电喷雾离子迁移谱检测氨基酸及多肽的研究

引用本文: 孔景临, 刘卫卫, 丁俊杰, 张琳, 李宝强, 秦墨林, 陈创, 李海洋. 电喷雾离子迁移谱检测氨基酸及多肽的研究[J]. 分析化学, 2017, 45(11): 1583-1588. doi: 10.11895/j.issn.0253-3820.171052 shu

Citation: KONG Jing-Lin, LIU Wei-Wei, DING Jun-Jie, ZHANG Lin, LI Bao-Qiang, QIN Mo-Lin, CHEN Chuang, LI Hai-Yang. Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1583-1588. doi: 10.11895/j.issn.0253-3820.171052 shu

电喷雾离子迁移谱检测氨基酸及多肽的研究

孔景临 ^1, ,
刘卫卫 ^1, ,
丁俊杰 ^1, ,
张琳 ^1, ,
李宝强 ^1, ,
秦墨林 ^1, ,
陈创 ^2, ,
李海洋 ^2,

1.
防化研究院, 北京 102205;
2.
中国科学院大连化学物理研究所, 大连 116023

摘要: 建立了氨基酸及多肽的电喷雾离子迁移谱检测方法。采用自制的电喷雾离子迁移谱装置，在室温条件下以甲醇为溶剂，空气为漂移气体，流速为1000 mL/min，电喷液流速为2μL/min，测试了甘氨酸、胱氨酸、组氨酸、精氨酸4种氨基酸及缓激肽片段（1~7）和P物质2种多肽的离子迁移谱，计算出上述化合物的约化迁移率。离子迁移谱图反映出化合物的结构信息，具有指纹谱特征。此装置在1 min的检测时间内对P物质的检测灵敏度达到855 ng/mL。结果表明，电喷雾离子迁移谱可用于氨基酸及多肽类化合物的现场快速鉴定。

关键词:

English

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

1.
Institute of Chemical Defense, Beijing 102205, China;
2.
Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian 116023, China
Received Date: 30 June 2017
Revised Date: 08 September 2017

Abstract: The electrospray ionization-ion mobility spectrometric (ESI-IMS) technique has the potential as an analytical separation tool in analyzing polypeptides and amino acids for fast screening unknown samples in anti-chemical and biological terror attacks. A method for detecting several polypeptides and amino acids was developed based on ESI-IMS using air as drift gas at room temperature. The ion mobility of four amino acids and two polypeptides dissolved in methanol was determined on the system at elution rate of 2μL/min. The spectra of these compounds had characteristics of finger-printing maps. The limit of detection of this instrument for Substance P could reach 855 ng/mL in 1 min. The results showed that a small, self-contained ESI-IMS instrument with reservoirs of air could be used to quickly detect and accurately identify polypeptides and amino acids.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

电喷雾离子迁移谱检测氨基酸及多肽的研究

English

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

电喷雾离子迁移谱检测氨基酸及多肽的研究

English

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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