Citation: LIU Yang, ZOU Xiao-Ran, LI Chen-Chen, ZHANG Chun-Yang. Recent Advances in Sensitive Detection of Kinases[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(1): 11-19. doi: 10.11895/j.issn.0253-3820.171275 shu

Recent Advances in Sensitive Detection of Kinases

College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China

Received Date: 27 September 2017
Revised Date: 7 November 2017

Fund Project: This work was supported by the National Science Foundation for Distinguished Young Scholars of China (No.21325523).

Kinases are a class of enzymes that catalyze the transfer of a phosphate group from a high-energy molecule to their substrates (i.e., phosphorylation). Kinase-induced intracellular phosphorylation plays important roles in a variety of cellular processes including metabolism, cell signaling, protein regulation, DNA replication and repair. Consequently, kinases have become potential biomarkers for disease diagnosis and drug discovery, and the development of simple and sensitive methods for kinase assay is highly desirable. In this review, we summarize the recent advances in kinase assays with protein kinase A (PKA), casein kinase-2 (CKⅡ) and T4 polynucleotide kinase (T4 PNK) as the models. We focus on the newly emerging methods for kinase assays including fluorescent, single-molecule detection, colorimetric, chemiluminescent, bioluminescent, and electrochemical and photoelectrochemical methods. Furthermore, we give a new insight into the future direction of kinase assays.
- Kinases,
- Sensitive detection,
- Analytical methods,
- Review
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