Citation: LIU Cheng-Xin, XIAO Zhi-Ming, JIA Zheng, TIAN Jing, LIU Xiao-Lu, FAN Xia. Quantitative Determination of Arsenic Species in Feed Using Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(4): 537-542. doi: 10.11895/j.issn.0253-3820.171228 shu

Quantitative Determination of Arsenic Species in Feed Using Liquid Chromatography-Hydride Generation Atomic Fluorescence Spectrometry

China National Feed Quality Control Center, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received Date: 4 September 2017
Revised Date: 4 February 2018

Fund Project: This work was supported by the National Key Research and Development Program of China during the Thirteenth Five-year Plan Period.

A sensitive and rapid method using liquid chromatography-hydride generation atomic fluorescence spectrometry (HPLC-HG-AFS) was developed for the simultaneous determination of seven arsenic species As³⁺, As⁵⁺, MMA, DMA, p-ASA, 4-OH and ROX in feeds. The isolation of the analytes from feed samples was accomplished using methanol water (1:1, V/V). The target compounds were separated on a PRP-X100 anion exchange column and then analyzed by HG-AFS. The mobile phase was 15 mmol/L (NH₄)₂HPO₄ and 10 mmol/L potassium acid phthalate. Good linearity was obtained for all of the seven arsenic species, with linear coefficients higher than 0.9964. The LODs of the seven arsenic species were between 5 and 30 μg/kg. Average recoveries for the seven analytes were in the ranges of 76.3%-108.1%, with intra-and inter-day repeatability lower than 7.7% and 17.4%, respectively. This validated method was successively applied to the determination of arsenic species in feed. This method was sensitive, simple, cheap and low operation cost, and could be used for the determination of the arsenicspecies in feeds.
- Liquid chromatography,
- Hydride generation-atomic fluorescence spectrometry,
- Feed,
- Arsenic,
- Species analysis
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
24. [24]
25. [25]
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