Citation: LIU Xiao-Hui, WANG Ze-Cheng, ZHANG Xiao-Bing, XU Dan-Ke. Screening of Lomefloxacin Aptamers Based on Polydopamine Nanospheres[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1971-1979. doi: 10.11895/j.issn.0253-3820.171319 shu

Screening of Lomefloxacin Aptamers Based on Polydopamine Nanospheres

1.
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China
2.
State Key Laboratory of Chemo/Biosensing and Chemometrics, School of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Corresponding author: ZHANG Xiao-Bing, xbzhang@hnu.edu.cn XU Dan-Ke, xudanke@nju.edu.cn
Received Date: 10 October 2017
Accepted Date: 8 November 2017

Fund Project: the National Natural Science Foundation of China 21227009the National Natural Science Foundation of China 21775068the National Natural Science Foundation of China 21475060the National Science Fund for Creative Research Groups 21121091the National Natural Science Foundation of China 21405077This work was supported by the National Natural Science Foundation of China (Nos. 21775068, 21227009, 21475060, 21405077), and the National Science Fund for Creative Research Groups (No.21121091)

Figures(6)

Screening aptamers using nano-materials (such as graphene oxide, gold nanoparticle, carbon nano-tube, etc.) that can quench fluorescence and absorb single stranded DNA using hydrogen bond, π-π bond, charge transfer, and other non-covalent ways to combine with ssDNA, but without other conformational DNA, can excellently separate specific aptamers from non-specific ones. In this case, we can shorten the cycle numbers, enhance the success rate, and reduce the labour intensity of systematic evolution of ligands by exponential enrichment (SELEX). Especially for small molecular target, due to its difficulty in immobilization and small size, it is difficult to use traditional methods such as SPR-SELEX or affinity-SELEX for screening. In this experiment, polydopamine nanospheres (MNPs@PDAs) were used to screen the Lomefloxacin. Also, we used magnetic separation technique to screen small molecular target rapidly. The interaction between aptamer candidates and the target could be monitored by recovery ratio of ssDNA and the whole MNPs@PDAs-SELEX process was performed through seven-round selection. As a result, we successfully obtained the aptamer named AF-3 which could recognize the lomefloxacin with high affinity (K_D=(17.57±0.5) nmol/L). This screening method based on MNPs@PDAs makes it a promising reagent in the efficient aptamers selection of other targets.
- Aptamer,
- Systematic evolution of ligands by exponential enrichment,
- Polydopamine,
- Lomefloxacin
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沈阳化工大学材料科学与工程学院沈阳 110142