Citation: BAI Min, CAO Xiao-Wen, CHEN Feng, ZHAO Yue, ZHAO Yong-Xi. A Förster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(1): 39-47. doi: 10.11895/j.issn.0253-3820.171163 shu

A Förster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells

Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China

Received Date: 10 August 2017
Revised Date: 7 September 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (Nos. 21475102, 31671013) and the "Young Talent Support Plan" of Xi'an Jiaotong University, China.

Hydrogen sulfide (H₂S) has been confirmed as a significant endogenous gaseous signaling molecule involved in various physiological processes. To monitor H₂S in living cells, a Förster resonance energy transfer (FRET) ratiometric probe based on quantum dot-cresyl violet was developed. In this work, quantum dot nanospheres (QDS) were firstly synthesized via a facile ultrasonication emulsion strategy, and the mixture chloroform solution containing hydrophobic quantum dots and COOH-functionalized amphiphilic polymer were successfully transferred into the oil-in-water micelle. The negatively charged quantum dot nanospheres with quantum dots embedded in the polymer matrixes were successfully fabricated after the evaporation of chloroform. And then, these quantum dot nanospheres were condensed with positively charged cresyl violet-azide (CV-N₃) via electrostatic interaction to obtain the QDS-N₃ complexes. The as-prepared QDS-N₃ complexes were monodispersed nanospheres with an average diameter of about 120 nm. These complexes were taken up by the cell through endocytosis, and they were still stable even in wide pH range. In addition, the QDS-N₃ complexes exhibited no cellular toxicity which was verified by MTT assay. In this ratiometric probe, CV-N₃ as a FRET acceptor was conjugated to quantum dot nanospheres. The quantum dots emitted at 591 nm and served as the FRET donor; once the aryl azide on the CV-N₃ was reduced to aniline by H₂S, the probe emitted at 620 nm. The ratiometric probe allowed the elimination of interference of excitation intensity, intracellular environment and other factors. Furthermore, this method also offered a general protocol for preparing nanosensors for monitoring various small molecular in living cells.
- Quantum dot,
- Cresyl violet,
- Ratiometric fluorescence,
- Cell imaging,
- Hydrogen sulfide
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