Citation: HUANG Yuan-Fei, LOU Xiao-Yi, ZHOU Zhe, WANG Yang, KONG Cong, HUANG Dong-Mei, CAI You-Qiong, Yu Hui-Juan. Determination of 11 Kinds of Aminoglycosides in Aquatic Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Molecularly Imprinted Polymers Solid Phase Extraction[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(3): 454-461. doi: 10.11895/j.issn.0253-3820.171175 shu

Determination of 11 Kinds of Aminoglycosides in Aquatic Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Molecularly Imprinted Polymers Solid Phase Extraction

1.
Laboratory of Risk Assessment in Aquatic products of Ministry of Agriculture, East China Sea Fisheries Research Institute, Shanghai 200090, China;
2.
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China;
3.
Thermo Fisher Scientific Inc., Shanghai 201206, China

Received Date: 17 August 2017
Revised Date: 25 December 2017

Fund Project: This work was supported by the Shanghai Municipal Agricultural Commission (No. (2016)No.1-4-1).

An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed for the determination of 11 kinds of aminoglycosides (AGs), including paromomycin, spectinomycin, tobramycin, gentamycin, kanamycin, hygromycin B, apramycin, streptomycin, dihydrostreptomycin, amikacin and neomycin in aquatic products. Samples were extracted by phosphate buffer solution, and purified on molecularly imprinted polymers (MIP) solid phase extraction column. After separated by Obelisc R chromatographic column, AGs were detected by UPLC-MS/MS. It showed a good linearity relationship in the AGs concentration range of 1.0-1000 ng/mL with the correlation coefficient R²>0.994. The limit of detection (LOD, S/N ≥ 3) was ranged from 1.0 μg/kg to 10.0 μg/kg, and the limit of quantitation (LOQ, S/N ≥ 10) was ranged from 2.0 μg/kg to 20.0 μg/kg. Besides, the average recoveries presented 78.4%-109.6% with the relative standard deviation (RSD, n=6) of 2.3%-14.9%. This method was successfully applied to the simultaneous determination of 11 kinds of AGs with high sensitivity in aquatic products.
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沈阳化工大学材料科学与工程学院沈阳 110142