Home

Citation: DENG Wen-Chan, HAN Guo-Bin, LI Yuan-Fang, HUANG Cheng-Zhi. Distinction of Cells Infected with Respiratory Syncytial Virus by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2018, 46(2): 165-169. doi: 10.11895/j.issn.0253-3820.171011 shu

Distinction of Cells Infected with Respiratory Syncytial Virus by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

1.
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China;
2.
College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China

Received Date: 16 June 2017
Revised Date: 12 December 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (No.21535006).

Respiratory syncytial virus (RSV), which is a major cause of lower respiratory tract infections during infancy and childhood, is also an important pathogen for immunosuppression in elder and diseased areas. In order to research the pathogenesis of RSV, the matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS)was used to explore the difference between uninfected cells and virus-infected cells. The result showed that, in the molecular weight range of 5000~10000 Da, there are three component peaks expressed with significant difference in both normal group and infection group. One of the three components was up-regulated (m/z 6154.25) and the other two were down-regulated (m/z 7658.47 and 9259.82) after RSV infection. This result proved the obvious differences between the infected cells and untreated cells.The differential expression may provide a feasible method for RSV disease diagnosis and pathology research, and also provide scientific evidences for research on development of RSV therapeutic drugs.
- Matrix-assisted laser desorption/ionization mass spectrometry,
- Respiratory syncytial virus,
- Biomarker
1. [1]
2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
1. [1]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
2. [2]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
3. [3]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
4. [4]
  Shengwen XU , Longlong YANG , Houji CAO , Deshuang TU , Xing WEI , Changsheng LU , Hong YAN . Research progress on light-induced functionalization of polyhedral carborane clusters. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2187-2200. doi: 10.11862/CJIC.20250192
5. [5]
  Qin Hou , Jiayi Hou , Aiju Shi , Xingliang Xu , Yuanhong Zhang , Yijing Li , Juying Hou , Yanfang Wang . Preparation of Cuprous Iodide Coordination Polymer and Fluorescent Detection of Nitrite: A Comprehensive Chemical Design Experiment. University Chemistry, 2024, 39(8): 221-229. doi: 10.3866/PKU.DXHX202312056
6. [6]
  Yuai Duan , Xuanyu Gan , Yao Fu , Yingjie Cao , Hongliang Han , Zhanfang Ma . Application and Innovative Design of Digital Technology in the Preparation Experiment of Cis(Trans)-Diglycine Copper Complexes. University Chemistry, 2026, 41(1): 373-381. doi: 10.12461/PKU.DXHX202504048
7. [7]
  Zhike Yang , Jinfan Xu , Junhao Chen , Zheng Yang , Fei Ding , Neil Qiang Su . AI NMR Assistant: A DP5-Based Intelligent System for NMR Spectral Interpretation. University Chemistry, 2026, 41(1): 20-28. doi: 10.12461/PKU.DXHX202506013
8. [8]
  Siwei Hou , Yaxin Niu , Guanglu Zhang , Yanmei Yang , Xu Wang , Zhenzhen Chen . Application of Solid-Phase Microextraction and Mass Spectrometry in Environmental Detection. University Chemistry, 2026, 41(3): 297-306. doi: 10.12461/PKU.DXHX202504078
9. [9]
  Zufeng Qiu , Jie Ouyang , Yiru Wang , Hengting Yang , Xin Liao , Chi Zhang , Xuanyao Jiang , Shunliu Deng , Zhiwei Lin . 综合运用分析仪器解析“盲盒”样品——未知物的剖析. University Chemistry, 2025, 40(6): 296-302. doi: 10.12461/PKU.DXHX202405167
10. [10]
  Chenyang WANG , Yiyan BAI , Wei ZHANG , Zhaorong LIU , Yuchun WANG . Performance of photo-assisted copper oxide catalyzed hydrolysis of ammonia borane to produce hydrogen. Chinese Journal of Inorganic Chemistry, 2026, 42(1): 97-110. doi: 10.11862/CJIC.20250116
11. [11]
  Zhen Zhang , Jie Liu , Hongwei Kang , Cuibing Bai , Jitang Chen . Exploration of 3D Printing-Assisted Teaching of Chemical Engineering Principles Driven by New Quality Productive Forces. University Chemistry, 2026, 41(2): 21-27. doi: 10.12461/PKU.DXHX202501021
12. [12]
  Hanmei Lü , Xin Chen , Qifu Sun , Ning Zhao , Xiangxin Guo . Uniform Garnet Nanoparticle Dispersion in Composite Polymer Electrolytes. Acta Physico-Chimica Sinica, 2024, 40(3): 2305016-0. doi: 10.3866/PKU.WHXB202305016
13. [13]
  Wenke ZHENG , Ce LIU , Wei CHEN , Hongshan KE , Fanlong ZENG , Yibo LEI , Anyang LI , Wenyuan WANG . Synthesis and bonding analysis of low-coordinate Fe and Cr complexes with ultra-bulky silylamino groups. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1285-1293. doi: 10.11862/CJIC.20250095
14. [14]
  Changsheng An , Tao Liu . Decoding SEI chemistry at the lithium-metal potential. Acta Physico-Chimica Sinica, 2025, 41(9): 100101-0. doi: 10.1016/j.actphy.2025.100101
15. [15]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
16. [16]
  Zhijun Huang , Jiawei Li , Mojin Lu , Fa Zhou , Limiao Chen , Jianhan Huang , Younian Liu . Spying Operation of the Rabies Virus. University Chemistry, 2024, 39(9): 164-169. doi: 10.12461/PKU.DXHX202403026
17. [17]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
18. [18]
  Siwen Yuan , Qilin Wu , TianpengYin . NMR Spectroscopy Teaching Design Using the Mosher Method for Stereochemistry of Organic Compounds Based on BOPPPS Teaching Model. University Chemistry, 2025, 40(7): 161-168. doi: 10.12461/PKU.DXHX202502073
19. [19]
  Mingyang Men , Jinghua Wu , Gaozhan Liu , Jing Zhang , Nini Zhang , Xiayin Yao . Sulfide Solid Electrolyte Synthesized by Liquid Phase Approach and Application in All-Solid-State Lithium Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100004-0. doi: 10.3866/PKU.WHXB202309019
20. [20]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(1347)
HTML views(147)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return