Citation: CHEN Chuan-Xia, NI Peng-Juan, JIANG Yuan-Yuan, ZHAO Zhen-Lu, LU Yi-Zhong. Dual-mode Detection of Dopamine Based on Enhanced Fluorescent and Colorimetric Signals of Fe³⁺-H₂O₂-o-Phenylenediamine System[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(8): 1231-1237. doi: 10.11895/j.issn.0253-3820.181195 shu

Dual-mode Detection of Dopamine Based on Enhanced Fluorescent and Colorimetric Signals of Fe³⁺-H₂O₂-o-Phenylenediamine System

School of Materials Science and Engineering, University of Jinan, Jinan 250022, China

Received Date: 24 March 2018
Revised Date: 24 April 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21705056) and the Natural Science Foundation of Shandong Province, China (Nos. ZR2018BB057, ZR2018PB009, ZR2017MB022).

A dual-mode "light-on" assay platform based on enhanced fluorescent and colorimetric signals of Fe³⁺-H₂O₂-OPD system for detection of dopamine was designed. Fe³⁺ can catalyze the H₂O₂-mediated oxidation of colorless and nonfluorescent o-phenylenediamine (OPD), and the resultant of 2,3-diaminophenazine (DAP) exhibits a visible yellow color, emitting a yellow fluorescence. However, the reaction rate is extremely slow. When dopamine is introduced, it involves a Fenton type reaction which generates hydroxyl radical (·OH) continuously, thus increasing the conversion rate of OPD to DAP. Correspondingly, both color and fluorescence of the sensing system are enhanced obviously. On the basis of this fact, a sensor with dual-readout for the detection of dopamine is established via measuring the fluorescent and colorimetric signals of the Fe³⁺-H₂O₂-OPD system. The linear ranges are 0.05-20 μ mol/L and 0.10-18 μ mol/L, and the detection limits are calculated to be 15 and 65 nmol/L (S/N=3) for fluorescent and colorimetric detection, respectively. The fluorescent and colorimetric dual-readout method developed in the present work features simplicity, high sensitivity and visualization, and enhances the detection accuracy of dopamine. Moreover, the method has been successfully applied to detection of dopamine in human urine with satisfactory results.
- Dopamine,
- Fluorescent,
- Colorimetric,
- Fenton reaction,
- Hydroxyl radical
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Dual-mode Detection of Dopamine Based on Enhanced Fluorescent and Colorimetric Signals of Fe3+-H2O2-o-Phenylenediamine System

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通讯作者: 陈斌, bchen63@163.com

Dual-mode Detection of Dopamine Based on Enhanced Fluorescent and Colorimetric Signals of Fe³⁺-H₂O₂-o-Phenylenediamine System