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Citation: WANG Hua-Jie, LIU Yuan-Min, LI Guang-Yu, WU Zhi-Yong. Study on Interaction of Protamine and Single-stranded DNA by Current Pulses Through Single Nanopore[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(6): 851-857. doi: 10.11895/j.issn.0253-3820.171416 shu

Study on Interaction of Protamine and Single-stranded DNA by Current Pulses Through Single Nanopore

Research Center for Analytical Sciences(RCAS), Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China

Received Date: 12 November 2017
Revised Date: 13 March 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21575019) and Liaoning Provincial Science Foundation (No. LZ2015036).

Single nanopore current pulse method is a new, rapid and simple detection method, which is promising for single-molecule DNA sequencing and bio-sensing. Due to the short duration and the low current amplitude of the pulses caused by molecular translocation under normal conditions, pulse detection system with fast response and high sensitivity is required. In this work, based on a lab-established pulse detection system, the effect of protamine in the regulation of single-stranded (ssDNA) current pulses with α-hemolysin (α-HL) single nanopore interface was investigated. Experimental results showed that the pulses positive charged protamine and negative ssDNA probes were both well observed with the established system, and both the pulse amplitude and duration of ssDNA were increased as a result of interaction with protamine. This study provides a way to improve the resolving power of current pulses based on molecular interactions.
- α-Hemolysin,
- Protamine,
- Single-stranded DNA,
- Interaction
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