Citation: WANG Xiao-Yu, CHEN Xiao-Fei, GU Yan-Qiu, CAO Yan, YUAN Yong-Fang, HONG Zhan-Ying, CHAI Yi-Feng. Progress of Cell Membrane Chromatography and Its Application in Screening Active Ingredients of Traditional Chinese Medicine[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(11): 1695-1702. doi: 10.11895/j.issn.0253-3820.171287 shu

Progress of Cell Membrane Chromatography and Its Application in Screening Active Ingredients of Traditional Chinese Medicine

¹ East School of Pharmacy, Second Military Medical University, Shanghai 200433, China;
² Department of Pharmacy, Shanghai 9th People's Hospital, Shanghai 201999, China

Received Date: 2 October 2017
Revised Date: 2 August 2018

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 81503039, 81573396, 81673386) and the "Yang-Fan Project" of Science and Technology Commission of Shanghai Municipality, China (No.15YF1400200).

Cell membrane chromatography, which has been used to build an in vitro screening model, has become a practical method to screen active ingredients of traditional Chinese medicine or to study the interaction between drugs and receptors. In this paper, the development of cell membrane chromatography technology and its preparation methods, the optimization of the system and methodological for its stability and other issues were reviewed, as well its unique advantages on selection of active ingredients of traditional Chinese medicine. On this basis, the future development trend of cell membrane chromatography technology was discussed in order to provide ideas for the further development of cell membrane chromatography and its further application in the field of drug high throughput screening.
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