Citation: DI Wei, ZHANG Ying-Chun, YI Hua-Xi, HAN Xue, WANG Shu-Mei, ZHANG Lan-Wei. Research Methods for Structural Analysis of Lactic Acid Bacteria Induced Exopolysaccharides[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 875-882,924. doi: 10.11895/j.issn.0253-3820.181178 shu

Research Methods for Structural Analysis of Lactic Acid Bacteria Induced Exopolysaccharides

1.
School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150090, China;
2.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China;
3.
School of Food Science and Engineering, Harbin University, Harbin 150086, China

Received Date: 21 March 2018
Revised Date: 8 April 2018

Fund Project: This work was supported by the Natural Science Foundation of Heilongjiang Province, China (No.C201433).

Exopolysaccharides (EPSs), produced by lactic acid bacteria (LAB), have been used primarily to improve the quality and taste of food, also possess a variety of unique biological functions, such as immunoregulation and anti-tumor activities. The diversity of molecular structural characteristics of LAB-generated EPSs represents one of the main factors responsible for this plethora of functions. Accordingly, the structural analysis of the EPSs produced by LAB is both a prerequisite and basis for examining its functional and structure-activity relationships. In this article, we summarized the current progress of key methodologies involved in the structural analysis of LAB-generated EPSs, including their isolation, purification, primary structure and advances in structural research. A comprehensive discussion regarding the application of chemical analysis, instrument analysis and computer aided technology in the structure analysis of LAB-generated EPSs was provided. Further, the future development of the research on the structure of LAB-generated EPSs was presented.
- Lactic acid bacteria,
- Exopolysaccharides,
- Isolation and purification,
- Structure analysis,
- Review
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沈阳化工大学材料科学与工程学院沈阳 110142