jean A K R, Han M S, Mirkin C A. Science,2006,312(5776):1027-1030
4. [4]
  Rosi N L, Mirkin C A. Chem. Rev.,2005,105:1547-1562
5. [5]
  Zhao W, Ali M M, Brook M A, Li Y F. Angew. Chem. Int. Edit.,2008, 47:6330-6337
6. [6]
  Wang Z X, Ma L. Coord. Chem. Rev.,2009,253:1607-1618
7. [7]
  Chen N, Li J, Song H Y, Chao J, Huang Q, Fan C H. Acc. Chem. Res.,2014,47(6):1720-1730
8. [8]
  Koo K M, Sina A A, Carrascosa L G, Shiddiky M J A, Trau M. Anal. Methods,2015,7:7042-7054
9. [9]
  Qin L, Zeng G, Lai C, Huang D, Xu P, Zhang C, Cheng M, Liu X, Liu S, Li B, Yi H.Coord. Chem. Rev.,2018,359:1-31
10. [10]
  LI Zhu-Heng, MA Li-Na, LIU Dian-Jun,WANG Zhen-Xin. Chinese J. Appl. Chem.,2016,33(9):1009-1016 李铸衡, 马立娜, 刘殿骏, 王振新.应用化学,2016,33(9):1009-1016
11. [11]
  Charbgoo F, Nejabat M, Abnous K, Soltani F, Taghdisi S M, Alibolandi M, Shier W T, Steele T W J, Ramezani M. J. Controlled Release,2018,272:39-53
12. [12]
  Halo T L, McMahon K M,Angeloni N L, Xu Y, Wang W, Chinen A B, Malin D, Strekalova E, Cryns V L, Cheng C, Mirkin C A, Thaxton C S. Proc. Natl. Acad. Sci. USA,2014,111(48):17104-17109
13. [13]
  Randeria P S, Jones M R, Kohlstedt K L, Banga R J, de la Cruz M O, Schatz G C, Mirkin C A. J. Am. Chem. Soc.,2015,137(10):3486-3489
14. [14]
  Zhao X J, Hilliard L R,Mechery S J, Wang Y P, Bagwe R P, Jin S G, Tan W H. Proc. Natl. Acad. Sci. USA,2004,101(42):15027-15032
15. [15]
  Choi C H J,Hao L, Narayan S P, Auyeung E, Mirkin C A. Proc. Natl. Acad. Sci. USA,2013,110(19):7625-7630
16. [16]
  Narayan S P, Choi C H J, Hao L, Calabrese C M, Auyeung E, Zhang C, Goor O J G M, Mirkin C A. Small,2015,11(33):4173-4182
17. [17]
  Rosi N L, Mirkin C A. Chem. Rev.,2005,105(4):1547-1562
18. [18]
  Li J, Huang J, Yang X, Yang Y,Quan K, Xie N, Wu Y, Ma C, Wang K. Talanta,2018,183:11-17
19. [19]
  Paramasivan S, Rujan I, Bolton P H. Methods,2007,43(4):324-331
20. [20]
  Kuznetsov N V. Methods Mol. Biol.,2013,986:41-56
21. [21]
  Kumar S, Aaron J,Sokolov K. Nat. Protoc.,2008,3(2):314-320
22. [22]
  Zheng D,Giljohann D A, Chen D L, Massich M D, Wang X Q, Iordanov H, Mirkin C A, Paller A S. Proc. Natl. Acad. Sci. USA,2012,109(30):11975-11980

扫一扫看文章

计量

PDF下载量: 8
文章访问数: 874
HTML全文浏览量: 48

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究

English

Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究

English

Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs