Citation: TANG Tao, ZHANG Wei-Bing, ZHANG Zheng, XIA Ming-Zhu, GONG Xue-Dong, WANG Feng-Yun, LI Tong. Design and Evaluation of Online/offline Interface Based on Double Loop for Two-dimensional Chromatography[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(3): 311-316. doi: 10.11895/j.issn.0253-3820.171182 shu

Design and Evaluation of Online/offline Interface Based on Double Loop for Two-dimensional Chromatography

1.
Institute of Industrial Chemistry, Nanjing University of Science and Technology, Nanjing 210094, China;
2.
College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China;
3.
Dalian Elite Analytical Instruments CO., Ltd. Dalian 116023, China

Received Date: 2 November 2017
Revised Date: 8 December 2017

Fund Project: This work was supported by the National Major Scientific Instruments and Equipments Special Project of China (No. 2012YQ12004401), and the National Natural Science Foundation of China (No. 51472121).

By using a double loop technique based on a set of mechanical transmission components and a flow path system, a double function of injection and fraction collection was realized. On the basis of double loop technique, a novel type of online/offline interface for two-dimensional liquid chromatography was designed to construct an efficient separation system coupling two different separation modes with a higher peak capacity, and the functions of the interface were evaluated. By means of connecting the interface to an HPLC system, the multiple functions of injection, fraction collection and injection again were fulfilled for preparation and purity analysis of 4 kinds of aromatic compounds. As for combination of 2 sets of HPLC system, 5 kinds of protein samples were separated preliminarily by strong cation exchange chromatography, and the components difficult to separate were collected and injected into reversed phase chromatographic system for further separation. Furthermore, the interface was applied to coupling two chromatographic systems in both strong cation exchange mode and microcolumn reversed-phase mode for the two-dimensional separation of bovine serum albumin enzymatic digest. When 1 mAU was set as the integral threshold, a total number of 292 peaks were identified. With the help of the online/offline interface, the preparation of microscale samples, fine separation of hardly separated samples and two-dimensional separation of complex samples were achieved flexibly. The result indicated that the system was a potent tool for the construction of two-dimensional chromatographic system and separation research.
- Double loop,
- Online interface,
- Offline interface,
- Fraction collection,
- Auto sampler,
- Two-dimensional liquid chromatography
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沈阳化工大学材料科学与工程学院沈阳 110142