Citation: SHAO Shuang, TANG Yu-Jiao, DAI Shi-Yan, ZHOU Yu-Ting, CHENG Gui-Fang, HE Pin-Gang, FANG Yu-Zhi. A Highly Sensitive Electrochemical Biosensor for Detection of Adenosine Triphosphate Based on Space Block[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(2): 254-259. doi: 10.11895/j.issn.0253-3820.171382 shu

A Highly Sensitive Electrochemical Biosensor for Detection of Adenosine Triphosphate Based on Space Block

College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Received Date: 2 November 2017
Revised Date: 8 December 2017

Fund Project: This work was supported by the Nation Key Foundation for Exploring Scientific Instrument (No.2011YQ150078), the Science and Technology Commission of Shanghai (No.15142200600), and the National Natural Science Foundation of China (No.21575024).

In this work, a highly sensitive electrochemical biosensor for the detection of trace adenosine triphosphate (ATP) was proposed. The biosensor was based on porous anodic alumina (PAA) and SiO₂ nanoparticles combining with several oligonucleotides to construct sandwich structure. It was characterized by scanning electron microscopy, fluorescence microscopy, differential pulse voltammetry and electrochemical impedance spectroscopy, which conformed to the reliability of the biosensor fabrication and the feasibility of the detection. In the presence of ATP, the sandwich structures could be destroyed. The variation of the current was directly corresponding to the amount of the ATP. The application of SiO₂ nanoparticles could effectively reduce the background and increase the sensitivity of the biosensor. The calibration curve of ATP was obtained in the range of 0.025-0.900 nmol/L with the detection limit of 13 pmol/L (S/N=3). Also, the biosensor exhibited a good specificity. Besides, the sensor was constructed easily and possessed excellent regeneration ability. The proposed biosensor was applied in detection of real sample such as mice blood. Therefore, the proposed ATP-sensing biosensor could be expected to be applied in clinical, pharmaceutical and environmental detection.
- Adenosine triphosphate,
- Porous anodic alumina,
- Silicon dioxide nanoparticles,
- Biosensors
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