Citation: TANG Dan-Dan, ZHANG Jin-Yi, HOU Xian-Deng, WU Peng. Phosphorescent Inner Filter Effect-based Sensing System for Determination of β-Glucuronidase Using Manganese-doped Zinc Sulfide Quantum Dots[J]. Chinese Journal of Analytical Chemistry, ;2017, 45(12): 1909-1914. doi: 10.11895/j.issn.0253-3820.171216 shu

Phosphorescent Inner Filter Effect-based Sensing System for Determination of β-Glucuronidase Using Manganese-doped Zinc Sulfide Quantum Dots

TANG Dan-Dan ¹ ,
ZHANG Jin-Yi ¹ ,
HOU Xian-Deng ^1,2,, ,
WU Peng ^2,,

1.
College of Chemistry, Sichuan University, Chengdu 610064, China
2.
Analytical & Testing Center, Sichuan University, Chengdu 610064, China

Corresponding author: HOU Xian-Deng, houxd@scu.edu.cn WU Peng, wupeng@scu.edu.cn
Received Date: 8 September 2017
Accepted Date: 19 October 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21475090)the National Natural Science Foundation of China 21475090

Figures(6)

The key to maximize the sensitivity of inner filter effect (IFE)-based assay is to enlarge the overlap between the absorption spectra of the absorber and the excitation or emission spectra of the fluorophore. In this work, Mn-doped ZnS quantum dots (QDs) were chosen for IFE-based detection of β-glucuronidase (GUS), since the excitation of QDs was perfectly overlapped with the absorption of the substrate of GUS, namely 4-nitrophenyl-β-D-glucuronide (PNPG). In addition, the phosphorescence emission from Mn-doped ZnS QDs could eliminate the fluorescence background from biological samples. Therefore, a simple turn-on phosphorescent GUS assay was developed, with the linear range of 10-300 U/L and limit of detection of 7 U/L (S/N=3).
- β-Glucuronidase,
- Manganese-doped zinc sulfide quantum dots,
- Inner-filter effect,
- Escherichia coli
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