一种黄酮类荧光探针的合成及用于肼的检测

引用本文: 张勇, 刘劲风, 易润豪, 艾思凡, 成焕仁, 贾文志. 一种黄酮类荧光探针的合成及用于肼的检测[J]. 分析化学, 2018, 46(4): 511-516. doi: 10.11895/j.issn.0253-3820.171222 shu

Citation: ZHANG Yong, LIU Jin-Feng, YI Run-Hao, AI Si-Fan, CHENG Huan-Ren, JIA Wen-Zhi. Synthesis and Application of A Flavone-based Fluorescent Probe for Detection of Hydrazine[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 511-516. doi: 10.11895/j.issn.0253-3820.171222 shu

一种黄酮类荧光探针的合成及用于肼的检测

张勇 ^1, ,
刘劲风 ^1, ,
易润豪 ^1, ,
艾思凡 ^2, ,
成焕仁 ^1, ,
贾文志 ^1,

1.
湖北理工学院化学与化工学院, 黄石 435003;
2.
天津科技大学理学院, 天津 300457
基金项目:
本文系国家自然科学基金项目（No.21603069）、湖北省教育厅高校青年教师深入企业行动计划（No.XD2014677）和湖北理工学院大学生科技创新专项研究项目（No.17cx08）资助

摘要: 以3-羟基黄酮为荧光团，设计合成了一种用于检测肼的荧光增强型探针ZY8，研究了其光谱性质。结果表明，在Tris-HCl/乙醇溶液（9∶1，V/V，pH 7.40）中，ZY8对N₂H₄具有较好的选择性和专一性。在1.6×10^-7~6.2×10^-5 mol/L浓度范围内，N₂H₄的浓度与ZY8的荧光强度呈现良好的线性关系，检出限为1.6×10^-7 mol/L。ZY8自身荧光微弱，向其中加入N₂H₄后，荧光增强9倍左右，而且在波长365 nm的紫外光照射下，溶液的颜色由浅灰绿色变成了明亮的草绿色，可实现对N₂H₄的可视化识别。它可在接近生理pH范围内对N₂H₄进行检测，响应快，抗干扰能力强。ZY8制成的试纸能裸眼检测低于mmol/L级别的N₂H₄溶液，将其应用于不同水样中N₂H₄的检测，N₂H₄的回收率在96.0%-104.2%之间，相对标准偏差（RSD）小于4%，表明ZY8在环境污染监测领域有潜在的应用价值。

关键词:

肼
/ 荧光探针
/ 3-羟基黄酮

English

Synthesis and Application of A Flavone-based Fluorescent Probe for Detection of Hydrazine

1.
College of Chemistry and Chemical Engineering, Hubei Polytechnic University, Huangshi 435003, China;
2.
College of Science, Tianjin University of Science and Technology, Tianjin 300457, China
Received Date: 04 September 2017
Revised Date: 26 January 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21603069), Young College Teachers' Entering into Enterprises Program of Hubei Provincial Department of Education (No.XD2014677) and College Students' Science and Technology Innovation Project of Hubei Polytechnic University(No.17cx08).

Abstract: A fluorescence enhancement probe (ZY8) for the detection of N₂H₄ was designed and synthesized by employing 3-hydroxyflavone as a fluorophore, and its spectral properties had been investigated. The results showed that ZY8 had relatively good selectivity and specificity to N₂H₄ in Tris-HCl-ethanol solution (9:1, V/V, pH 7.40). The fluorescent intensity of ZY8 exhibited good linear relationship with concentration of N₂H₄ in the concentration range of 1.6×10^-7 mol/L-6.2×10^-5 mol/L, and its detection limit was estimated to be 1.6×10^-7 mol/L. ZY8 itself had weak fluorescence, upon addition of N₂H₄, an approximate 9-fold fluorescence enhancement was observed, and the color of the solution changed from light grayish green to bright grass-green at UV light of 365 nm. So ZY8 might be used to the visual recognition of N₂H₄. ZY8 could detect N₂H₄in near-physiological pH range, and it had fast response and strong anti-interference ability. Moreover, ZY8 could be loaded as test paper for naked-eye detection of N₂H₄ at mmol/L level in water solution, and it was also applied in the determination of N₂H₄ in various water samples by the standard addition recovery experiments, with the recovery ratio ranged from 96.0% to 104.2%%, and RSD of all < 4%. The results of this study demonstrated that ZY8 had potential application to the detection of N₂H₄ in the monitoring of environmental pollution.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

一种黄酮类荧光探针的合成及用于肼的检测

English

Synthesis and Application of A Flavone-based Fluorescent Probe for Detection of Hydrazine

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

一种黄酮类荧光探针的合成及用于肼的检测

English

Synthesis and Application of A Flavone-based Fluorescent Probe for Detection of Hydrazine

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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